Establishing a Dual Murine Model to Explore the Interactions Between Diabetes and Periodontitis in Mice

This study aimed to develop and validate a dual murine model integrating a high-fat diet (HFD) and a single streptozotocin (STZ) dose to induce diabetes mellitus (DM), alongside periodontitis (Perio) induced by ligature placement and oral inoculation with Porphyromonas gingivalis (P. gingivalis). Th...

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Vydané v:International journal of molecular sciences Ročník 26; číslo 12; s. 5611
Hlavní autori: Silva, Bárbara R., Hidalgo, Marco A. R., Silva, Renata C. L., de Avila, Erica D., Fuentes, Deivys L. P., Carlos, Iracilda Z., Figueiredo, Ingrid D., Cerri, Estela S., Cerri, Paulo S., Baviera, Amanda M., de Molon, Rafael Scaf, Scarel-Caminaga, Raquel M.
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: Switzerland MDPI AG 11.06.2025
MDPI
Predmet:
Alveolar Bone Loss - pathology
Analysis
Animals
Bacteroidaceae Infections - complications
Bacteroidaceae Infections - microbiology
Blood Glucose
Body fat
Carbohydrates
Collagen
Dextrose
Diabetes
Diabetes Mellitus, Experimental - complications
Diabetes Mellitus, Experimental - pathology
Diet, High-Fat - adverse effects
Disease Models, Animal
Enzymes
Global health
Glucose
Gum disease
Health aspects
Hyperglycemia
Inflammation
Insulin
Insulin Resistance
Liu, Timothy
Male
Metabolism
Mice
Mice, Inbred C57BL
Microbiota
Obesity
Pathogenesis
Periodontitis
Periodontitis - complications
Periodontitis - etiology
Periodontitis - metabolism
Periodontitis - microbiology
Periodontitis - pathology
Porphyromonas gingivalis
Public health
Streptozocin
Virulence
Weight control
X-Ray Microtomography
United States
Massachusetts
diabetes mellitus
mouse model
streptozotocin
ligature
periodontitis
ISSN:1422-0067, 1661-6596, 1422-0067
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Shrnutí:This study aimed to develop and validate a dual murine model integrating a high-fat diet (HFD) and a single streptozotocin (STZ) dose to induce diabetes mellitus (DM), alongside periodontitis (Perio) induced by ligature placement and oral inoculation with Porphyromonas gingivalis (P. gingivalis). The goal was to mimic human pathological conditions, creating a physiologically relevant environment to study the interplay between DM and Perio. A total of 128 six-week-old male C57BL/6J mice were randomly divided into four groups: Control, DM, Perio, and DM-P. DM was induced by HFD and STZ injection, and Perio by ligature placement and P. gingivalis infection. Evaluations occurred at baseline and days 7, 14, and 21. Alveolar bone loss was assessed by micro-computed tomography, and inflammation was examined histologically. DM mice showed elevated glucose levels and insulin resistance. Perio and DM-P groups experienced significant bone loss compared with Control and DM groups. The morphometric analysis revealed abundant inflammatory cells and reduced collagen fibers in Perio and DM-P groups, especially at day 7. This dual murine model successfully replicated the key features of DM and Perio, maintaining overall health of the animals, and good tolerability by those subjects to the stress of both interventional procedures.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms26125611