Clinically accurate diagnosis of Alzheimer’s disease via multiplexed sensing of core biomarkers in human plasma

Alzheimer’s disease (AD) is the most prevalent neurodegenerative disorder, affecting one in ten people aged over 65 years. Despite the severity of the disease, early diagnosis of AD is still challenging due to the low accuracy or high cost of neuropsychological tests and neuroimaging. Here we report...

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Bibliographic Details
Published in:Nature communications Vol. 11; no. 1; pp. 119 - 9
Main Authors: Kim, Kayoung, Kim, Min-Ji, Kim, Da Won, Kim, Su Yeong, Park, Steve, Park, Chan Beum
Format: Journal Article
Language:English
Published: London Nature Publishing Group UK 08.01.2020
Nature Publishing Group
Nature Portfolio
Subjects:
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Accuracy
Alignment
Alzheimer Disease - blood
Alzheimer Disease - diagnosis
Alzheimer's disease
Amyloid beta-Peptides - blood
Biomarkers
Biomarkers - blood
Biosensing Techniques - instrumentation
Biosensing Techniques - methods
Blood
Blood plasma
Coefficient of variation
Diagnosis
Humanities and Social Sciences
Humans
Low concentrations
Medical imaging
Middle Aged
multidisciplinary
Nanotechnology
Nanotubes
Nanotubes, Carbon - chemistry
Neurodegenerative diseases
Neuroimaging
Plasma
Science
Science (multidisciplinary)
Selectivity
Sensitivity
Sensor arrays
Sensors
Single wall carbon nanotubes
Tau protein
tau Proteins - blood
ISSN:2041-1723, 2041-1723
Online Access:Get full text
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Description
Summary:Alzheimer’s disease (AD) is the most prevalent neurodegenerative disorder, affecting one in ten people aged over 65 years. Despite the severity of the disease, early diagnosis of AD is still challenging due to the low accuracy or high cost of neuropsychological tests and neuroimaging. Here we report clinically accurate and ultrasensitive detection of multiple AD core biomarkers (t-tau, p-tau 181 , Aβ 42 , and Aβ 40 ) in human plasma using densely aligned carbon nanotubes (CNTs). The closely packed and unidirectionally aligned CNT sensor array exhibits high precision, sensitivity, and accuracy, evidenced by a low coefficient of variation (<6%), a femtomolar-level limit of detection, and a high degree of recovery (>93.0%). By measuring the levels of t-tau/Aβ 42 , p-tau 181 /Aβ 42 , and Aβ 42 /Aβ 40 in clinical blood samples, the sensor array successfully discriminates the clinically diagnosed AD patients from healthy controls with an average sensitivity of 90.0%, a selectivity of 90.0%, and an average accuracy of 88.6%. Detection of Alzheimer’s disease (AD) biomarkers from patients’ blood is challenging because these are present in very low concentrations in the plasma. Here the authors develop a sensor array of densely aligned single-walled carbon nanotubes for clinically accurate detection of femtomolar AD biomarkers in human plasma samples.
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ISSN:2041-1723
2041-1723
DOI:10.1038/s41467-019-13901-z