A multiplexed chip-based assay system for investigating the functional development of human skeletal myotubes in vitro

•An assay for measuring functional performance of human muscle fibers was developed.•The system provides multiple data points per chip in defined serum-free conditions.•The collected data characterizes the maturation of human muscle cells in vitro.•This system may prove useful for future pre-clinica...

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Vydané v:	Journal of biotechnology Ročník 185; s. 15 - 18
Hlavní autori:	Smith, A.S.T., Long, C.J., Pirozzi, K., Najjar, S., McAleer, C., Vandenburgh, H.H., Hickman, J.J.
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	Netherlands Elsevier B.V 20.09.2014
Predmet:	Assaying atomic force microscopy Biotechnology Body-on-a-chip Cantilevers Cell Culture Techniques - methods Culture Drug Discovery - methods drugs Functional assay High-Throughput Screening Assays - methods Human Humans in vitro studies In vitro testing Microchip Analytical Procedures - methods Microscopy, Atomic Force - methods Multiplexing Muscle Fibers, Skeletal - physiology Muscle, Skeletal - growth & development Muscles myotubes silicon Skeletal muscle Survival Human Cantilevers DETA Body-on-a-chip Functional assay Skeletal muscle
ISSN:	0168-1656, 1873-4863, 1873-4863
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Abstract	•An assay for measuring functional performance of human muscle fibers was developed.•The system provides multiple data points per chip in defined serum-free conditions.•The collected data characterizes the maturation of human muscle cells in vitro.•This system may prove useful for future pre-clinical drug studies.•This is the first chip-based assay for drug discovery using human skeletal muscle. This report details the development of a non-invasive in vitro assay system for investigating the functional maturation and performance of human skeletal myotubes. Data is presented demonstrating the survival and differentiation of human myotubes on microscale silicon cantilevers in a defined, serum-free system. These cultures can be stimulated electrically and the resulting contraction quantified using modified atomic force microscopy technology. This system provides a higher degree of sensitivity for investigating contractile waveforms than video-based analysis, and represents the first system capable of measuring the contractile activity of individual human muscle myotubes in a reliable, high-throughput and non-invasive manner. The development of such a technique is critical for the advancement of body-on-a-chip platforms toward application in pre-clinical drug development screens.
AbstractList	•An assay for measuring functional performance of human muscle fibers was developed.•The system provides multiple data points per chip in defined serum-free conditions.•The collected data characterizes the maturation of human muscle cells in vitro.•This system may prove useful for future pre-clinical drug studies.•This is the first chip-based assay for drug discovery using human skeletal muscle. This report details the development of a non-invasive in vitro assay system for investigating the functional maturation and performance of human skeletal myotubes. Data is presented demonstrating the survival and differentiation of human myotubes on microscale silicon cantilevers in a defined, serum-free system. These cultures can be stimulated electrically and the resulting contraction quantified using modified atomic force microscopy technology. This system provides a higher degree of sensitivity for investigating contractile waveforms than video-based analysis, and represents the first system capable of measuring the contractile activity of individual human muscle myotubes in a reliable, high-throughput and non-invasive manner. The development of such a technique is critical for the advancement of body-on-a-chip platforms toward application in pre-clinical drug development screens. This report details the development of a non-invasive in vitro assay system for investigating the functional maturation and performance of human skeletal myotubes. Data is presented demonstrating the survival and differentiation of human myotubes on microscale silicon cantilevers in a defined, serum-free system. These cultures can be stimulated electrically and the resulting contraction quantified using modified atomic force microscopy technology. This system provides a higher degree of sensitivity for investigating contractile waveforms than video-based analysis, and represents the first system capable of measuring the contractile activity of individual human muscle myotubes in a reliable, high-throughput and non-invasive manner. The development of such a technique is critical for the advancement of body-on-a-chip platforms toward application in pre-clinical drug development screens. This report details the development of a non-invasive in vitro assay system for investigating the functional maturation and performance of human skeletal myotubes. Data is presented demonstrating the survival and differentiation of human myotubes on microscale silicon cantilevers in a defined, serum-free system. These cultures can be stimulated electrically and the resulting contraction quantified using modified atomic force microscopy technology. This system provides a higher