Optimization of ion-exchange displacement separations: I. Validation of an iterative scheme and its use as a methods development tool

Displacement chromatography has been demonstrated to be a powerful, high-resolution preparative tool. The performance of displacement systems can be affected by a variety of factors such as the feed load, flow-rate, initial salt concentration and the displacer partition ratio. Thus, the optimization...

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Bibliographic Details
Published in:Journal of Chromatography A Vol. 876; no. 1; pp. 51 - 62
Main Authors: Natarajan, Venkatesh, Wayne Bequette, B., Cramer, Steven M.
Format: Journal Article
Language:English
Published: Amsterdam Elsevier B.V 21.04.2000
Elsevier
Subjects:
Algorithms
Analytical chemistry
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Chemistry
Chromatographic methods and physical methods associated with chromatography
Chromatography, Ion Exchange - methods
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
General aspects, investigation methods
Other chromatographic methods
Proteins
Proteins - analysis
Quality Control
Reproducibility of Results
Salts - chemistry
Displacement chromatography
Steric mass action
Ion-exchange chromatography
Optimization
Hemoprotein
Phase composition
Theoretical study
Mobile phase
Iterative program
Displacement
Protein
Mass transfer
Ion exchange chromatography
Operating conditions
Cytochrome c
Salt effect
Ion exchanger
Numerical simulation
ISSN:0021-9673
Online Access:Get full text
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Summary:Displacement chromatography has been demonstrated to be a powerful, high-resolution preparative tool. The performance of displacement systems can be affected by a variety of factors such as the feed load, flow-rate, initial salt concentration and the displacer partition ratio. Thus, the optimization of displacement separations is a uniquely challenging problem. In this manuscript, an iterative optimization scheme has been presented whereby one can identify the optimum operating conditions for displacement separations at a given level of loading on a given resin material. The solid film linear driving force model has been employed in concert with the Steric Mass Action formalism of ion-exchange chromatography to describe the chromatographic behavior in these systems. Simple pulse techniques have been employed to estimate the transport parameters. The iterative scheme has been validated using a rigorous Feasible Sequential Quadratic Programming algorithm. Finally, the utility of the iterative optimization scheme as a methods development tool for displacement separations has been demonstrated for a difficult separation. The results indicate that the use of the optimization scheme leads to significantly better performance than standard rules of thumb.
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ISSN:0021-9673
DOI:10.1016/S0021-9673(00)00138-2