Clinically accurate diagnosis of Alzheimer’s disease via multiplexed sensing of core biomarkers in human plasma

Alzheimer’s disease (AD) is the most prevalent neurodegenerative disorder, affecting one in ten people aged over 65 years. Despite the severity of the disease, early diagnosis of AD is still challenging due to the low accuracy or high cost of neuropsychological tests and neuroimaging. Here we report...

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Veröffentlicht in:	Nature communications Jg. 11; H. 1; S. 119 - 9
Hauptverfasser:	Kim, Kayoung, Kim, Min-Ji, Kim, Da Won, Kim, Su Yeong, Park, Steve, Park, Chan Beum
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	London Nature Publishing Group UK 08.01.2020 Nature Publishing Group Nature Portfolio
Schlagworte:	147/135 147/143 147/3 631/61/350/59 639/301/357/73 692/699/375/365/1283 82/1 9/10 Accuracy Alignment Alzheimer Disease - blood Alzheimer Disease - diagnosis Alzheimer's disease Amyloid beta-Peptides - blood Biomarkers Biomarkers - blood Biosensing Techniques - instrumentation Biosensing Techniques - methods Blood Blood plasma Coefficient of variation Diagnosis Humanities and Social Sciences Humans Low concentrations Medical imaging Middle Aged multidisciplinary Nanotechnology Nanotubes Nanotubes, Carbon - chemistry Neurodegenerative diseases Neuroimaging Plasma Science Science (multidisciplinary) Selectivity Sensitivity Sensor arrays Sensors Single wall carbon nanotubes Tau protein tau Proteins - blood
ISSN:	2041-1723, 2041-1723
Online-Zugang:	Volltext
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Zusammenfassung:	Alzheimer’s disease (AD) is the most prevalent neurodegenerative disorder, affecting one in ten people aged over 65 years. Despite the severity of the disease, early diagnosis of AD is still challenging due to the low accuracy or high cost of neuropsychological tests and neuroimaging. Here we report clinically accurate and ultrasensitive detection of multiple AD core biomarkers (t-tau, p-tau 181 , Aβ 42 , and Aβ 40 ) in human plasma using densely aligned carbon nanotubes (CNTs). The closely packed and unidirectionally aligned CNT sensor array exhibits high precision, sensitivity, and accuracy, evidenced by a low coefficient of variation (<6%), a femtomolar-level limit of detection, and a high degree of recovery (>93.0%). By measuring the levels of t-tau/Aβ 42 , p-tau 181 /Aβ 42 , and Aβ 42 /Aβ 40 in clinical blood samples, the sensor array successfully discriminates the clinically diagnosed AD patients from healthy controls with an average sensitivity of 90.0%, a selectivity of 90.0%, and an average accuracy of 88.6%. Detection of Alzheimer’s disease (AD) biomarkers from patients’ blood is challenging because these are present in very low concentrations in the plasma. Here the authors develop a sensor array of densely aligned single-walled carbon nanotubes for clinically accurate detection of femtomolar AD biomarkers in human plasma samples.
Bibliographie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23
ISSN:	2041-1723 2041-1723
DOI:	10.1038/s41467-019-13901-z