Methods for Detection of NOX-Derived Superoxide Radical Anion and Hydrogen Peroxide in Cells
NADPH oxidases (NOX) are transmembrane enzymes, which catalyze the formation of reactive oxygen species (ROS). In humans and most mammals, the NOX family comprises seven members, namely, NOX1-5 and the dual oxidases DUOX1 and 2. The primary product of most NOX isoforms is the superoxide radical anio...
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| Vydané v: | Methods in molecular biology (Clifton, N.J.) Ročník 1982; s. 233 |
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| Hlavní autori: | , , |
| Médium: | Journal Article |
| Jazyk: | English |
| Vydavateľské údaje: |
United States
2019
|
| Predmet: | |
| ISSN: | 1940-6029, 1940-6029 |
| On-line prístup: | Zistit podrobnosti o prístupe |
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| Shrnutí: | NADPH oxidases (NOX) are transmembrane enzymes, which catalyze the formation of reactive oxygen species (ROS). In humans and most mammals, the NOX family comprises seven members, namely, NOX1-5 and the dual oxidases DUOX1 and 2. The primary product of most NOX isoforms is the superoxide radical anion O
, which is rapidly dismutated in hydrogen peroxide (H
O
), while NOX4 and DUOX mostly generate H
O
ROS are multifunctional molecules in tissues, and NOX-derived ROS cellular functions are as diverse as microbial killing (NOX2), thyroid hormone synthesis (DUOX2), or otoconia formation in the inner ear (NOX3). NOX are potential pharmacological targets in numerous diseases such as diabetes, fibrosis, and brain ischemia, and NOX inhibitors are currently under development. Here we describe two cellular assays to detect extracellular O
and H
O
in cells overexpressing specific NOX isoforms and their subunits. |
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| Bibliografia: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| ISSN: | 1940-6029 1940-6029 |
| DOI: | 10.1007/978-1-4939-9424-3_13 |