Transfection of cultured fish cells RBCF-1 with exogenous oncogene and their resistance to malignant transformation

1. Plasmids bearing the G418-resistant gene neo were transfected into cultured fish cells RBCF-1 by electroporation at an efficiency comparable to that in NIH3T3 cells. 2. Transfection of plasmids bearing both neo and activated human c-Ha-ras into NIH3T3 and RBCF-1 cells resulted in the malignant tr...

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Bibliographic Details
Published in:Comparative biochemistry and physiology. B, Comparative biochemistry Vol. 96; no. 2; p. 349
Main Authors: Hayasaka, K, Sato, M, Mitani, H, Shima, A
Format: Journal Article
Language:English
Published: England 1990
Subjects:
Animals
Anti-Bacterial Agents
Cell Division
Cell Line
Cell Transformation, Neoplastic
Cells, Cultured
Drug Resistance - genetics
Genes, ras
Gentamicins
Goldfish
Humans
Mice
Transfection
Urinary Bladder Neoplasms
ISSN:0305-0491
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Summary:1. Plasmids bearing the G418-resistant gene neo were transfected into cultured fish cells RBCF-1 by electroporation at an efficiency comparable to that in NIH3T3 cells. 2. Transfection of plasmids bearing both neo and activated human c-Ha-ras into NIH3T3 and RBCF-1 cells resulted in the malignant transformation of the former but not of the latter cells.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0305-0491
DOI:10.1016/0305-0491(90)90387-9