Establishing a Dual Murine Model to Explore the Interactions Between Diabetes and Periodontitis in Mice

This study aimed to develop and validate a dual murine model integrating a high-fat diet (HFD) and a single streptozotocin (STZ) dose to induce diabetes mellitus (DM), alongside periodontitis (Perio) induced by ligature placement and oral inoculation with Porphyromonas gingivalis (P. gingivalis). Th...

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Published in:International journal of molecular sciences Vol. 26; no. 12; p. 5611
Main Authors: Silva, Bárbara R., Hidalgo, Marco A. R., Silva, Renata C. L., de Avila, Erica D., Fuentes, Deivys L. P., Carlos, Iracilda Z., Figueiredo, Ingrid D., Cerri, Estela S., Cerri, Paulo S., Baviera, Amanda M., de Molon, Rafael Scaf, Scarel-Caminaga, Raquel M.
Format: Journal Article
Language:English
Published: Switzerland MDPI AG 11.06.2025
MDPI
Subjects:
Alveolar Bone Loss - pathology
Analysis
Animals
Bacteroidaceae Infections - complications
Bacteroidaceae Infections - microbiology
Blood Glucose
Body fat
Carbohydrates
Collagen
Dextrose
Diabetes
Diabetes Mellitus, Experimental - complications
Diabetes Mellitus, Experimental - pathology
Diet, High-Fat - adverse effects
Disease Models, Animal
Enzymes
Global health
Glucose
Gum disease
Health aspects
Hyperglycemia
Inflammation
Insulin
Insulin Resistance
Liu, Timothy
Male
Metabolism
Mice
Mice, Inbred C57BL
Microbiota
Obesity
Pathogenesis
Periodontitis
Periodontitis - complications
Periodontitis - etiology
Periodontitis - metabolism
Periodontitis - microbiology
Periodontitis - pathology
Porphyromonas gingivalis
Public health
Streptozocin
Virulence
Weight control
X-Ray Microtomography
United States
Massachusetts
diabetes mellitus
mouse model
streptozotocin
ligature
periodontitis
ISSN:1422-0067, 1661-6596, 1422-0067
Online Access:Get full text
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Abstract This study aimed to develop and validate a dual murine model integrating a high-fat diet (HFD) and a single streptozotocin (STZ) dose to induce diabetes mellitus (DM), alongside periodontitis (Perio) induced by ligature placement and oral inoculation with Porphyromonas gingivalis (P. gingivalis). The goal was to mimic human pathological conditions, creating a physiologically relevant environment to study the interplay between DM and Perio. A total of 128 six-week-old male C57BL/6J mice were randomly divided into four groups: Control, DM, Perio, and DM-P. DM was induced by HFD and STZ injection, and Perio by ligature placement and P. gingivalis infection. Evaluations occurred at baseline and days 7, 14, and 21. Alveolar bone loss was assessed by micro-computed tomography, and inflammation was examined histologically. DM mice showed elevated glucose levels and insulin resistance. Perio and DM-P groups experienced significant bone loss compared with Control and DM groups. The morphometric analysis revealed abundant inflammatory cells and reduced collagen fibers in Perio and DM-P groups, especially at day 7. This dual murine model successfully replicated the key features of DM and Perio, maintaining overall health of the animals, and good tolerability by those subjects to the stress of both interventional procedures.
AbstractList This study aimed to develop and validate a dual murine model integrating a high-fat diet (HFD) and a single streptozotocin (STZ) dose to induce diabetes mellitus (DM), alongside periodontitis (Perio) induced by ligature placement and oral inoculation with Porphyromonas gingivalis (P. gingivalis). The goal was to mimic human pathological conditions, creating a physiologically relevant environment to study the interplay between DM and Perio. A total of 128 six-week-old male C57BL/6J mice were randomly divided into four groups: Control, DM, Perio, and DM-P. DM was induced by HFD and STZ injection, and Perio by ligature placement and P. gingivalis infection. Evaluations occurred at baseline and days 7, 14, and 21. Alveolar bone loss was assessed by micro-computed tomography, and inflammation was examined histologically. DM mice showed elevated glucose levels and insulin resistance. Perio and DM-P groups experienced significant bone loss compared with Control and DM groups. The morphometric analysis revealed abundant inflammatory cells and reduced collagen fibers in Perio and DM-P groups, especially at day 7. This dual murine model successfully replicated the key features of DM and Perio, maintaining overall health of the animals, and good tolerability by those subjects to the stress of both interventional procedures.
