Metabolite profiling reveals temperature effects on the VOCs and flavonoids of different plant populations
Temperature is one of the key factors in limiting the distribution of plants and controlling major metabolic processes. A series of simulated reciprocal transplant experiments were performed to investigate the effect of temperature on plant chemical composition. Polygonum minus of different lowland...
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| Vydáno v: | Plant biology (Stuttgart, Germany) Ročník 18; číslo S1; s. 130 - 139 |
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| Hlavní autoři: | , , , , |
| Médium: | Journal Article |
| Jazyk: | angličtina |
| Vydáno: |
England
Blackwell Publishing Ltd
01.01.2016
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| Témata: | |
| ISSN: | 1435-8603, 1438-8677, 1438-8677 |
| On-line přístup: | Získat plný text |
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| Shrnutí: | Temperature is one of the key factors in limiting the distribution of plants and controlling major metabolic processes. A series of simulated reciprocal transplant experiments were performed to investigate the effect of temperature on plant chemical composition. Polygonum minus of different lowland and highland origin were grown under a controlled environment with different temperature regimes to study the effects on secondary metabolites. We applied gas chromatography–mass spectrometry and liquid chromatography time‐of‐flight mass spectrometry to identify the chemical compounds. A total of 37 volatile organic compounds and 85 flavonoids were detected, with the largest response observed in the compositional changes of aldehydes and terpenes in highland plants under higher temperature treatment. Significantly less anthocyanidin compounds and larger amounts of flavonols were detected under higher temperature treatment. We also studied natural variation in the different plant populations growing under the same environment and identified compounds unique to each population through metabolite fingerprinting. This study shows that the origin of different plant populations influences the effects of temperature on chemical composition. |
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| Bibliografie: | Centre for Research and Instrumentation Management Fundamental Research Grant Scheme - No. UKM-RB-06-FRGS0102-2009 Genomics and Molecular Biology Initiative of the Malaysia Genome Institute - No. 07-05-MGI-GMB 004 Arus Perdana - No. UKM-AP-BPB-14-2009 ark:/67375/WNG-TFWR2GH7-3 ArticleID:PLB12403 Figure S1. PCA score plots of P. minus samples based on volatile compounds grouped according to (a) temperature treatments and (b) different populations.Figure S2. PCA score plots of P. minus samples based on flavonoids grouped according to (a) temperature treatments and (b) different populations.Figure S3. Heatmap of relative abundance of different flavonoids in P. minus grown under baseline, lower and higher temperature treatments.Figure S4. PCA score plots of P. minus samples based on combined datasets of VOCs and flavonoids grouped according to (a) temperature treatments and (b) different populations.Figure S5. PLS-DA score plots of P. minus samples based on combined datasets of VOCs and flavonoids according to (a) temperature treatments and (b) different populations.Table S1. Identification of volatile compounds from P. minus based on the retention index.Table S2. List of putatively identified flavonoid compounds from P. minus categorised based on different classes of flavonoids.Table S3. List of unique compounds identified from different populations of P. minus. Universiti Kebangsaan Malaysia for Research Instrumentation Development Grant 2010 (PIP-CRIM). istex:82BB10EA8D1C29E4F0786FBBBB03AA146F55BCDE ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| ISSN: | 1435-8603 1438-8677 1438-8677 |
| DOI: | 10.1111/plb.12403 |