Portable Microfluidic Integrated Plasmonic Platform for Pathogen Detection

Timely detection of infectious agents is critical in early diagnosis and treatment of infectious diseases. Conventional pathogen detection methods, such as enzyme linked immunosorbent assay (ELISA), culturing or polymerase chain reaction (PCR) require long assay times and complex and expensive instr...

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Podrobná bibliografia
Vydané v:	Scientific reports Ročník 5; číslo 1; s. 9152
Hlavní autori:	Tokel, Onur, Yildiz, Umit Hakan, Inci, Fatih, Durmus, Naside Gozde, Ekiz, Okan Oner, Turker, Burak, Cetin, Can, Rao, Shruthi, Sridhar, Kaushik, Natarajan, Nalini, Shafiee, Hadi, Dana, Aykutlu, Demirci, Utkan
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	London Nature Publishing Group UK 24.03.2015 Nature Publishing Group
Predmet:	13/1 14 14/63 49/62 631/61 631/61/350 692/700 9/10 Antibodies, Bacterial - chemistry Buffers Colony Count, Microbial - instrumentation Dialysis Dialysis Solutions E coli Enzyme-linked immunosorbent assay Equipment Design Escherichia coli Escherichia coli - isolation & purification Humanities and Social Sciences Humans Infectious diseases Lab-On-A-Chip Devices Microfluidics multidisciplinary Pathogens Peritoneal dialysis Peritoneum Point-of-Care Systems Polymerase chain reaction Primary care Science Staphylococcus aureus Staphylococcus aureus - isolation & purification Surface plasmon resonance Surface Plasmon Resonance - instrumentation
ISSN:	2045-2322, 2045-2322
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Popis
Shrnutí:	Timely detection of infectious agents is critical in early diagnosis and treatment of infectious diseases. Conventional pathogen detection methods, such as enzyme linked immunosorbent assay (ELISA), culturing or polymerase chain reaction (PCR) require long assay times and complex and expensive instruments, which are not adaptable to point-of-care (POC) needs at resource-constrained as well as primary care settings. Therefore, there is an unmet need to develop simple, rapid and accurate methods for detection of pathogens at the POC. Here, we present a portable, multiplex, inexpensive microfluidic-integrated surface plasmon resonance (SPR) platform that detects and quantifies bacteria, i.e. , Escherichia coli ( E. coli ) and Staphylococcus aureus ( S. aureus ) rapidly. The platform presented reliable capture and detection of E. coli at concentrations ranging from ~10 5 to 3.2 × 10 7 CFUs/mL in phosphate buffered saline (PBS) and peritoneal dialysis (PD) fluid. The multiplexing and specificity capability of the platform was also tested with S. aureus samples. The presented platform technology could potentially be applicable to capture and detect other pathogens at the POC and primary care settings.
Bibliografia:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 These authors contributed equally to this work.
ISSN:	2045-2322 2045-2322
DOI:	10.1038/srep09152