This study aimed to develop and validate a dual murine model integrating a high-fat diet (HFD) and a single streptozotocin (STZ) dose to induce diabetes mellitus (DM), alongside periodontitis (Perio) induced by ligature placement and oral inoculation with Porphyromonas gingivalis (P. gingivalis). The goal was to mimic human pathological conditions, creating a physiologically relevant environment to study the interplay between DM and Perio. A total of 128 six-week-old male C57BL/6J mice were randomly divided into four groups: Control, DM, Perio, and DM-P. DM was induced by HFD and STZ injection, and Perio by ligature placement and P. gingivalis infection. Evaluations occurred at baseline and days 7, 14, and 21. Alveolar bone loss was assessed by micro-computed tomography, and inflammation was examined histologically. DM mice showed elevated glucose levels and insulin resistance. Perio and DM-P groups experienced significant bone loss compared with Control and DM groups. The morphometric analysis revealed abundant inflammatory cells and reduced collagen fibers in Perio and DM-P groups, especially at day 7. This dual murine model successfully replicated the key features of DM and Perio, maintaining overall health of the animals, and good tolerability by those subjects to the stress of both interventional procedures.This study aimed to develop and validate a dual murine model integrating a high-fat diet (HFD) and a single streptozotocin (STZ) dose to induce diabetes mellitus (DM), alongside periodontitis (Perio) induced by ligature placement and oral inoculation with Porphyromonas gingivalis (P. gingivalis). The goal was to mimic human pathological conditions, creating a physiologically relevant environment to study the interplay between DM and Perio. A total of 128 six-week-old male C57BL/6J mice were randomly divided into four groups: Control, DM, Perio, and DM-P. DM was induced by HFD and STZ injection, and Perio by ligature placement and P. gingivalis infection. Evaluations occurred at baseline and days 7, 14, and 21. Alveolar bone loss was assessed by micro-computed tomography, and inflammation was examined histologically. DM mice showed elevated glucose levels and insulin resistance. Perio and DM-P groups experienced significant bone loss compared with Control and DM groups. The morphometric analysis revealed abundant inflammatory cells and reduced collagen fibers in Perio and DM-P groups, especially at day 7. This dual murine model successfully replicated the key features of DM and Perio, maintaining overall health of the animals, and good tolerability by those subjects to the stress of both interventional procedures.
This study aimed to develop and validate a dual murine model integrating a high-fat diet (HFD) and a single streptozotocin (STZ) dose to induce diabetes mellitus (DM), alongside periodontitis (Perio) induced by ligature placement and oral inoculation with ( ). The goal was to mimic human pathological conditions, creating a physiologically relevant environment to study the interplay between DM and Perio. A total of 128 six-week-old male C57BL/6J mice were randomly divided into four groups: Control, DM, Perio, and DM-P. DM was induced by HFD and STZ injection, and Perio by ligature placement and infection. Evaluations occurred at baseline and days 7, 14, and 21. Alveolar bone loss was assessed by micro-computed tomography, and inflammation was examined histologically. DM mice showed elevated glucose levels and insulin resistance. Perio and DM-P groups experienced significant bone loss compared with Control and DM groups. The morphometric analysis revealed abundant inflammatory cells and reduced collagen fibers in Perio and DM-P groups, especially at day 7. This dual murine model successfully replicated the key features of DM and Perio, maintaining overall health of the animals, and good tolerability by those subjects to the stress of both interventional procedures.
