Generation of Induced Pluripotent Stem Cell-Like Lines from Human Pancreatic Ductal Adenocarcinoma

Pancreatic ductal adenocarcinoma (PDAC) has a dismal prognosis, mainly because the tumors are detected too late for effective treatment or for developing suitable therapeutics. Reprogramming cancer cells to pluripotency by induced pluripotent stem cell (iPSC) technology, which can be then programmed...

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Vydané v:Methods in molecular biology (Clifton, N.J.) Ročník 1882; s. 33
Hlavní autori: Kim, Jungsun, Zaret, Kenneth S
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: United States 2019
Predmet:
Animals
Carcinoma, Pancreatic Ductal - pathology
Carcinoma, Pancreatic Ductal - therapy
Cell Culture Techniques - instrumentation
Cell Culture Techniques - methods
Cell Differentiation - genetics
Cell Line, Tumor
Cellular Reprogramming Techniques - methods
Fibroblasts
Genetic Vectors - genetics
HEK293 Cells
Humans
Induced Pluripotent Stem Cells - pathology
Lentivirus - genetics
Mice
Pancreatic Neoplasms - pathology
Pancreatic Neoplasms - therapy
Transcription Factors - genetics
Transduction, Genetic - instrumentation
Transduction, Genetic - methods
Xenograft Model Antitumor Assays - methods
Induced pluripotent stem cell
Pancreatic ductal adenocarcinoma
Pancreatic intraepithelial neoplasia
ISSN:1940-6029, 1940-6029
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Shrnutí:Pancreatic ductal adenocarcinoma (PDAC) has a dismal prognosis, mainly because the tumors are detected too late for effective treatment or for developing suitable therapeutics. Reprogramming cancer cells to pluripotency by induced pluripotent stem cell (iPSC) technology, which can be then programmed back to their original cellular state, allows for studying the dynamic events in the course of the disease progression. Thus, we applied iPSC technology to model early progression of PDAC. We showed that when an iPS-like cell line, designated 10-22, derived from human recurrent PDAC, was injected into immunodeficient mice, the cells consistently recapitulated preinvasive, pancreatic intraepithelial neoplasia (PanIN) to invasive stages of human PDAC. This model was recently validated by revealing a new biomarker that can classify early resectable PDAC patients from healthy subjects. The procedure to derive iPSCs from human PDAC is principally the same as the procedure to generate iPSCs from normal human fibroblast. However, the heterogeneous initial populations, different cellular states, and active memory of pancreatic epithelial cells challenge for making iPSC-like lines from human PDAC. Herein, we describe how to create and maintain iPSC-like line from human PDAC by lentiviral transduction of reprogramming factors.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:1940-6029
1940-6029
DOI:10.1007/978-1-4939-8879-2_4