Dynamic and functional analyses of exosomal miRNAs regulating cellular microenvironment of ovarian cancer cells

Background Exosomes, extracellular vesicles with an average diameter of 30 ~ 150 nm, are pivotal in mediating the cellular microenvironment (CM) through their cargo-carrying capability. Despite extensive studies, the dynamic and regulatory mechanisms of exosomal cargoes, including lipids, proteins,...

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Vydáno v:	Journal of ovarian research Ročník 18; číslo 1; s. 25 - 11
Hlavní autoři:	Wang, Zhaoxia, Huang, Yanan, He, Simin, Zhou, Ying, Zhao, Le, Wang, Fuyuan
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	London BioMed Central 10.02.2025 BioMed Central Ltd BMC
Témata:	Cancer cells Cell Line, Tumor Cell organelles Cellular microenvironment Exosomes - genetics Exosomes - metabolism Exsomes Female Gene Expression Regulation, Neoplastic Genetic aspects Gynecology Health aspects Humans Medicine Medicine & Public Health MicroRNA MicroRNAs - genetics MicroRNAs - metabolism miRNA sequencing miRNAs Ovarian cancer Ovarian Neoplasms - genetics Ovarian Neoplasms - metabolism Ovarian Neoplasms - pathology Physiological aspects Reproductive Medicine Tumor Microenvironment - genetics China Exsomes miRNAs Cellular microenvironment miRNA sequencing Ovarian cancer
ISSN:	1757-2215, 1757-2215
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Shrnutí:	Background Exosomes, extracellular vesicles with an average diameter of 30 ~ 150 nm, are pivotal in mediating the cellular microenvironment (CM) through their cargo-carrying capability. Despite extensive studies, the dynamic and regulatory mechanisms of exosomal cargoes, including lipids, proteins, nucleic acids, and metabolites, remain poorly understood. Methods In this study, we collected culture medium of ovarian cancer cells at four different time points (12, 24, 36, 48 h). Exosomes were isolated using ultracentrifugation, and miRNA sequencing was performed for exosomes from each group (T12, T24, T36, and T48). Results A total of 131 miRNAs were identified in all groups. Specifically, 41, 115, 63, and 24 miRNAs were detected in the T12, T24, T36, and T48 groups, respectively. Among these, 15 miRNAs were common to the all groups, while 3, 57, 10, and 3 miRNAs were unique to the T12, T24, T36, and T48 groups, respectively. Functional analyses of the target genes for both common and specific miRNAs indicated that numerous target genes were involved in signaling pathways and cancer-related processes. Conclusion It suggested that exosomal miRNAs might be critical in intercellular communication and in dynamically remodeling the tumor microenvironment. These insights could enhance our understanding of the role of exosomal miRNAs in cancer biology and inform the development of novel therapeutic strategies.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1757-2215 1757-2215
DOI:	10.1186/s13048-025-01608-3