Evolutionary relationship and secondary structure predictions in four transport proteins of Saccharomyces cerevisiae

The comparison of the amino acid sequences of four yeast transport proteins indicates that there is a questionable relatedness between the uracil permease (FUR4) and the purine-cytosine permease (FCY2), whereas the arginine permease (CAN1) and the histidine permease (HIP1) clearly originated from a...

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Vydané v:Journal of molecular evolution Ročník 27; číslo 4; s. 341
Hlavní autori: Weber, E, Chevallier, M R, Jund, R
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: Germany 1988
Predmet:
Amino Acid Sequence
Base Sequence
Biological Evolution
Cell Membrane - enzymology
Genes
Genes, Fungal
Membrane Transport Proteins - genetics
Molecular Sequence Data
Protein Conformation
Saccharomyces cerevisiae - enzymology
Saccharomyces cerevisiae - genetics
Software
ISSN:0022-2844
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Shrnutí:The comparison of the amino acid sequences of four yeast transport proteins indicates that there is a questionable relatedness between the uracil permease (FUR4) and the purine-cytosine permease (FCY2), whereas the arginine permease (CAN1) and the histidine permease (HIP1) clearly originated from a common molecular ancestor. The analysis of the primary structure of these transport proteins by two methods of secondary structure predictions suggests the presence of 9-12 membrane-spanning alpha-helices in each polypeptide chain. These results are concordant in that 90% of the alpha-helices were determined by both methods to be at the same positions. In the aligned sequences HIP1 and CAN1, the postulated membrane-spanning alpha-helices often start at corresponding sites, even though the overall sequence similarity of the two proteins is only 30%. In the aligned DNA coding sequences of CAN1 and HIP1, synonymous nucleotide substitutions occur with very similar frequencies in regions where the replacement substitution (changing the amino acids) frequencies are widely different. Moreover, our data suggest that the replacement substitutions can be considered as neutral in the N-terminal segment, whereas the other regions are subject to a conservative selective pressure because, if compared to a random drift, the replacement substitutions are underrepresented.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:0022-2844
DOI:10.1007/BF02101197