Anti-inflammatory effects of chlorogenic acid from Taraxacum officinale on LTA-stimulated bovine mammary epithelial cells via the TLR2/NF-κB pathway

Mastitis is an inflammatory disease caused by microbial infection. Chlorogenic acid (CGA), one of the major phenolic acids in Taraxacum officinale , has natural antioxidant and anti-inflammatory properties in various cell types; however, the effects of CGA on Lipoteichoic acid (LTA)-induced bovine m...

Full description

Saved in:
Bibliographic Details
Published in:PloS one Vol. 18; no. 3; p. e0282343
Main Authors: Xu, Ping, Xu, Xiaobo, Fotina, Hanna, Fotina, Tetiana
Format: Journal Article
Language:English
Published: United States Public Library of Science 22.03.2023
Public Library of Science (PLoS)
Subjects:
Acids
Animals
Anti-inflammatory agents
Anti-Inflammatory Agents - pharmacology
Biology and Life Sciences
Cattle
Chlorogenic acid
Chlorogenic Acid - pharmacology
Chromatography
Dairy cattle
Dairy industry
Epithelial cells
Epithelial Cells - metabolism
Epithelium
Female
Gene expression
Gram-positive bacteria
High performance liquid chromatography
IL-1β
Infections
Inflammatory diseases
Interleukin 6
Interleukin-6 - genetics
Interleukin-6 - pharmacology
Kinases
Laboratories
Lipopolysaccharides - pharmacology
Lipoteichoic acid
Liquid chromatography
Mammary gland
Mammary Glands, Animal - metabolism
Mastitis
Medicine and Health Sciences
Microorganisms
Milk
NF-kappa B - metabolism
NF-κB protein
Pathways
Phenolic acids
Phenols
Phosphorylation
Proteins
Signal transduction
Signaling
Taraxacum
Taraxacum officinale
TLR2 protein
Toll-Like Receptor 2 - genetics
Toll-Like Receptor 2 - metabolism
Toll-like receptors
Tumor Necrosis Factor-alpha - metabolism
Tumor necrosis factor-α
United States > US
China
Japan
Ukraine
ISSN:1932-6203, 1932-6203
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Mastitis is an inflammatory disease caused by microbial infection. Chlorogenic acid (CGA), one of the major phenolic acids in Taraxacum officinale , has natural antioxidant and anti-inflammatory properties in various cell types; however, the effects of CGA on Lipoteichoic acid (LTA)-induced bovine mammary epithelial cells (BMECs) have not been investigated. In this study, the CGA content in T . officinale was determined by High-performance liquid chromatography (HPLC). BMECs were infected with LTA to induce the mastitis model. Different concentrations of CGA were administered after establishing the LTA infection. The results showed that the T . officinale contained CGA 1.36 mg/g. CGA significantly reduced the pro-inflammatory gene and protein expression of TNF-α, IL-6, and IL-1β. In addition, CGA downregulated the NO, TLR2, and NF-κB signaling pathways in LTA-infected bovine mammary epithelial cells. Our results indicate that CGA reduced the expression of TNF-α, IL-6, IL-1β, and TLR2 by inhibiting the phosphorylation of proteins in the NF-κB signaling pathways in a dose-dependent manner. This finding suggests that CGA may be a potential agent for the treatment of mastitis in dairy cows.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
Competing Interests: The authors have declared that no competing interests exist.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0282343