Histopathological characterization of primary cutaneous melanoma using infrared microimaging: a proof-of-concept study

Summary Background  The diagnosis of malignant melanoma is based upon the histological evaluation of the lesion. As such, the morphological interpretation relies on the expertise of a dermatopathologist. Infrared microimaging is emerging as a new powerful tool to investigate tissue biochemistry. Inf...

Celý popis

Uložené v:
Podrobná bibliografia
Vydané v:British journal of dermatology (1951) Ročník 162; číslo 6; s. 1316 - 1323
Hlavní autori: Ly, E., Cardot-Leccia, N., Ortonne, J.-P., Benchetrit, M., Michiels, J.-F., Manfait, M., Piot, O.
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: Oxford, UK Blackwell Publishing Ltd 01.06.2010
Wiley-Blackwell
Wiley
Predmet:
Adult
Aged
Aged, 80 and over
Biological and medical sciences
cluster analysis
Dermatology
Female
Fourier Transform Infrared Spectroscopy
Humans
Infrared Rays
Male
Medical sciences
melanoma
Melanoma - diagnosis
Melanoma - pathology
Middle Aged
Multivariate Analysis
Skin Neoplasms - diagnosis
Skin Neoplasms - pathology
Spectroscopy, Fourier Transform Infrared - methods
Tumors of the skin and soft tissue. Premalignant lesions
Dermatology
Fourier Transform Infrared Spectroscopy
Malignant melanoma
melanoma
Skin
Malignant tumor
cluster analysis
Cancer
ISSN:0007-0963, 1365-2133, 1365-2133
On-line prístup:Získať plný text
Tagy: Pridať tag
Žiadne tagy, Buďte prvý, kto otaguje tento záznam!
Popis
Shrnutí:Summary Background  The diagnosis of malignant melanoma is based upon the histological evaluation of the lesion. As such, the morphological interpretation relies on the expertise of a dermatopathologist. Infrared microimaging is emerging as a new powerful tool to investigate tissue biochemistry. Infrared spectra probe the biochemical constitution of the sample and are real tissue‐specific spectroscopic fingerprints. Objectives  To assess the potential of infrared microimaging to aid in the analysis of tissue sections from primary cutaneous melanomas. Methods  Ten samples of melanoma sections from the main histological subtypes were investigated using infrared microimaging combined with multivariate statistical analyses. Results  This methodology yielded highly contrasted colour‐coded images that permitted to highlight tissue architecture without any staining. It was possible to discriminate tumour areas from normal epidermis automatically, and intratumoral heterogeneity as revealed by our approach was correlated with the aggressiveness of the tumour. Conclusions  This proof‐of‐concept study shows that infrared microimaging could help in the diagnosis of primary cutaneous melanoma.
Bibliografia:istex:096493CCDB7227D08F8DD126690C4646225A119A
ArticleID:BJD9762
ark:/67375/WNG-BGFBSMHN-D
Conflicts of interest
None declared.
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0007-0963
1365-2133
1365-2133
DOI:10.1111/j.1365-2133.2010.09762.x