Transfection of cultured fish cells RBCF-1 with exogenous oncogene and their resistance to malignant transformation

1. Plasmids bearing the G418-resistant gene neo were transfected into cultured fish cells RBCF-1 by electroporation at an efficiency comparable to that in NIH3T3 cells. 2. Transfection of plasmids bearing both neo and activated human c-Ha-ras into NIH3T3 and RBCF-1 cells resulted in the malignant tr...

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Bibliographic Details

Published in:	Comparative biochemistry and physiology. B, Comparative biochemistry Vol. 96; no. 2; p. 349
Main Authors:	Hayasaka, K, Sato, M, Mitani, H, Shima, A
Format:	Journal Article
Language:	English
Published:	England 1990
Subjects:	Animals Anti-Bacterial Agents Cell Division Cell Line Cell Transformation, Neoplastic Cells, Cultured Drug Resistance - genetics Genes, ras Gentamicins Goldfish Humans Mice Transfection Urinary Bladder Neoplasms
ISSN:	0305-0491
Online Access:	Get more information
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