Principles and clinical applications of liquid chromatography — tandem mass spectrometry for the determination of adrenal and gonadal steroid hormones

Liquid-chromatography — tandem mass spectrometry (LC-MS/MS) is becoming the method of choice for clinical steroid analysis. In most instances, it has the advantage of higher sensitivity, better reproducibility and greater specificity than commercial immunoassay techniques. The method requires only m...

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Bibliographic Details
Published in:Journal of endocrinological investigation Vol. 34; no. 9; pp. 702 - 708
Main Authors: Kulle, A. E., Welzel, M., Holterhus, P.-M., Riepe, F. G.
Format: Journal Article
Language:English
Published: Cham Springer International Publishing 01.10.2011
Subjects:
Adrenal Gland Diseases - diagnosis
Adrenal Glands - chemistry
Chromatography, Liquid - economics
Chromatography, Liquid - instrumentation
Chromatography, Liquid - methods
Endocrinology
Endocrinology - economics
Endocrinology - methods
Gonadal Steroid Hormones - analysis
Humans
Immunoassay - economics
Immunoassay - instrumentation
Immunoassay - methods
Medicine
Medicine & Public Health
Metabolic Diseases
Molecular Structure
Reference Values
Sensitivity and Specificity
Short Review
Steroids - analysis
Tandem Mass Spectrometry - economics
Tandem Mass Spectrometry - instrumentation
Tandem Mass Spectrometry - methods
clinical
gonadal
tandem mass spectrometry
application
Adrenal
steroid
ISSN:0391-4097, 1720-8386, 1720-8386
Online Access:Get full text
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Summary:Liquid-chromatography — tandem mass spectrometry (LC-MS/MS) is becoming the method of choice for clinical steroid analysis. In most instances, it has the advantage of higher sensitivity, better reproducibility and greater specificity than commercial immunoassay techniques. The method requires only minimal sample preparation and a small sample volume. Furthermore, it has the potential to analyze multiple steroids simultaneously. Modern instruments guarantee high throughput, allowing an affordable price for the individual assay. All this makes LC-MS/MS an attractive method for use in a clinical setting. Reliable reference ranges for the detected analytes are the pre-requisite for their clinical use. If these are available, LC-MS/MS can find application in congenital disorders of steroid metabolism, such as congenital adrenal hyperplasia, disorders of sex development and disorders of salt homeostasis, as well as in acquired disorders of steroid metabolism, such as primary aldosteronism, Cushing’s disease, Addison’s disease, and hyperandrogenemia, as well as in psychiatric disease states such as depression or anxiety disorders. The principles of LC-MS/MS for steroid measurement, the pros and cons of LC-MS/MS compared with conventional immunoassays and the possible applications in clinical routine, with a special focus on pediatric endocrinology needs, are discussed here.
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ISSN:0391-4097
1720-8386
1720-8386
DOI:10.3275/7843