Transfection of cultured fish cells RBCF-1 with exogenous oncogene and their resistance to malignant transformation

1. Plasmids bearing the G418-resistant gene neo were transfected into cultured fish cells RBCF-1 by electroporation at an efficiency comparable to that in NIH3T3 cells. 2. Transfection of plasmids bearing both neo and activated human c-Ha-ras into NIH3T3 and RBCF-1 cells resulted in the malignant tr...

Celý popis

Uloženo v:
Podrobná bibliografie
Vydáno v:Comparative biochemistry and physiology. B, Comparative biochemistry Ročník 96; číslo 2; s. 349
Hlavní autoři: Hayasaka, K, Sato, M, Mitani, H, Shima, A
Médium: Journal Article
Jazyk:angličtina
Vydáno: England 1990
Témata:
Animals
Anti-Bacterial Agents
Cell Division
Cell Line
Cell Transformation, Neoplastic
Cells, Cultured
Drug Resistance - genetics
Genes, ras
Gentamicins
Goldfish
Humans
Mice
Transfection
Urinary Bladder Neoplasms
ISSN:0305-0491
On-line přístup:Zjistit podrobnosti o přístupu
Tagy: Přidat tag
Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!
Popis
Shrnutí:1. Plasmids bearing the G418-resistant gene neo were transfected into cultured fish cells RBCF-1 by electroporation at an efficiency comparable to that in NIH3T3 cells. 2. Transfection of plasmids bearing both neo and activated human c-Ha-ras into NIH3T3 and RBCF-1 cells resulted in the malignant transformation of the former but not of the latter cells.
Bibliografie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0305-0491
DOI:10.1016/0305-0491(90)90387-9