Metformin alleviates allergic airway inflammation and increases Treg cells in obese asthma

Obesity increases the morbidity and severity of asthma, with poor sensitivity to corticosteroid treatment. Metformin has potential effects on improving asthma airway inflammation. Regulatory T cells (Tregs) play a key role in suppressing the immunoreaction to allergens. We built an obese asthmatic m...

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Vydáno v:	Journal of cellular and molecular medicine Ročník 25; číslo 4; s. 2279 - 2284
Hlavní autoři:	Guo, Yimin, Shi, Jianting, Wang, Qiujie, Hong, Luna, Chen, Ming, Liu, Shanying, Yuan, Xiaoqing, Jiang, Shanping
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	England John Wiley & Sons, Inc 01.02.2021 John Wiley and Sons Inc
Témata:	Allergens Animals Anti-Inflammatory Agents - pharmacology Antidiabetics Asthma Asthma - drug therapy Asthma - immunology Asthma - pathology Binding sites Body weight Body Weight - drug effects Bronchoalveolar Lavage Fluid - chemistry Bronchoalveolar Lavage Fluid - immunology CD4 Lymphocyte Count Cholesterol Corticosteroids Cytokines Diet, High-Fat Disease Models, Animal Enzyme-linked immunosorbent assay Flow cytometry High fat diet Humans Hypoglycemic Agents - pharmacology Immunoregulation Inflammation Interleukin-4 - antagonists & inhibitors Interleukin-4 - immunology Interleukin-4 - metabolism Lipoproteins Lung - drug effects Lung - immunology Lung - pathology Lymphocytes T Metformin Metformin - pharmacology Mice Morbidity Obese asthma Obesity Obesity - drug therapy Obesity - immunology Obesity - pathology Ovalbumin Ovalbumin - administration & dosage Respiratory tract Respiratory tract diseases Short Communication Short Communications Software Spleen Spleen - drug effects Spleen - immunology Spleen - pathology T-Lymphocytes, Regulatory - drug effects T-Lymphocytes, Regulatory - immunology Transcription Transcription factors Tregs Tumor Necrosis Factor-alpha - antagonists & inhibitors Tumor Necrosis Factor-alpha - immunology Tumor Necrosis Factor-alpha - metabolism Tregs Obese asthma Metformin
ISSN:	1582-1838, 1582-4934, 1582-4934
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Popis
Shrnutí:	Obesity increases the morbidity and severity of asthma, with poor sensitivity to corticosteroid treatment. Metformin has potential effects on improving asthma airway inflammation. Regulatory T cells (Tregs) play a key role in suppressing the immunoreaction to allergens. We built an obese asthmatic mouse model by administering a high‐fat diet (HFD) and ovalbumin (OVA) sensitization, with daily metformin treatment. We measured the body weight and airway inflammatory status by histological analysis, qRT‐PCR, and ELISA. The percentage of Tregs was measured by flow cytometry. Obese asthmatic mice displayed more severe airway inflammation and more significant changes in inflammatory cytokines. Metformin reversed the obese situation and alleviated the airway inflammation and remodelling with increased Tregs and related transcript factors. The anti‐inflammatory function of metformin may be mediated by increasing Tregs.
Bibliografie:	Funding information This study was supported by National Nature Science Foundation of China (No.81670022, 81803636 and 8170010835), Nature Science Foundation of Guangdong Province (No.2017A030313822 and 2018A0303130329), Guangzhou Science and Technology Program Key Project (No.201704020123 and 202008040003) and Guangdong Key Technology R & D Program for Controlling 2019 n‐Cov (No.2020B111105001). ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 Yimin Guo and Jianting Shi contributed equally to this manuscript.
ISSN:	1582-1838 1582-4934 1582-4934
DOI:	10.1111/jcmm.16269