Diversity of vaginal microbiome and metabolome during genital infections

We characterized the vaginal ecosystem during common infections of the female genital tract, as vulvovaginal candidiasis (VVC, n = 18) and Chlamydia trachomatis infection (CT, n = 20), recruiting healthy (HC, n = 21) and bacterial vaginosis-affected (BV, n = 20) women as references of eubiosis and d...

Celý popis

Uloženo v:

Podrobná bibliografie
Vydáno v:	Scientific reports Ročník 9; číslo 1; s. 14095 - 12
Hlavní autoři:	Ceccarani, Camilla, Foschi, Claudio, Parolin, Carola, D’Antuono, Antonietta, Gaspari, Valeria, Consolandi, Clarissa, Laghi, Luca, Camboni, Tania, Vitali, Beatrice, Severgnini, Marco, Marangoni, Antonella
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	London Nature Publishing Group UK 01.10.2019 Nature Publishing Group Nature Portfolio
Témata:	140/131 45 45/22 45/23 631/326/2565/2134 692/699/255/1318 692/699/255/1672 Adult Candidiasis Candidiasis, Vulvovaginal - metabolism Candidiasis, Vulvovaginal - microbiology Case-Control Studies Chlamydia Infections - metabolism Chlamydia Infections - microbiology Chlamydia trachomatis Dysbacteriosis Female Genera Genital tract High-Throughput Nucleotide Sequencing Humanities and Social Sciences Humans Infections Lactic acid Magnetic Resonance Spectroscopy Metabolites Metabolome Metabolomics Microbiomes Microbiota - genetics multidisciplinary Next-generation sequencing NMR Nuclear magnetic resonance Science Science (multidisciplinary) Sexually transmitted diseases STD Vagina Vagina - metabolism Vagina - microbiology Vaginal Diseases - metabolism Vaginal Diseases - microbiology Vaginosis Young Adult
ISSN:	2045-2322, 2045-2322
On-line přístup:	Získat plný text
Tagy:	Přidat tag Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!

Popis
Shrnutí:	We characterized the vaginal ecosystem during common infections of the female genital tract, as vulvovaginal candidiasis (VVC, n = 18) and Chlamydia trachomatis infection (CT, n = 20), recruiting healthy (HC, n = 21) and bacterial vaginosis-affected (BV, n = 20) women as references of eubiosis and dysbiosis. The profiles of the vaginal microbiome and metabolome were studied in 79 reproductive-aged women, by means of next generation sequencing and proton based-nuclear magnetic resonance spectroscopy. Lactobacillus genus was profoundly depleted in all the genital infections herein considered, and species-level analysis revealed that healthy vaginal microbiome was dominated by L. crispatus . In the shift from HC to CT, VVC, and BV, L. crispatus was progressively replaced by L. iners . CT infection and VVC, as well as BV condition, were mainly characterised by anaerobe genera, e.g. Gardnerella , Prevotella , Megasphaera , Roseburia and Atopobium . The changes in the bacterial communities occurring during the genital infections resulted in significant alterations in the vaginal metabolites composition, being the decrease of lactate a common marker of all the pathological conditions. In conclusion, according to the taxonomic and metabolomics analysis, we found that each of the four conditions is characterized by a peculiar vaginal microbiome/metabolome fingerprint.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23
ISSN:	2045-2322 2045-2322
DOI:	10.1038/s41598-019-50410-x