Characterization and Vaccine Potential of Outer Membrane Vesicles Produced by Haemophilus parasuis

Haemophilus parasuis is a Gram-negative bacterium that colonizes the upper respiratory tract of swine and is capable of causing a systemic infection, resulting in high morbidity and mortality. H. parasuis isolates display a wide range of virulence and virulence factors are largely unknown. Commercia...

Celý popis

Uloženo v:

Podrobná bibliografie
Vydáno v:	PloS one Ročník 11; číslo 3; s. e0149132
Hlavní autoři:	McCaig, William D., Loving, Crystal L., Hughes, Holly R., Brockmeier, Susan L.
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	United States Public Library of Science 01.03.2016 Public Library of Science (PLoS)
Témata:	Animal diseases Animals Antibodies, Bacterial - blood Apoptosis Bacteria Bacterial Outer Membrane Proteins - immunology Bacterial Outer Membrane Proteins - isolation & purification Bacterial vaccines Bacterial Vaccines - immunology Bacterial Vaccines - isolation & purification Bacterins BASIC BIOLOGICAL SCIENCES Biology and Life Sciences Cell adhesion & migration Cross-reactivity Cytokines - genetics Cytokines - metabolism Disseminated infection Extracellular Vesicles - immunology Genetic aspects Haemophilus Haemophilus Infections - blood Haemophilus Infections - immunology Haemophilus Infections - prevention & control Haemophilus parasuis - immunology Health aspects Hemophilus infections Hogs Immune response Immune system Lethal dose Livestock Macrophages, Alveolar - immunology Macrophages, Alveolar - metabolism Medicine and Health Sciences Membrane vesicles Morbidity outer membrane proteins Pathogenesis Physiological aspects Prevention proteases Proteins Proteolysis Proteomics Pseudomonas aeruginosa Respiratory tract serum proteins Signaling Sus scrofa Swine Toxins Transcription, Genetic Vaccination vaccination and immunization Vaccines Vesicles Vibrio cholerae Virulence Virulence (Microbiology) Virulence factors United States
ISSN:	1932-6203, 1932-6203
On-line přístup:	Získat plný text
Tagy:	Přidat tag Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!

Popis
Shrnutí:	Haemophilus parasuis is a Gram-negative bacterium that colonizes the upper respiratory tract of swine and is capable of causing a systemic infection, resulting in high morbidity and mortality. H. parasuis isolates display a wide range of virulence and virulence factors are largely unknown. Commercial bacterins are often used to vaccinate swine against H. parasuis, though strain variability and lack of cross-reactivity can make this an ineffective means of protection. Outer membrane vesicles (OMV) are spherical structures naturally released from the membrane of bacteria and OMV are often enriched in toxins, signaling molecules and other bacterial components. Examination of OMV structures has led to identification of virulence factors in a number of bacteria and they have been successfully used as subunit vaccines. We have isolated OMV from both virulent and avirulent strains of H. parasuis, have examined their protein content and assessed their ability to induce an immune response in the host. Vaccination with purified OMV derived from the virulent H. parasuis Nagasaki strain provided protection against challenge with a lethal dose of the bacteria.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 USDOE AC05-06OR23100 Competing Interests: The authors have declared that no competing interests exist. Conceived and designed the experiments: WM HH CL SB. Performed the experiments: WM SB. Analyzed the data: WM SB CL HH. Contributed reagents/materials/analysis tools: CL. Wrote the paper: WM CL SB.
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0149132