An in vitro and in vivo Comparison of the Effect of Stevia rebaudiana Extracts on Different Caries-Related Variables: A Randomized Controlled Trial Pilot Study

The effect of Stevia extracts on in vitro Streptococcus mutans biofilm formation and in vivo plaque pH was evaluated in this paper. Three 10% solutions containing stevioside, rebaudioside A or sucrose were prepared. MTT assay was used to evaluate microbiological counts in vitro. Twenty volunteers ri...

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Published in:Caries research Vol. 48; no. 1; pp. 19 - 23
Main Authors: Brambilla, E., Cagetti, M.G., Ionescu, A., Campus, G., Lingström, P.
Format: Journal Article
Language:English
Published: Basel, Switzerland S. Karger AG 01.01.2014
Subjects:
2-YEAR
Adult
Bacterial Load - drug effects
Bacteriological Techniques
Biofilms - drug effects
Caries
Coloring Agents
Dental Caries - microbiology
Dental Plaque - physiopathology
DENTAL-CARIES
Diterpenes, Kaurane - pharmacology
Female
Follow-Up Studies
Glucosides - pharmacology
Humans
Hydrogen-Ion Concentration
INTENSE NATURAL SWEETENERS
Male
Mouthwashes - pharmacology
Odontologi
Odontology
Pilot Projects
Plant Extracts - pharmacology
PLAQUE-PH
Rebaudioside A
RISK
Short Communication
Spectrophotometry - methods
Stevia
Stevioside
Streptococcus mutans - drug effects
Streptococcus mutans - growth & development
SUCROSE
Sucrose - pharmacology
Sweetening Agents - pharmacology
SWEETNESS
Tetrazolium Salts
Thiazoles
Young Adult
Stevioside
Caries
Rebaudioside A
Plaque pH
ISSN:0008-6568, 1421-976X, 1421-976X
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Abstract The effect of Stevia extracts on in vitro Streptococcus mutans biofilm formation and in vivo plaque pH was evaluated in this paper. Three 10% solutions containing stevioside, rebaudioside A or sucrose were prepared. MTT assay was used to evaluate microbiological counts in vitro. Twenty volunteers rinsed for 1 min with each solutions, and plaque pH was measured at 7 time points after each rinse. Higher in vitro S. mutans biofilm formation was observed in sucrose solution (p < 0.01). After 5, 10, 15 and 30 min, the sucrose in vivo rinse produced a statistically significantly lower pH value compared to the Stevia extracts (F = 99.45, p < 0.01).Stevia extracts can be considered nonacidogenic.
AbstractList The effect of Stevia extracts on in vitro Streptococcus mutans biofilm formation and in vivo plaque pH was evaluated in this paper. Three 10% solutions containing stevioside, rebaudioside A or sucrose were prepared. MTT assay was used to evaluate microbiological counts in vitro. Twenty volunteers rinsed for 1 min with each solutions, and plaque pH was measured at 7 time points after each rinse. Higher in vitro S. mutans biofilm formation was observed in sucrose solution (p < 0.01). After 5, 10, 15 and 30 min, the sucrose in vivo rinse produced a statistically significantly lower pH value compared to the Stevia extracts (F = 99.45, p < 0.01).Stevia extracts can be considered nonacidogenic.
The effect of Stevia extracts on in vitro Streptococcus mutans biofilm formation and in vivo plaque pH was evaluated in this paper. Three 10% solutions containing stevioside, rebaudioside A or sucrose were prepared. MTT assay was used to evaluate microbiological counts in vitro. Twenty volunteers rinsed for 1 min with each solutions, and plaque pH was measured at 7 time points after each rinse. Higher in vitro S. mutans biofilm formation was observed in sucrose solution (p < 0.01). After 5, 10, 15 and 30 min, the sucrose in vivo rinse produced a statistically significantly lower pH value compared to the Stevia extracts (F = 99.45, p < 0.01).Stevia extracts can be considered nonacidogenic.The effect of Stevia extracts on in vitro Streptococcus mutans biofilm formation and in vivo plaque pH was evaluated in this paper. Three 10% solutions containing stevioside, rebaudioside A or sucrose were prepared. MTT assay was used to evaluate microbiological counts in vitro. Twenty volunteers rinsed for 1 min with each solutions, and plaque pH was measured at 7 time points after each rinse. Higher in vitro S. mutans biofilm formation was observed in sucrose solution (p < 0.01). After 5, 10, 15 and 30 min, the sucrose in vivo rinse produced a statistically significantly lower pH value compared to the Stevia extracts (F = 99.45, p < 0.01).Stevia extracts can be considered nonacidogenic.
