Metformin alleviates allergic airway inflammation and increases Treg cells in obese asthma

Obesity increases the morbidity and severity of asthma, with poor sensitivity to corticosteroid treatment. Metformin has potential effects on improving asthma airway inflammation. Regulatory T cells (Tregs) play a key role in suppressing the immunoreaction to allergens. We built an obese asthmatic m...

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Bibliographic Details
Published in:Journal of cellular and molecular medicine Vol. 25; no. 4; pp. 2279 - 2284
Main Authors: Guo, Yimin, Shi, Jianting, Wang, Qiujie, Hong, Luna, Chen, Ming, Liu, Shanying, Yuan, Xiaoqing, Jiang, Shanping
Format: Journal Article
Language:English
Published: England John Wiley & Sons, Inc 01.02.2021
John Wiley and Sons Inc
Subjects:
Allergens
Animals
Anti-Inflammatory Agents - pharmacology
Antidiabetics
Asthma
Asthma - drug therapy
Asthma - immunology
Asthma - pathology
Binding sites
Body weight
Body Weight - drug effects
Bronchoalveolar Lavage Fluid - chemistry
Bronchoalveolar Lavage Fluid - immunology
CD4 Lymphocyte Count
Cholesterol
Corticosteroids
Cytokines
Diet, High-Fat
Disease Models, Animal
Enzyme-linked immunosorbent assay
Flow cytometry
High fat diet
Humans
Hypoglycemic Agents - pharmacology
Immunoregulation
Inflammation
Interleukin-4 - antagonists & inhibitors
Interleukin-4 - immunology
Interleukin-4 - metabolism
Lipoproteins
Lung - drug effects
Lung - immunology
Lung - pathology
Lymphocytes T
Metformin
Metformin - pharmacology
Mice
Morbidity
Obese asthma
Obesity
Obesity - drug therapy
Obesity - immunology
Obesity - pathology
Ovalbumin
Ovalbumin - administration & dosage
Respiratory tract
Respiratory tract diseases
Short Communication
Short Communications
Software
Spleen
Spleen - drug effects
Spleen - immunology
Spleen - pathology
T-Lymphocytes, Regulatory - drug effects
T-Lymphocytes, Regulatory - immunology
Transcription
Transcription factors
Tregs
Tumor Necrosis Factor-alpha - antagonists & inhibitors
Tumor Necrosis Factor-alpha - immunology
Tumor Necrosis Factor-alpha - metabolism
Tregs
Obese asthma
Metformin
ISSN:1582-1838, 1582-4934, 1582-4934
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Description
Summary:Obesity increases the morbidity and severity of asthma, with poor sensitivity to corticosteroid treatment. Metformin has potential effects on improving asthma airway inflammation. Regulatory T cells (Tregs) play a key role in suppressing the immunoreaction to allergens. We built an obese asthmatic mouse model by administering a high‐fat diet (HFD) and ovalbumin (OVA) sensitization, with daily metformin treatment. We measured the body weight and airway inflammatory status by histological analysis, qRT‐PCR, and ELISA. The percentage of Tregs was measured by flow cytometry. Obese asthmatic mice displayed more severe airway inflammation and more significant changes in inflammatory cytokines. Metformin reversed the obese situation and alleviated the airway inflammation and remodelling with increased Tregs and related transcript factors. The anti‐inflammatory function of metformin may be mediated by increasing Tregs.
Bibliography:Funding information
This study was supported by National Nature Science Foundation of China (No.81670022, 81803636 and 8170010835), Nature Science Foundation of Guangdong Province (No.2017A030313822 and 2018A0303130329), Guangzhou Science and Technology Program Key Project (No.201704020123 and 202008040003) and Guangdong Key Technology R & D Program for Controlling 2019 n‐Cov (No.2020B111105001).
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Yimin Guo and Jianting Shi contributed equally to this manuscript.
ISSN:1582-1838
1582-4934
1582-4934
DOI:10.1111/jcmm.16269