Exploratory study on detection of drug resistance of Mycobacterium tuberculosis in sputum specimens by next-generation sequencing

To compare the diagnostic performance of next-generation sequencing (NGS) detection methods in sputum samples and strains, in order to explore the feasibility of the NGS method to detect drug resistance in sputum specimens. In this retrospective study, the sputum specimens and corresponding clinical...

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Bibliographic Details
Published in:Zhonghua jiehe he huxi zazhi Vol. 45; no. 6; p. 552
Main Authors: Liu, Y Y, Shi, J, Chu, P, Wu, T Y, Li, L, Pang, Y, Lu, J, Guo, Y L
Format: Journal Article
Language:Chinese
Published: China 12.06.2022
Subjects:
Amikacin - pharmacology
Antitubercular Agents - pharmacology
Antitubercular Agents - therapeutic use
Capreomycin - pharmacology
Drug Resistance, Multiple, Bacterial - genetics
High-Throughput Nucleotide Sequencing - methods
Humans
Isoniazid - pharmacology
Kanamycin - pharmacology
Levofloxacin - pharmacology
Microbial Sensitivity Tests
Mycobacterium tuberculosis
Retrospective Studies
Rifampin - pharmacology
Sputum - microbiology
Streptomycin - pharmacology
Tuberculosis, Lymph Node
Tuberculosis, Multidrug-Resistant - diagnosis
ISSN:1001-0939
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Summary:To compare the diagnostic performance of next-generation sequencing (NGS) detection methods in sputum samples and strains, in order to explore the feasibility of the NGS method to detect drug resistance in sputum specimens. In this retrospective study, the sputum specimens and corresponding clinical isolates of 50 pulmonary tuberculosis patients admitted to Beijing Chest Hospital from January 2017 to December 2017 were collected. The gene mutations of , , , , , , , , and in sputum specimens and corresponding clinical isolates were detected by NGS method. The phenotypic drug susceptibility test (DST) of the strains was carried out by the proportion method. Using DST results as a reference, the sensitivity, specificity, positive predictive value and negative predictive value of the NGS method for clinical strains and sputum specimens, as well as the consistency statistic ( ) with phenotype DST were calculated respectively. The Chi-square test was used to compare the accuracy of the NGS testing in sputum samples
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ISSN:1001-0939
DOI:10.3760/cma.j.cn112147-20211104-00775