Novel Giant Phages vB_(A)erVM₃32-Vera and vB_(A)erVM₃32-Igor and Siphophage vB_(A)erVS₃32-Yulya Infecting the Same Aeromonas veronii Strain

Three novel Aeromonas phages vB_AerVS_332-Yuliya, vB_AerVM_332-Vera, and vB_AerVM_332-Igor and their host Aeromonas veronii CEMTC7594 were found in the same water + sediments sample collected in a freshwater pond. Complete genome sequencing indicated that vB_AerVS_332-Yuliya (43,584 bp) is a siphoph...

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Bibliographic Details
Published in:Viruses Vol. 17; no. 8
Main Authors: Babkin, Igor V, Morozova, Vera V, Kozlova, Yuliya N, Fedorets, Valeria A, Tikunov, Artem Y, Ushakova, Tatyana A, Bardasheva, Alevtina V, Zhirakovskaya, Elena V, Tikunova, Nina V
Format: Journal Article
Language:English
Published: MDPI AG 01.08.2025
MDPI
Subjects:
Aeromonas
Bacteriophages
Environmental aspects
Genetic aspects
Physiological aspects
Russia
ISSN:1999-4915, 1999-4915
Online Access:Get full text
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Summary:Three novel Aeromonas phages vB_AerVS_332-Yuliya, vB_AerVM_332-Vera, and vB_AerVM_332-Igor and their host Aeromonas veronii CEMTC7594 were found in the same water + sediments sample collected in a freshwater pond. Complete genome sequencing indicated that vB_AerVS_332-Yuliya (43,584 bp) is a siphophage, whereas vB_AerVM_332-Vera (294,685 bp) and vB_AerVM_332-Igor (237,907 bp) are giant phages. The host strain can grow at temperatures from 5 °C to 37 °C with an optimum of 25–37 °C; siphophage vB_AerVS_332-Yuliya effectively reproduced at temperature ≤ 25 °C, the optimal temperature for giant phage vB_AerVM_332-Igor was 25 °C, and giant phage vB_AerVM_332-Vera infected host cells at 5–10 °C. The genomes of these phages differed significantly from known phages; their level of nucleotide identity and values of intergenomic similarity with the corresponding neighboring phages indicated that each of these phages is a member of a new genus/subfamily. Giant phage vB_AerVM_332-Vera is a member of the proposed Chimallinviridae family, which forms Cluster D of giant phages that possibly evolved from phages with shorter genomes. Giant phage vB_AerVM_332-Igor is part of Cluster E, the known members of which preserve the size of genomes. Phages from Cluster F, containing Aeromonas phages among others, show a gradual decrease and/or increase in genomes during evolution, which indicates different strategies for giant phages.
ISSN:1999-4915
1999-4915
DOI:10.3390/v17081027