Characterization and Vaccine Potential of Outer Membrane Vesicles Produced by Haemophilus parasuis

Haemophilus parasuis is a Gram-negative bacterium that colonizes the upper respiratory tract of swine and is capable of causing a systemic infection, resulting in high morbidity and mortality. H. parasuis isolates display a wide range of virulence and virulence factors are largely unknown. Commercia...

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Published in:PloS one Vol. 11; no. 3; p. e0149132
Main Authors: McCaig, William D., Loving, Crystal L., Hughes, Holly R., Brockmeier, Susan L.
Format: Journal Article
Language:English
Published: United States Public Library of Science 01.03.2016
Public Library of Science (PLoS)
Subjects:
Animal diseases
Animals
Antibodies, Bacterial - blood
Apoptosis
Bacteria
Bacterial Outer Membrane Proteins - immunology
Bacterial Outer Membrane Proteins - isolation & purification
Bacterial vaccines
Bacterial Vaccines - immunology
Bacterial Vaccines - isolation & purification
Bacterins
BASIC BIOLOGICAL SCIENCES
Biology and Life Sciences
Cell adhesion & migration
Cross-reactivity
Cytokines - genetics
Cytokines - metabolism
Disseminated infection
Extracellular Vesicles - immunology
Genetic aspects
Haemophilus
Haemophilus Infections - blood
Haemophilus Infections - immunology
Haemophilus Infections - prevention & control
Haemophilus parasuis - immunology
Health aspects
Hemophilus infections
Hogs
Immune response
Immune system
Lethal dose
Livestock
Macrophages, Alveolar - immunology
Macrophages, Alveolar - metabolism
Medicine and Health Sciences
Membrane vesicles
Morbidity
outer membrane proteins
Pathogenesis
Physiological aspects
Prevention
proteases
Proteins
Proteolysis
Proteomics
Pseudomonas aeruginosa
Respiratory tract
serum proteins
Signaling
Sus scrofa
Swine
Toxins
Transcription, Genetic
Vaccination
vaccination and immunization
Vaccines
Vesicles
Vibrio cholerae
Virulence
Virulence (Microbiology)
Virulence factors
United States
ISSN:1932-6203, 1932-6203
Online Access:Get full text
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Abstract Haemophilus parasuis is a Gram-negative bacterium that colonizes the upper respiratory tract of swine and is capable of causing a systemic infection, resulting in high morbidity and mortality. H. parasuis isolates display a wide range of virulence and virulence factors are largely unknown. Commercial bacterins are often used to vaccinate swine against H. parasuis, though strain variability and lack of cross-reactivity can make this an ineffective means of protection. Outer membrane vesicles (OMV) are spherical structures naturally released from the membrane of bacteria and OMV are often enriched in toxins, signaling molecules and other bacterial components. Examination of OMV structures has led to identification of virulence factors in a number of bacteria and they have been successfully used as subunit vaccines. We have isolated OMV from both virulent and avirulent strains of H. parasuis, have examined their protein content and assessed their ability to induce an immune response in the host. Vaccination with purified OMV derived from the virulent H. parasuis Nagasaki strain provided protection against challenge with a lethal dose of the bacteria.
AbstractList Haemophilus parasuis is a Gram-negative bacterium that colonizes the upper respiratory tract of swine and is capable of causing a systemic infection, resulting in high morbidity and mortality. H. parasuis isolates display a wide range of virulence and virulence factors are largely unknown. Commercial bacterins are often used to vaccinate swine against H. parasuis, though strain variability and lack of cross-reactivity can make this an ineffective means of protection. Outer membrane vesicles (OMV) are spherical structures naturally released from the membrane of bacteria and OMV are often enriched in toxins, signaling molecules and other bacterial components. Examination of OMV structures has led to identification of virulence factors in a number of bacteria and they have been successfully used as subunit vaccines. We have isolated OMV from both virulent and avirulent strains of H. parasuis, have examined their protein content and assessed their ability to induce an immune response in the host. Vaccination with purified OMV derived from the virulent H. parasuis Nagasaki strain provided protection against challenge with a lethal dose of the bacteria.
