Polymersomes Prepared from Thermoresponsive Fluorescent Protein-Polymer Bioconjugates: Capture of and Report on Drug and Protein Payloads

Polymersomes provide a good platform for targeted drug delivery and the creation of complex (bio)catalytically active systems for research in synthetic biology. To realize these applications requires both spatial control over the encapsulation components in these polymersomes and a means to report w...

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Veröffentlicht in:Angewandte Chemie International Edition Jg. 54; H. 18; S. 5317 - 5322
Hauptverfasser: Wong, Chin Ken, Laos, Alistair J., Soeriyadi, Alexander H., Wiedenmann, Jörg, Curmi, Paul M. G., Gooding, J. Justin, Marquis, Christopher P., Stenzel, Martina H., Thordarson, Pall
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Weinheim WILEY-VCH Verlag 27.04.2015
WILEY‐VCH Verlag
Wiley Subscription Services, Inc
Ausgabe:International ed. in English
Schlagworte:
Acrylic Resins - chemical synthesis
Acrylic Resins - chemistry
compartmentalization
Doxorubicin
Doxorubicin - administration & dosage
Drug Carriers - chemical synthesis
Drug Carriers - chemistry
Drug Compounding
Drugs
Encapsulation
Energy transfer
Fluorescence
Fluorescence Resonance Energy Transfer
FRET
Green Fluorescent Proteins - chemistry
Hydrophobic and Hydrophilic Interactions
Luminescent Proteins - chemical synthesis
Luminescent Proteins - chemistry
Membranes
Microscopy
Microscopy, Confocal
Microscopy, Fluorescence
Particle Size
Payloads
Phase Transition
Phycoerythrin - chemistry
Polymers
polymersomes
protein modifications
Proteins
Self assembly
Surface Properties
Temperature
encapsulation
FRET
protein modifications
compartmentalization
polymersomes
ISSN:1433-7851, 1521-3773, 1521-3773
Online-Zugang:Volltext
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Zusammenfassung:Polymersomes provide a good platform for targeted drug delivery and the creation of complex (bio)catalytically active systems for research in synthetic biology. To realize these applications requires both spatial control over the encapsulation components in these polymersomes and a means to report where the components are in the polymersomes. To address these twin challenges, we synthesized the protein–polymer bioconjugate PNIPAM‐b‐amilFP497 composed of thermoresponsive poly(N‐isopropylacrylamide) (PNIPAM) and a green‐fluorescent protein variant (amilFP497). Above 37 °C, this bioconjugate forms polymersomes that can (co‐)encapsulate the fluorescent drug doxorubicin and the fluorescent light‐harvesting protein phycoerythrin 545 (PE545). Using fluorescence lifetime imaging microscopy and Förster resonance energy transfer (FLIM‐FRET), we can distinguish the co‐encapsulated PE545 protein inside the polymersome membrane while doxorubicin is found both in the polymersome core and membrane. Temperature‐induced self‐assembly: A temperature‐sensitive green fluorescent (amilFP497) protein–polymer bioconjugate forms polymersomes above 37 °C which encapsulate a mixture of pink fluorescent protein (PE545) and a red drug molecule (DOX). The spatial location of the payload is revealed by fluorescence lifetime microscopy.
Bibliographie:ArticleID:ANIE201412406
istex:D66F672E3FACD027C23FF5366DE37E6034FF9711
Australian Research Council (ARC) - No. CE140100036
ark:/67375/WNG-SZBH9ZXT-T
This work was supported by the Australian Research Council (ARC) centre of excellence grant (grant number CE140100036) to P.T. and J.J.G., an ARC Future Fellowship (FT120100101) to P.T., support from the Faculty of Science UNSW to C.P.M. and P.T. and the Australian Government for PhD scholarship to A.J.L.. C.K.W. is grateful for an ARC and UNSW PhD scholarship.
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ISSN:1433-7851
1521-3773
1521-3773
DOI:10.1002/anie.201412406