Dynamic and functional analyses of exosomal miRNAs regulating cellular microenvironment of ovarian cancer cells

Background Exosomes, extracellular vesicles with an average diameter of 30 ~ 150 nm, are pivotal in mediating the cellular microenvironment (CM) through their cargo-carrying capability. Despite extensive studies, the dynamic and regulatory mechanisms of exosomal cargoes, including lipids, proteins,...

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Published in:Journal of ovarian research Vol. 18; no. 1; pp. 25 - 11
Main Authors: Wang, Zhaoxia, Huang, Yanan, He, Simin, Zhou, Ying, Zhao, Le, Wang, Fuyuan
Format: Journal Article
Language:English
Published: London BioMed Central 10.02.2025
BioMed Central Ltd
BMC
Subjects:
Cancer cells
Cell Line, Tumor
Cell organelles
Cellular microenvironment
Exosomes - genetics
Exosomes - metabolism
Exsomes
Female
Gene Expression Regulation, Neoplastic
Genetic aspects
Gynecology
Health aspects
Humans
Medicine
Medicine & Public Health
MicroRNA
MicroRNAs - genetics
MicroRNAs - metabolism
miRNA sequencing
miRNAs
Ovarian cancer
Ovarian Neoplasms - genetics
Ovarian Neoplasms - metabolism
Ovarian Neoplasms - pathology
Physiological aspects
Reproductive Medicine
Tumor Microenvironment - genetics
China
Exsomes
miRNAs
Cellular microenvironment
miRNA sequencing
Ovarian cancer
ISSN:1757-2215, 1757-2215
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Summary:Background Exosomes, extracellular vesicles with an average diameter of 30 ~ 150 nm, are pivotal in mediating the cellular microenvironment (CM) through their cargo-carrying capability. Despite extensive studies, the dynamic and regulatory mechanisms of exosomal cargoes, including lipids, proteins, nucleic acids, and metabolites, remain poorly understood. Methods In this study, we collected culture medium of ovarian cancer cells at four different time points (12, 24, 36, 48 h). Exosomes were isolated using ultracentrifugation, and miRNA sequencing was performed for exosomes from each group (T12, T24, T36, and T48). Results A total of 131 miRNAs were identified in all groups. Specifically, 41, 115, 63, and 24 miRNAs were detected in the T12, T24, T36, and T48 groups, respectively. Among these, 15 miRNAs were common to the all groups, while 3, 57, 10, and 3 miRNAs were unique to the T12, T24, T36, and T48 groups, respectively. Functional analyses of the target genes for both common and specific miRNAs indicated that numerous target genes were involved in signaling pathways and cancer-related processes. Conclusion It suggested that exosomal miRNAs might be critical in intercellular communication and in dynamically remodeling the tumor microenvironment. These insights could enhance our understanding of the role of exosomal miRNAs in cancer biology and inform the development of novel therapeutic strategies.
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ISSN:1757-2215
1757-2215
DOI:10.1186/s13048-025-01608-3