Comparison of The Melatonin Preconditioning Efficacy between Bone Marrow and Adipose-Derived Mesenchymal Stem Cells

Mesenchymal stem cells (MSC) from various sources have the potentials to positively affect regenerative medicine. Furthermore, pre-conditioning strategies with different agents could improve the efficacy of cell therapy. This study compares the effects of an anti-inflammatory and antioxidant agent,...

Full description

Saved in:
Bibliographic Details
Published in:Cell journal (Yakhteh) Vol. 20; no. 4; pp. 450 - 458
Main Authors: Rafat, Ali, Mohammadi Roushandeh, Amaneh, Alizadeh, Akram, Hashemi-Firouzi, Nasrin, Golipoor, Zoleikha
Format: Journal Article
Language:English
Published: Iran Royan Institute of Iran 2019
Royan Institute
Royan Institute (ACECR), Tehran
Subjects:
Adipose tissue
Alizarin
Antioxidants
Apoptosis
Biomedical materials
Bone growth
Bone marrow
Bone Marrow Mesenchymal Stem Cells
Cell proliferation
Cell therapy
Cell viability
Differentiation (biology)
Effectiveness
Gene expression
Growth factors
Inflammation
Melatonin
Melatonin receptors
Mesenchymal stem cells
Original
Osteocalcin
Osteogenesis
Oxidative stress
Polymerase chain reaction
Preconditioning
Receptors
Regeneration
Regeneration (physiology)
Regenerative medicine
RNA-directed DNA polymerase
Staining
Stem cell transplantation
Stem cells
Therapeutic applications
Tissue engineering
Iran
Canada
United States > US
Melatonin
Bone Marrow Mesenchymal Stem Cells
Osteogenesis
Apoptosis
ISSN:2228-5806, 2228-5814
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Mesenchymal stem cells (MSC) from various sources have the potentials to positively affect regenerative medicine. Furthermore, pre-conditioning strategies with different agents could improve the efficacy of cell therapy. This study compares the effects of an anti-inflammatory and antioxidant agent, melatonin, on protection of bone marrow-derived MSCs (BMSCs) and adipose tissue-derived MSCs (ADSCs). In this experimental study, rat BMSCs and ADSCs were isolated and expanded. Pre-conditioning was performed with 5 μM melatonin for 24 hours. Cell proliferation and viability were detected by MTT assay. Expression of BAX, BCL2, melatonin receptors and osteocalcin genes were evaluated by reverse transcriptase-polymerase chain reaction (RT-PCR). Also, apoptosis was detected with tunnel assay. Osteogenic differentiation was analyzed using alizarin red staining. No significant increase was found in cell viability between BMSCs and ADSCs after melatonin preconditioning. Following melatonin preconditioning, BAX expression was significantly down-regulated in both ADSCs and BMSCs (P<0.05), with the difference being more significant in ADSCs compared to BMSCs. BCL2 expression was increased significantly in both cell types after preconditioning. Metalothionine 1 and Metalothionine 2 were both upregulated significantly in the two cell types (P<0.05). Melatonin increased osteogenesis capability through increasing osteocalcin expression. However, expression of osteocalcin in BMSCs before and after preconditioning was higher than that in ADSCs. On the other hand, melatonin expression in ADSCs was in higher levels than in BMSCs. Melatonin also improved alizarin red concentration significantly in both BMSCs and ADSCs (P<0.05). Alizarin red staining severity increased significantly in ADSCs after preconditioning compared to BMSCs (P<0.05). Here we have shown that the effects of preconditioning on melatonin expression in ADSCs are higher than those in BMSCs. These findings could be used in adoption of a proper preconditioning protocol based on the sources of MSCs in specific clinical applications, especially in bone regeneration.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
ISSN:2228-5806
2228-5814
DOI:10.22074/cellj.2019.5507