The effect of Stevia extracts on in vitro Streptococcus mutans biofilm formation and in vivo plaque pH was evaluated in this paper. Three 10% solutions containing stevioside, rebaudioside A or sucrose were prepared. MTT assay was used to evaluate microbiological counts in vitro. Twenty volunteers rinsed for 1 min with each solutions, and plaque pH was measured at 7 time points after each rinse. Higher in vitro S. mutans biofilm formation was observed in sucrose solution (p < 0.01). After 5, 10, 15 and 30 min, the sucrose in vivo rinse produced a statistically significantly lower pH value compared to the Stevia extracts (F = 99.45, p < 0.01).Stevia extracts can be considered nonacidogenic. © 2013 S. Karger AG, Basel [PUBLICATION ABSTRACT]
The effect of Stevia extracts on in vitro Streptococcus mutans biofilm formation and in vivo plaque pH was evaluated in this paper. Three 10% solutions containing stevioside, rebaudioside A or sucrose were prepared. MIT assay was used to evaluate microbiological counts in vitro. Twenty volunteers rinsed for 1 min with each solutions, and plaque pH was measured at 7 time points after each rinse. Higher in vitro S. mutans biofilm formation was observed in sucrose solution (p < 0.01). After 5, 10, 15 and 30 min, the sucrose in vivo rinse produced a statistically significantly lower pH value compared to the Stevia extracts (F = 99.45, p < 0.01). Stevia extracts can be considered nonacidogenic. (C) 20135. Karger AG, Basel
Author Lingström, P.
Campus, G.
Cagetti, M.G.
Ionescu, A.
Brambilla, E.
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  surname: Ionescu
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  surname: Campus
  fullname: Campus, G.
– sequence: 5
  givenname: P.
  surname: Lingström
  fullname: Lingström, P.
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Issue 1
Keywords Stevioside
Caries
Rebaudioside A
Plaque pH
Language English
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PublicationTitle Caries research
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Snippet The effect of Stevia extracts on in vitro Streptococcus mutans biofilm formation and in vivo plaque pH was evaluated in this paper. Three 10% solutions...
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StartPage 19
SubjectTerms 2-YEAR
Adult
Bacterial Load - drug effects
Bacteriological Techniques
Biofilms - drug effects
Caries
Coloring Agents
Dental Caries - microbiology
Dental Plaque - physiopathology
DENTAL-CARIES
Diterpenes, Kaurane - pharmacology
Female
Follow-Up Studies
Glucosides - pharmacology
Humans
Hydrogen-Ion Concentration
INTENSE NATURAL SWEETENERS
Male
Mouthwashes - pharmacology
Odontologi
Odontology
Pilot Projects
Plant Extracts - pharmacology
PLAQUE-PH
Rebaudioside A
RISK
Short Communication
Spectrophotometry - methods
Stevia
Stevioside
Streptococcus mutans - drug effects
Streptococcus mutans - growth & development
SUCROSE
Sucrose - pharmacology
Sweetening Agents - pharmacology
SWEETNESS
Tetrazolium Salts
Thiazoles
Young Adult
Title An in vitro and in vivo Comparison of the Effect of Stevia rebaudiana Extracts on Different Caries-Related Variables: A Randomized Controlled Trial Pilot Study
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Volume 48
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