Apoptosis in ischemia/reperfusion injury of human renal allografts

Ischemia/reperfusion injury of human renal allografts has a number of clinically significant consequences. A number of mechanisms of ischemia/ reperfusion injury have been elucidated, and there is evidence that apoptosis may be a contributing factor. To examine immediate posttransplant events, fixed...

Celý popis

Uloženo v:
Podrobná bibliografie
Vydáno v:Transplantation Ročník 66; číslo 7; s. 872
Hlavní autoři: Burns, A T, Davies, D R, McLaren, A J, Cerundolo, L, Morris, P J, Fuggle, S V
Médium: Journal Article
Jazyk:angličtina
Vydáno: United States 15.10.1998
Témata:
Adolescent
Adult
Apoptosis - physiology
Biopsy
Female
Humans
Ischemia - pathology
Ischemia - physiopathology
Kidney - pathology
Kidney - physiopathology
Kidney Transplantation
Male
Middle Aged
Postoperative Period
Renal Circulation - physiology
Reperfusion Injury - pathology
Reperfusion Injury - physiopathology
Transplantation, Homologous
ISSN:0041-1337
On-line přístup:Zjistit podrobnosti o přístupu
Tagy: Přidat tag
Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!
Popis
Shrnutí:Ischemia/reperfusion injury of human renal allografts has a number of clinically significant consequences. A number of mechanisms of ischemia/ reperfusion injury have been elucidated, and there is evidence that apoptosis may be a contributing factor. To examine immediate posttransplant events, fixed tissue sections from paraffin-embedded wedge biopsy specimens taken before and after reperfusion of human renal allografts were stained using terminal deoxytransferase-mediated dUTP nick-end labeling to detect the DNA fragmentation characteristic of apoptosis. Thirty-six pairs of pre- and postreperfusion biopsy specimens were examined, 11 from living-related donor renal transplants and 25 from cadaveric donor transplants. Quantitation of the terminal deoxytransferase-mediated dUTP nick-end labeling signal showed that significantly more apoptosis occurred in postreperfusion compared with prereperfusion biopsy specimens from cadaveric donor transplants, but a similar difference was not observed in living-related donor renal transplants. Furthermore, significantly more apoptosis was observed in postreperfusion biopsy specimens from cadaveric compared with living-related renal transplants. Postreperfusion biopsy specimens from kidneys that were cold preserved longer than 30 hr had a higher mean apoptosis score than those stored for less than 24 hr, but the result was not statistically significant. Thus, apoptosis occurs predominantly as a result of reperfusion after cold preservation of cadaveric donor renal allografts and provides additional information regarding the extent of ischemia/ reperfusion injury in an organ. The clinical value of this information remains to be determined.
Bibliografie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0041-1337
DOI:10.1097/00007890-199810150-00010