Polymersomes Prepared from Thermoresponsive Fluorescent Protein-Polymer Bioconjugates: Capture of and Report on Drug and Protein Payloads

Polymersomes provide a good platform for targeted drug delivery and the creation of complex (bio)catalytically active systems for research in synthetic biology. To realize these applications requires both spatial control over the encapsulation components in these polymersomes and a means to report w...

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Vydáno v:	Angewandte Chemie International Edition Ročník 54; číslo 18; s. 5317 - 5322
Hlavní autoři:	Wong, Chin Ken, Laos, Alistair J., Soeriyadi, Alexander H., Wiedenmann, Jörg, Curmi, Paul M. G., Gooding, J. Justin, Marquis, Christopher P., Stenzel, Martina H., Thordarson, Pall
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	Weinheim WILEY-VCH Verlag 27.04.2015 WILEY‐VCH Verlag Wiley Subscription Services, Inc
Vydání:	International ed. in English
Témata:	Acrylic Resins - chemical synthesis Acrylic Resins - chemistry compartmentalization Doxorubicin Doxorubicin - administration & dosage Drug Carriers - chemical synthesis Drug Carriers - chemistry Drug Compounding Drugs Encapsulation Energy transfer Fluorescence Fluorescence Resonance Energy Transfer FRET Green Fluorescent Proteins - chemistry Hydrophobic and Hydrophilic Interactions Luminescent Proteins - chemical synthesis Luminescent Proteins - chemistry Membranes Microscopy Microscopy, Confocal Microscopy, Fluorescence Particle Size Payloads Phase Transition Phycoerythrin - chemistry Polymers polymersomes protein modifications Proteins Self assembly Surface Properties Temperature encapsulation FRET protein modifications compartmentalization polymersomes
ISSN:	1433-7851, 1521-3773, 1521-3773
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Shrnutí:	Polymersomes provide a good platform for targeted drug delivery and the creation of complex (bio)catalytically active systems for research in synthetic biology. To realize these applications requires both spatial control over the encapsulation components in these polymersomes and a means to report where the components are in the polymersomes. To address these twin challenges, we synthesized the protein–polymer bioconjugate PNIPAM‐b‐amilFP497 composed of thermoresponsive poly(N‐isopropylacrylamide) (PNIPAM) and a green‐fluorescent protein variant (amilFP497). Above 37 °C, this bioconjugate forms polymersomes that can (co‐)encapsulate the fluorescent drug doxorubicin and the fluorescent light‐harvesting protein phycoerythrin 545 (PE545). Using fluorescence lifetime imaging microscopy and Förster resonance energy transfer (FLIM‐FRET), we can distinguish the co‐encapsulated PE545 protein inside the polymersome membrane while doxorubicin is found both in the polymersome core and membrane. Temperature‐induced self‐assembly: A temperature‐sensitive green fluorescent (amilFP497) protein–polymer bioconjugate forms polymersomes above 37 °C which encapsulate a mixture of pink fluorescent protein (PE545) and a red drug molecule (DOX). The spatial location of the payload is revealed by fluorescence lifetime microscopy.
Bibliografie:	ArticleID:ANIE201412406 istex:D66F672E3FACD027C23FF5366DE37E6034FF9711 Australian Research Council (ARC) - No. CE140100036 ark:/67375/WNG-SZBH9ZXT-T This work was supported by the Australian Research Council (ARC) centre of excellence grant (grant number CE140100036) to P.T. and J.J.G., an ARC Future Fellowship (FT120100101) to P.T., support from the Faculty of Science UNSW to C.P.M. and P.T. and the Australian Government for PhD scholarship to A.J.L.. C.K.W. is grateful for an ARC and UNSW PhD scholarship. ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23
ISSN:	1433-7851 1521-3773 1521-3773
DOI:	10.1002/anie.201412406