Human immunodeficiency virus type-1 reverse transcriptase. Contribution of Met-184 to binding of nucleoside 5'-triphosphate

Mutations were made in recombinant human immunodeficiency virus type-1 reverse transcriptase (RT) by substituting methionine 184 with alanine (M184A) or valine (M184V), and steady-state and pre-steady-state kinetic constants were determined. The Km values of M184A RT for dNTPs were larger than those...

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Bibliographic Details
Published in:	The Journal of biological chemistry Vol. 271; no. 23; p. 13656
Main Authors:	Wilson, J E, Aulabaugh, A, Caligan, B, McPherson, S, Wakefield, J K, Jablonski, S, Morrow, C D, Reardon, J E, Furman, P A
Format:	Journal Article
Language:	English
Published:	United States 07.06.1996
Subjects:	Amino Acid Sequence Base Sequence Binding Sites - genetics Conserved Sequence DNA Primers - genetics HIV Reverse Transcriptase HIV-1 - enzymology HIV-1 - genetics Humans In Vitro Techniques Kinetics Molecular Sequence Data Nucleotides - metabolism Point Mutation Recombinant Proteins - genetics Recombinant Proteins - metabolism RNA-Directed DNA Polymerase - genetics RNA-Directed DNA Polymerase - metabolism Substrate Specificity
ISSN:	0021-9258
Online Access:	Get more information
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