degree of sensitivity for investigating contractile waveforms than video-based analysis, and represents the first system capable of measuring the contractile activity of individual human muscle myotubes in a reliable, high-throughput and non-invasive manner. The development of such a technique is critical for the advancement of body-on-a-chip platforms toward application in pre-clinical drug development screens.This report details the development of a non-invasive in vitro assay system for investigating the functional maturation and performance of human skeletal myotubes. Data is presented demonstrating the survival and differentiation of human myotubes on microscale silicon cantilevers in a defined, serum-free system. These cultures can be stimulated electrically and the resulting contraction quantified using modified atomic force microscopy technology. This system provides a higher degree of sensitivity for investigating contractile waveforms than video-based analysis, and represents the first system capable of measuring the contractile activity of individual human muscle myotubes in a reliable, high-throughput and non-invasive manner. The development of such a technique is critical for the advancement of body-on-a-chip platforms toward application in pre-clinical drug development screens. This report details the development of a non-invasive in vitro assay system for investigating the functional maturation and performance of human skeletal myotubes. Data is presented demonstrating the survival and differentiation of human myotubes on microscale silicon cantilevers in a defined, serum-free system. These cultures can be stimulated electrically and the resulting contraction quantified using modified atomic force microscopy technology. This system provides a higher degree of sensitivity for investigating contractile waveforms than video-based analysis, and represents the first system capable of measuring the contractile activity of individual human muscle myotubes in a reliable, high-throughput and non-invasive manner. The development of such a technique is critical for the advancement of body-on-a-chip platforms towards application in future pre-clinical drug development screens.
Author	Vandenburgh, H.H. Najjar, S. McAleer, C. Hickman, J.J. Long, C.J. Pirozzi, K. Smith, A.S.T.
AuthorAffiliation	b Department of Pathology and Laboratory Medicine, Brown University, Providence, RI 02906, USA a NanoScience Technology Center, University of Central Florida, 12424 Research Parkway, Orlando, FL 32826, USA
AuthorAffiliation_xml	– name: b Department of Pathology and Laboratory Medicine, Brown University, Providence, RI 02906, USA – name: a NanoScience Technology Center, University of Central Florida, 12424 Research Parkway, Orlando, FL 32826, USA
Author_xml	– sequence: 1 givenname: A.S.T. surname: Smith fullname: Smith, A.S.T. organization: NanoScience Technology Center, University of Central Florida, 12424 Research Parkway, Orlando, FL 32826, USA – sequence: 2 givenname: C.J. surname: Long fullname: Long, C.J. organization: NanoScience Technology Center, University of Central Florida, 12424 Research Parkway, Orlando, FL 32826, USA – sequence: 3 givenname: K. surname: Pirozzi fullname: Pirozzi, K. organization: NanoScience Technology Center, University of Central Florida, 12424 Research Parkway, Orlando, FL 32826, USA – sequence: 4 givenname: S. surname: Najjar fullname: Najjar, S. organization: NanoScience Technology Center, University of Central Florida, 12424 Research Parkway, Orlando, FL 32826, USA – sequence: 5 givenname: C. surname: McAleer fullname: McAleer, C. organization: NanoScience Technology Center, University of Central Florida, 12424 Research Parkway, Orlando, FL 32826, USA – sequence: 6 givenname: H.H. surname: Vandenburgh fullname: Vandenburgh, H.H. organization: Department of Pathology and Laboratory Medicine, Brown University, Providence, RI 02906, USA – sequence: 7 givenname: J.J. surname: Hickman fullname: Hickman, J.J. email: jhickman@mail.ucf.edu organization: NanoScience Technology Center, University of Central Florida, 12424 Research Parkway, Orlando, FL 32826, USA
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Keywords	Human Cantilevers DETA Body-on-a-chip Functional assay Skeletal muscle
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Snippet	•An assay for measuring functional performance of human muscle fibers was developed.•The system provides multiple data points per chip in defined serum-free... This report details the development of a non-invasive in vitro assay system for investigating the functional maturation and performance of human skeletal...
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SubjectTerms	Assaying atomic force microscopy Biotechnology Body-on-a-chip Cantilevers Cell Culture Techniques - methods Culture Drug Discovery - methods drugs Functional assay High-Throughput Screening Assays - methods Human Humans in vitro studies In vitro testing Microchip Analytical Procedures - methods Microscopy, Atomic Force - methods Multiplexing Muscle Fibers, Skeletal - physiology Muscle, Skeletal - growth & development Muscles myotubes silicon Skeletal muscle Survival
Title	A multiplexed chip-based assay system for investigating the functional development of human skeletal myotubes in vitro
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