Portable Microfluidic Integrated Plasmonic Platform for Pathogen Detection

Timely detection of infectious agents is critical in early diagnosis and treatment of infectious diseases. Conventional pathogen detection methods, such as enzyme linked immunosorbent assay (ELISA), culturing or polymerase chain reaction (PCR) require long assay times and complex and expensive instr...

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Veröffentlicht in:Scientific reports Jg. 5; H. 1; S. 9152
Hauptverfasser: Tokel, Onur, Yildiz, Umit Hakan, Inci, Fatih, Durmus, Naside Gozde, Ekiz, Okan Oner, Turker, Burak, Cetin, Can, Rao, Shruthi, Sridhar, Kaushik, Natarajan, Nalini, Shafiee, Hadi, Dana, Aykutlu, Demirci, Utkan
Format: Journal Article
Sprache:Englisch
Veröffentlicht: London Nature Publishing Group UK 24.03.2015
Nature Publishing Group
Schlagworte:
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Antibodies, Bacterial - chemistry
Buffers
Colony Count, Microbial - instrumentation
Dialysis
Dialysis Solutions
E coli
Enzyme-linked immunosorbent assay
Equipment Design
Escherichia coli
Escherichia coli - isolation & purification
Humanities and Social Sciences
Humans
Infectious diseases
Lab-On-A-Chip Devices
Microfluidics
multidisciplinary
Pathogens
Peritoneal dialysis
Peritoneum
Point-of-Care Systems
Polymerase chain reaction
Primary care
Science
Staphylococcus aureus
Staphylococcus aureus - isolation & purification
Surface plasmon resonance
Surface Plasmon Resonance - instrumentation
ISSN:2045-2322, 2045-2322
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Zusammenfassung:Timely detection of infectious agents is critical in early diagnosis and treatment of infectious diseases. Conventional pathogen detection methods, such as enzyme linked immunosorbent assay (ELISA), culturing or polymerase chain reaction (PCR) require long assay times and complex and expensive instruments, which are not adaptable to point-of-care (POC) needs at resource-constrained as well as primary care settings. Therefore, there is an unmet need to develop simple, rapid and accurate methods for detection of pathogens at the POC. Here, we present a portable, multiplex, inexpensive microfluidic-integrated surface plasmon resonance (SPR) platform that detects and quantifies bacteria, i.e. , Escherichia coli ( E. coli ) and Staphylococcus aureus ( S. aureus ) rapidly. The platform presented reliable capture and detection of E. coli at concentrations ranging from ~10 5 to 3.2 × 10 7  CFUs/mL in phosphate buffered saline (PBS) and peritoneal dialysis (PD) fluid. The multiplexing and specificity capability of the platform was also tested with S. aureus samples. The presented platform technology could potentially be applicable to capture and detect other pathogens at the POC and primary care settings.
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These authors contributed equally to this work.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep09152