Audience Academic
Author Silva, Renata C. L.
de Molon, Rafael Scaf
Fuentes, Deivys L. P.
Cerri, Estela S.
Hidalgo, Marco A. R.
de Avila, Erica D.
Carlos, Iracilda Z.
Scarel-Caminaga, Raquel M.
Figueiredo, Ingrid D.
Baviera, Amanda M.
Silva, Bárbara R.
Cerri, Paulo S.
AuthorAffiliation 1 Department of Morphology, Genetics, Orthodontics and Pediatric Dentistry, School of Dentistry at Araraquara, São Paulo State University—UNESP, Araraquara 14801-903, SP, Brazil; barbara.roque@unesp.br (B.R.S.); marco.rimachi@unesp.br (M.A.R.H.); renata.cl.silva@unesp.br (R.C.L.S.); estela.sasso@unesp.br (E.S.C.); paulo.cerri@unesp.br (P.S.C.)
2 Department of Diagnosis and Surgery, School of Dentistry at Araçatuba, Sao Paulo State University—UNESP, Araçatuba 16015-050, SP, Brazil; erica.avila@unesp.br
3 Department of Clinical Analysis, School of Pharmaceutical Sciences, São Paulo State University—UNESP, Araraquara 14800-903, SP, Brazil; deivys.leandro@unesp.br (D.L.P.F.); iracilda.zeppone@unesp.br (I.Z.C.); ingrid.delbone@unesp.br (I.D.F.); amanda.baviera@unesp.br (A.M.B.)
AuthorAffiliation_xml – name: 1 Department of Morphology, Genetics, Orthodontics and Pediatric Dentistry, School of Dentistry at Araraquara, São Paulo State University—UNESP, Araraquara 14801-903, SP, Brazil; barbara.roque@unesp.br (B.R.S.); marco.rimachi@unesp.br (M.A.R.H.); renata.cl.silva@unesp.br (R.C.L.S.); estela.sasso@unesp.br (E.S.C.); paulo.cerri@unesp.br (P.S.C.)
– name: 3 Department of Clinical Analysis, School of Pharmaceutical Sciences, São Paulo State University—UNESP, Araraquara 14800-903, SP, Brazil; deivys.leandro@unesp.br (D.L.P.F.); iracilda.zeppone@unesp.br (I.Z.C.); ingrid.delbone@unesp.br (I.D.F.); amanda.baviera@unesp.br (A.M.B.)
– name: 2 Department of Diagnosis and Surgery, School of Dentistry at Araçatuba, Sao Paulo State University—UNESP, Araçatuba 16015-050, SP, Brazil; erica.avila@unesp.br
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Keywords diabetes mellitus
mouse model
streptozotocin
ligature
periodontitis
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RelatedPersons Liu, Timothy
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Snippet This study aimed to develop and validate a dual murine model integrating a high-fat diet (HFD) and a single streptozotocin (STZ) dose to induce diabetes...
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SubjectTerms Alveolar Bone Loss - pathology
Analysis
Animals
Bacteroidaceae Infections - complications
Bacteroidaceae Infections - microbiology
Blood Glucose
Body fat
Carbohydrates
Collagen
Dextrose
Diabetes
Diabetes Mellitus, Experimental - complications
Diabetes Mellitus, Experimental - pathology
Diet, High-Fat - adverse effects
Disease Models, Animal
Enzymes
Global health
Glucose
Gum disease
Health aspects
Hyperglycemia
Inflammation
Insulin
Insulin Resistance
Liu, Timothy
Male
Metabolism
Mice
Mice, Inbred C57BL
Microbiota
Obesity
Pathogenesis
Periodontitis
Periodontitis - complications
Periodontitis - etiology
Periodontitis - metabolism
Periodontitis - microbiology
Periodontitis - pathology
Porphyromonas gingivalis
Public health
Streptozocin
Virulence
Weight control
X-Ray Microtomography
Title Establishing a Dual Murine Model to Explore the Interactions Between Diabetes and Periodontitis in Mice
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