Transcriptome atlases of rat brain regions and their adaptation to diabetes resolution following gastrectomy in the Goto-Kakizaki rat

Brain regions drive multiple physiological functions through specific gene expression patterns that adapt to environmental influences, drug treatments and disease conditions. To generate a detailed atlas of the brain transcriptome in the context of diabetes, we carried out RNA sequencing in hypothal...

Celý popis

Uloženo v:
Podrobná bibliografie
Vydáno v:Molecular brain Ročník 18; číslo 1; s. 9 - 15
Hlavní autoři: Brial, François, Le Lay, Aurélie, Rouch, Claude, Henrion, Edouard, Bourgey, Mathieu, Bourque, Guillaume, Lathrop, Mark, Magnan, Christophe, Gauguier, Dominique
Médium: Journal Article
Jazyk:angličtina
Vydáno: London BioMed Central 07.02.2025
BioMed Central Ltd
BMC
Témata:
Adaptation, Physiological - genetics
Animals
Anopheles
Atlases as Topic
Bariatric surgery
Bibliography
Biomedical and Life Sciences
Biomedicine
Brain
Brain - metabolism
Brain - pathology
Diabetes Mellitus, Type 2 - genetics
Diabetes Mellitus, Type 2 - surgery
Diabetes therapy
Gastrectomy
Gene Expression Profiling
Gene Expression Regulation
Genes
Genetic aspects
Genetic transcription
Life Sciences
Male
Neurology
Neurophysiology
Neurosciences
Obesity
Physiological aspects
Psychopharmacology
Rats
RNA
RNA sequencing
Surgery
Transcriptome - genetics
Type 2 diabetes
Vertical sleeve gastrectomy
France
RNA sequencing
Vertical sleeve gastrectomy
Bariatric surgery
ISSN:1756-6606, 1756-6606
On-line přístup:Získat plný text
Tagy: Přidat tag
Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!
Popis
Shrnutí:Brain regions drive multiple physiological functions through specific gene expression patterns that adapt to environmental influences, drug treatments and disease conditions. To generate a detailed atlas of the brain transcriptome in the context of diabetes, we carried out RNA sequencing in hypothalamus, hippocampus, brainstem and striatum of the Goto-Kakizaki (GK) rat model of spontaneous type 2 diabetes, which was applied to identify gene transcription adaptation to improved glycemic control following vertical sleeve gastrectomy (VSG) in the GK. Over 19,000 distinct transcripts were detected in the rat brain, including 2794 which were consistently expressed in the four brain regions. Region-specific gene expression was identified in hypothalamus (n = 477), hippocampus (n = 468), brainstem (n = 1173) and striatum (n = 791), resulting in differential regulation of biological processes between regions. Differentially expressed genes between VSG and sham operated rats were only found in the hypothalamus and were predominantly involved in the regulation of endothelium and extracellular matrix. These results provide a detailed atlas of regional gene expression in the diabetic rat brain and suggest that the long term effects of gastrectomy-promoted diabetes remission involve functional changes in the hypothalamus endothelium.
Bibliografie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1756-6606
1756-6606
DOI:10.1186/s13041-025-01176-z