The identification and developmental requirements of colonic CD169+ macrophages

Summary CD169‐positive macrophages in the marginal zone of the spleen and subcapsular sinus of lymph nodes play an important role as gatekeepers, strategically located to capture pathogens. Here we identified a population of CD169‐positive macrophages in the colon and investigated which factors infl...

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Published in:Immunology Vol. 142; no. 2; pp. 269 - 278
Main Authors: Hiemstra, Ida H., Beijer, Marieke R., Veninga, Henrike, Vrijland, Kim, Borg, Ellen G. F., Olivier, Brenda J., Mebius, Reina E., Kraal, Georg, Haan, Joke M. M.
Format: Journal Article
Language:English
Published: England Wiley Subscription Services, Inc 01.06.2014
John Wiley & Sons Ltd
Subjects:
Animals
Colon - cytology
Colon - immunology
Colon - microbiology
development
Female
Flora
intestine
Lymph nodes
Lymphotoxin-alpha
Macrophages - cytology
Macrophages - immunology
Mice
Mice, Inbred C57BL
Mice, Knockout
Mucous Membrane - cytology
Mucous Membrane - immunology
Myelopoiesis
Sialic Acid Binding Ig-like Lectin 1 - immunology
Siglec‐1
spleen
vitamin A
Vitamin A - metabolism
ISSN:0019-2805, 1365-2567, 1365-2567
Online Access:Get full text
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Summary:Summary CD169‐positive macrophages in the marginal zone of the spleen and subcapsular sinus of lymph nodes play an important role as gatekeepers, strategically located to capture pathogens. Here we identified a population of CD169‐positive macrophages in the colon and investigated which factors influenced their development. Murine colonic CD115+ F4/80lo CD11clo macrophages expressing CD169 were present in the lamina propria, mainly surrounding the crypts. In spite of the high levels of bacterial flora in the colon and the importance of Toll‐like receptor signalling in mucosal homeostasis, the presence of CD169+ macrophages was not affected in mice that were deficient in MyD88‐mediated Toll‐like receptor signalling and in mice in which the bacterial flora was eradicated. Whereas the development of splenic CD169+ macrophages was dependent on lymphotoxin α, colonic CD169+ macrophages were present in normal numbers in lymphotoxin α‐deficient mice. In contrast, reduced numbers of CD169+ macrophages were found in the colon of mice deficient in vitamin A, whereas CD169+ macrophages in the spleen were unaffected. In conclusion, we identified a new macrophage subset in the lamina propria of the colon characterized by the expression of CD169. Its differentiation, unlike CD169+ macrophages in lymphoid organs, is independent of lymphotoxin α signalling, but requires vitamin A.
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Senior author: Joke M. M. den Haan
ISSN:0019-2805
1365-2567
1365-2567
DOI:10.1111/imm.12251