Puccinia striiformis f. sp. tritici microRNA-like RNA 1 (Pst-milR1), an important pathogenicity factor of Pst, impairs wheat resistance to Pst by suppressing the wheat pathogenesis-related 2 gene

Small RNAs (sRNAs), an important type of pathogenicity factor, contribute to impairing host immune responses. However, little is known about sRNAs in Puccinia striiformis f. sp. tritici (Pst), one of the most destructive pathogens of wheat (Triticum aestivum L.). Here, we report a novel microRNA-lik...

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Vydané v:The New phytologist Ročník 215; číslo 1; s. 338 - 350
Hlavní autori: Wang, Bing, Sun, Yanfei, Song, Na, Zhao, Mengxin, Liu, Rui, Feng, Hao, Wang, Xiaojie, Kang, Zhensheng
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: England New Phytologist Trust 01.07.2017
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Predmet:
Basidiomycota - pathogenicity
Binding
Defence mechanisms
Disease Resistance
Gene expression
Genes
Genetic transformation
host‐induced gene silencing (HIGS)
Identification
Immune response
Immune system
Immunity
Interactions
Interference
MicroRNAs
MicroRNAs - genetics
MicroRNAs - metabolism
MicroRNAs - physiology
miRNA
Nucleic acids
Pathogenesis
pathogenesis‐related (PR) gene
Pathogenicity
Pathogens
Plant Diseases - microbiology
Plant immunity
Plants (botany)
prediction
Pst Dicer‐like protein (PsDCL)
Puccinia striiformis (Pst)
Puccinia striiformis f. sp. tritici microRNA‐like RNA 1 (Pst‐milR1)
Puccinia striiformis f. tritici
Retarding
Ribonucleic acid
RNA
RNA interference
RNA-mediated interference
Rusting
Stripe rust
Triticum - microbiology
Triticum aestivum
virulence
Virulence - genetics
Wheat
pathogenesis-related (PR) gene
Puccinia striiformis f. sp. tritici microRNA-like RNA 1 (Pst-milR1)
Puccinia striiformis (Pst)
host-induced gene silencing (HIGS)
Pst Dicer-like protein (PsDCL)
ISSN:0028-646X, 1469-8137, 1469-8137
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Shrnutí:Small RNAs (sRNAs), an important type of pathogenicity factor, contribute to impairing host immune responses. However, little is known about sRNAs in Puccinia striiformis f. sp. tritici (Pst), one of the most destructive pathogens of wheat (Triticum aestivum L.). Here, we report a novel microRNA-like RNA (milRNA) from Pst termed microRNA-like RNA 1 (Pst milR1), which suppresses wheat defenses during wheat–Pst interactions. We identified Pst-milR1 as a novel milRNA in Pst. Biological prediction and co-transformation showed that Pst-milR1 takes part in cross-kingdom RNA interference (RNAi) events by binding the wheat pathogenesis-related 2 (PR2) gene. Silencing of the Pst-milR1 precursor resulted in increased wheat resistance to the virulent Pst isolate CYR31. PR2 knock-down plants increased the susceptibility of wheat to the avirulent Pst isolate CYR23. This suggests that Pst-milR1 represses the plant immune response by suppressing the expression of PR2. Taking our findings together, we postulate that Pst-milR1 is an important pathogenicity factor in Pst, which acts as an effector to suppress host immunity. Our results provide significant new insights into the pathogenicity of the stripe rust pathogen.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0028-646X
1469-8137
1469-8137
DOI:10.1111/nph.14577