A single-cell atlas of the peripheral immune response to severe COVID-19

There is an urgent need to better understand the pathophysiology of Coronavirus disease 2019 (COVID-19), the global pandemic caused by SARS-CoV-2. Here, we apply single-cell RNA sequencing (scRNA-seq) to peripheral blood mononuclear cells (PBMCs) of 7 patients hospitalized with confirmed COVID-19 an...

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Veröffentlicht in:	medRxiv : the preprint server for health sciences
Hauptverfasser:	Wilk, Aaron J, Rustagi, Arjun, Zhao, Nancy Q, Roque, Jonasel, Martinez-Colon, Giovanny J, McKechnie, Julia L, Ivison, Geoffrey T, Ranganath, Thanmayi, Vergara, Rosemary, Hollis, Taylor, Simpson, Laura J, Grant, Philip, Subramanian, Aruna, Rogers, Angela J, Blish, Catherine A
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	United States 23.04.2020
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Zusammenfassung:	There is an urgent need to better understand the pathophysiology of Coronavirus disease 2019 (COVID-19), the global pandemic caused by SARS-CoV-2. Here, we apply single-cell RNA sequencing (scRNA-seq) to peripheral blood mononuclear cells (PBMCs) of 7 patients hospitalized with confirmed COVID-19 and 6 healthy controls. We identify substantial reconfiguration of peripheral immune cell phenotype in COVID-19, including a heterogeneous interferon-stimulated gene (ISG) signature, HLA class II downregulation, and a novel B cell-derived granulocyte population appearing in patients with acute respiratory failure requiring mechanical ventilation. Importantly, peripheral monocytes and lymphocytes do not express substantial amounts of pro-inflammatory cytokines, suggesting that circulating leukocytes do not significantly contribute to the potential COVID-19 cytokine storm. Collectively, we provide the most thorough cell atlas to date of the peripheral immune response to severe COVID-19.
Bibliographie:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Working Paper/Pre-Print-1 ObjectType-Feature-3 content type line 23
DOI:	10.1101/2020.04.17.20069930