Diversity of vaginal microbiome and metabolome during genital infections

We characterized the vaginal ecosystem during common infections of the female genital tract, as vulvovaginal candidiasis (VVC, n = 18) and Chlamydia trachomatis infection (CT, n = 20), recruiting healthy (HC, n = 21) and bacterial vaginosis-affected (BV, n = 20) women as references of eubiosis and d...

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Bibliographic Details
Published in:Scientific reports Vol. 9; no. 1; pp. 14095 - 12
Main Authors: Ceccarani, Camilla, Foschi, Claudio, Parolin, Carola, D’Antuono, Antonietta, Gaspari, Valeria, Consolandi, Clarissa, Laghi, Luca, Camboni, Tania, Vitali, Beatrice, Severgnini, Marco, Marangoni, Antonella
Format: Journal Article
Language:English
Published: London Nature Publishing Group UK 01.10.2019
Nature Publishing Group
Nature Portfolio
Subjects:
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631/326/2565/2134
692/699/255/1318
692/699/255/1672
Adult
Candidiasis
Candidiasis, Vulvovaginal - metabolism
Candidiasis, Vulvovaginal - microbiology
Case-Control Studies
Chlamydia Infections - metabolism
Chlamydia Infections - microbiology
Chlamydia trachomatis
Dysbacteriosis
Female
Genera
Genital tract
High-Throughput Nucleotide Sequencing
Humanities and Social Sciences
Humans
Infections
Lactic acid
Magnetic Resonance Spectroscopy
Metabolites
Metabolome
Metabolomics
Microbiomes
Microbiota - genetics
multidisciplinary
Next-generation sequencing
NMR
Nuclear magnetic resonance
Science
Science (multidisciplinary)
Sexually transmitted diseases
STD
Vagina
Vagina - metabolism
Vagina - microbiology
Vaginal Diseases - metabolism
Vaginal Diseases - microbiology
Vaginosis
Young Adult
ISSN:2045-2322, 2045-2322
Online Access:Get full text
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Summary:We characterized the vaginal ecosystem during common infections of the female genital tract, as vulvovaginal candidiasis (VVC, n = 18) and Chlamydia trachomatis infection (CT, n = 20), recruiting healthy (HC, n = 21) and bacterial vaginosis-affected (BV, n = 20) women as references of eubiosis and dysbiosis. The profiles of the vaginal microbiome and metabolome were studied in 79 reproductive-aged women, by means of next generation sequencing and proton based-nuclear magnetic resonance spectroscopy. Lactobacillus genus was profoundly depleted in all the genital infections herein considered, and species-level analysis revealed that healthy vaginal microbiome was dominated by L. crispatus . In the shift from HC to CT, VVC, and BV, L. crispatus was progressively replaced by L. iners . CT infection and VVC, as well as BV condition, were mainly characterised by anaerobe genera, e.g. Gardnerella , Prevotella , Megasphaera , Roseburia and Atopobium . The changes in the bacterial communities occurring during the genital infections resulted in significant alterations in the vaginal metabolites composition, being the decrease of lactate a common marker of all the pathological conditions. In conclusion, according to the taxonomic and metabolomics analysis, we found that each of the four conditions is characterized by a peculiar vaginal microbiome/metabolome fingerprint.
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ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-019-50410-x