Haemophilus parasuis is a Gram-negative bacterium that colonizes the upper respiratory tract of swine and is capable of causing a systemic infection, resulting in high morbidity and mortality. H. parasuis isolates display a wide range of virulence and virulence factors are largely unknown. Commercial bacterins are often used to vaccinate swine against H. parasuis, though strain variability and lack of cross-reactivity can make this an ineffective means of protection. Outer membrane vesicles (OMV) are spherical structures naturally released from the membrane of bacteria and OMV are often enriched in toxins, signaling molecules and other bacterial components. Examination of OMV structures has led to identification of virulence factors in a number of bacteria and they have been successfully used as subunit vaccines. We have isolated OMV from both virulent and avirulent strains of H. parasuis, have examined their protein content and assessed their ability to induce an immune response in the host. Lastly, vaccination with purified OMV derived from the virulent H. parasuis Nagasaki strain provided protection against challenge with a lethal dose of the bacteria.
Audience Academic
Author McCaig, William D.
Hughes, Holly R.
Loving, Crystal L.
Brockmeier, Susan L.
AuthorAffiliation Virus and Prion Diseases Research Unit, National Animal Disease Center, Agricultural Research Service, USDA, Ames, IA, United States of America
Université Paris Descartes, FRANCE
AuthorAffiliation_xml – name: Université Paris Descartes, FRANCE
– name: Virus and Prion Diseases Research Unit, National Animal Disease Center, Agricultural Research Service, USDA, Ames, IA, United States of America
Author_xml – sequence: 1
  givenname: William D.
  surname: McCaig
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– sequence: 2
  givenname: Crystal L.
  surname: Loving
  fullname: Loving, Crystal L.
– sequence: 3
  givenname: Holly R.
  surname: Hughes
  fullname: Hughes, Holly R.
– sequence: 4
  givenname: Susan L.
  surname: Brockmeier
  fullname: Brockmeier, Susan L.
BackLink https://www.ncbi.nlm.nih.gov/pubmed/26930282$$D View this record in MEDLINE/PubMed
https://www.osti.gov/servlets/purl/1252954$$D View this record in Osti.gov
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CorporateAuthor Oak Ridge Associated Universities (ORAU), Oak Ridge, TN (United States)
Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN (United States)
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USDOE
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Competing Interests: The authors have declared that no competing interests exist.
Conceived and designed the experiments: WM HH CL SB. Performed the experiments: WM SB. Analyzed the data: WM SB CL HH. Contributed reagents/materials/analysis tools: CL. Wrote the paper: WM CL SB.
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SSID ssj0053866
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Snippet Haemophilus parasuis is a Gram-negative bacterium that colonizes the upper respiratory tract of swine and is capable of causing a systemic infection, resulting...
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SubjectTerms Animal diseases
Animals
Antibodies, Bacterial - blood
Apoptosis
Bacteria
Bacterial Outer Membrane Proteins - immunology
Bacterial Outer Membrane Proteins - isolation & purification
Bacterial vaccines
Bacterial Vaccines - immunology
Bacterial Vaccines - isolation & purification
Bacterins
BASIC BIOLOGICAL SCIENCES
Biology and Life Sciences
Cell adhesion & migration
Cross-reactivity
Cytokines - genetics
Cytokines - metabolism
Disseminated infection
Extracellular Vesicles - immunology
Genetic aspects
Haemophilus
Haemophilus Infections - blood
Haemophilus Infections - immunology
Haemophilus Infections - prevention & control
Haemophilus parasuis - immunology
Health aspects
Hemophilus infections
Hogs
Immune response
Immune system
Lethal dose
Livestock
Macrophages, Alveolar - immunology
Macrophages, Alveolar - metabolism
Medicine and Health Sciences
Membrane vesicles
Morbidity
outer membrane proteins
Pathogenesis
Physiological aspects
Prevention
proteases
Proteins
Proteolysis
Proteomics
Pseudomonas aeruginosa
Respiratory tract
serum proteins
Signaling
Sus scrofa
Swine
Toxins
Transcription, Genetic
Vaccination
vaccination and immunization
Vaccines
Vesicles
Vibrio cholerae
Virulence
Virulence (Microbiology)
Virulence factors
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Title Characterization and Vaccine Potential of Outer Membrane Vesicles Produced by Haemophilus parasuis
URI https://www.ncbi.nlm.nih.gov/pubmed/26930282
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Volume 11
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