Absence of vertical transmission of infectious salmon anemia virus (ISAV) from individually infected Atlantic salmon Salmo salar

Atlantic salmon Salmo salar L. eggs were collected from grilse that were individually identified as ISAV-positive based on the detection of pathogen in ovarian fluid by RT-PCR. The eggs were fertilised, disinfected and reared under quarantine conditions. To address the possibility of vertical transm...

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Bibliographic Details
Published in:Diseases of aquatic organisms Vol. 38; no. 3; p. 231
Main Authors: Melville, K J, Griffiths, S G
Format: Journal Article
Language:English
Published: Germany 30.11.1999
Subjects:
Anemia - veterinary
Anemia - virology
Animals
DNA Primers - chemistry
DNA, Viral - chemistry
Female
Fish Diseases - transmission
Fish Diseases - virology
Infectious Disease Transmission, Vertical - veterinary
Male
New Brunswick
Orthomyxoviridae - growth & development
Orthomyxoviridae Infections - transmission
Orthomyxoviridae Infections - veterinary
Orthomyxoviridae Infections - virology
Reverse Transcriptase Polymerase Chain Reaction - veterinary
RNA, Viral - chemistry
RNA, Viral - isolation & purification
Salmo salar
New Brunswick
ISSN:0177-5103
Online Access:Get more information
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Summary:Atlantic salmon Salmo salar L. eggs were collected from grilse that were individually identified as ISAV-positive based on the detection of pathogen in ovarian fluid by RT-PCR. The eggs were fertilised, disinfected and reared under quarantine conditions. To address the possibility of vertical transmission, fertilised eggs, alevins and parr were screened for the virus by SHK-1 cell culture and RT-PCR. In addition, ISAV-negative parr were injected with homogenates of potentially infected eyed eggs. ISAV was not detected in eyed eggs, alevins or parr. No mortalities occurred among fish injected with the egg homogenates. These observations suggest the absence of a vertical transmission route for ISAV infection.
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ISSN:0177-5103
DOI:10.3354/dao038231