Transfection of cultured fish cells RBCF-1 with exogenous oncogene and their resistance to malignant transformation

1. Plasmids bearing the G418-resistant gene neo were transfected into cultured fish cells RBCF-1 by electroporation at an efficiency comparable to that in NIH3T3 cells. 2. Transfection of plasmids bearing both neo and activated human c-Ha-ras into NIH3T3 and RBCF-1 cells resulted in the malignant tr...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Comparative biochemistry and physiology. B, Comparative biochemistry Jg. 96; H. 2; S. 349
Hauptverfasser: Hayasaka, K, Sato, M, Mitani, H, Shima, A
Format: Journal Article
Sprache:Englisch
Veröffentlicht: England 1990
Schlagworte:
Animals
Anti-Bacterial Agents
Cell Division
Cell Line
Cell Transformation, Neoplastic
Cells, Cultured
Drug Resistance - genetics
Genes, ras
Gentamicins
Goldfish
Humans
Mice
Transfection
Urinary Bladder Neoplasms
ISSN:0305-0491
Online-Zugang:Weitere Angaben
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:1. Plasmids bearing the G418-resistant gene neo were transfected into cultured fish cells RBCF-1 by electroporation at an efficiency comparable to that in NIH3T3 cells. 2. Transfection of plasmids bearing both neo and activated human c-Ha-ras into NIH3T3 and RBCF-1 cells resulted in the malignant transformation of the former but not of the latter cells.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0305-0491
DOI:10.1016/0305-0491(90)90387-9