Anti-inflammatory effects of chlorogenic acid from Taraxacum officinale on LTA-stimulated bovine mammary epithelial cells via the TLR2/NF-κB pathway

Mastitis is an inflammatory disease caused by microbial infection. Chlorogenic acid (CGA), one of the major phenolic acids in Taraxacum officinale , has natural antioxidant and anti-inflammatory properties in various cell types; however, the effects of CGA on Lipoteichoic acid (LTA)-induced bovine m...

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Vydáno v:	PloS one Ročník 18; číslo 3; s. e0282343
Hlavní autoři:	Xu, Ping, Xu, Xiaobo, Fotina, Hanna, Fotina, Tetiana
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	United States Public Library of Science 22.03.2023 Public Library of Science (PLoS)
Témata:	Acids Animals Anti-inflammatory agents Anti-Inflammatory Agents - pharmacology Biology and Life Sciences Cattle Chlorogenic acid Chlorogenic Acid - pharmacology Chromatography Dairy cattle Dairy industry Epithelial cells Epithelial Cells - metabolism Epithelium Female Gene expression Gram-positive bacteria High performance liquid chromatography IL-1β Infections Inflammatory diseases Interleukin 6 Interleukin-6 - genetics Interleukin-6 - pharmacology Kinases Laboratories Lipopolysaccharides - pharmacology Lipoteichoic acid Liquid chromatography Mammary gland Mammary Glands, Animal - metabolism Mastitis Medicine and Health Sciences Microorganisms Milk NF-kappa B - metabolism NF-κB protein Pathways Phenolic acids Phenols Phosphorylation Proteins Signal transduction Signaling Taraxacum Taraxacum officinale TLR2 protein Toll-Like Receptor 2 - genetics Toll-Like Receptor 2 - metabolism Toll-like receptors Tumor Necrosis Factor-alpha - metabolism Tumor necrosis factor-α United States > US China Japan Ukraine
ISSN:	1932-6203, 1932-6203
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Shrnutí:	Mastitis is an inflammatory disease caused by microbial infection. Chlorogenic acid (CGA), one of the major phenolic acids in Taraxacum officinale , has natural antioxidant and anti-inflammatory properties in various cell types; however, the effects of CGA on Lipoteichoic acid (LTA)-induced bovine mammary epithelial cells (BMECs) have not been investigated. In this study, the CGA content in T . officinale was determined by High-performance liquid chromatography (HPLC). BMECs were infected with LTA to induce the mastitis model. Different concentrations of CGA were administered after establishing the LTA infection. The results showed that the T . officinale contained CGA 1.36 mg/g. CGA significantly reduced the pro-inflammatory gene and protein expression of TNF-α, IL-6, and IL-1β. In addition, CGA downregulated the NO, TLR2, and NF-κB signaling pathways in LTA-infected bovine mammary epithelial cells. Our results indicate that CGA reduced the expression of TNF-α, IL-6, IL-1β, and TLR2 by inhibiting the phosphorylation of proteins in the NF-κB signaling pathways in a dose-dependent manner. This finding suggests that CGA may be a potential agent for the treatment of mastitis in dairy cows.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 Competing Interests: The authors have declared that no competing interests exist.
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0282343