Integrated Metagenomics/Metaproteomics Reveals Human Host-Microbiota Signatures of Crohn's Disease

Crohn's disease (CD) is an inflammatory bowel disease of complex etiology, although dysbiosis of the gut microbiota has been implicated in chronic immune-mediated inflammation associated with CD. Here we combined shotgun metagenomic and metaproteomic approaches to identify potential functional...

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Vydané v:	PloS one Ročník 7; číslo 11; s. e49138
Hlavní autori:	Erickson, Alison R., Cantarel, Brandi L., Lamendella, Regina, Darzi, Youssef, Mongodin, Emmanuel F., Pan, Chongle, Shah, Manesh, Halfvarson, Jonas, Tysk, Curt, Henrissat, Bernard, Raes, Jeroen, Verberkmoes, Nathan C., Fraser, Claire M., Hettich, Robert L., Jansson, Janet K.
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	United States Public Library of Science 28.11.2012 Public Library of Science (PLoS)
Predmet:	Acids Acquired immune deficiency syndrome AIDS Bacteria Bacteria - genetics Bacteria - metabolism Bioengineering Bioindicators Bioinformatics Biology Biomarkers Carbohydrate metabolism Carbohydrates Cluster Analysis Colon Crohn Disease - metabolism Crohn Disease - microbiology Crohn's Disease Crohns disease Development and progression Diagnostic systems Dysbacteriosis Earth science Etiology Female Gastroenterology Gastrointestinal diseases Gastrointestinal Tract - metabolism Gastrointestinal Tract - microbiology Genomes Humans Ileum Ileum - metabolism Ileum - microbiology Ileum - pathology Inflammation Inflammatory bowel disease Inflammatory bowel diseases Internal medicine Intestinal microflora Intestinal Mucosa - metabolism Intestinal Mucosa - microbiology Intestinal Mucosa - pathology Intestine Laboratories Male Mass spectrometry Medicin Medicine Metabolic Networks and Pathways Metabolism Metagenome Metagenomics Microbiota Microbiota (Symbiotic organisms) Proteins Proteome Proteomics Scientific imaging Shotguns Signatures Studies Target recognition Twins, Monozygotic California Belgium United States > US Maryland Tennessee Baltimore Maryland
ISSN:	1932-6203, 1932-6203
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Popis
Shrnutí:	Crohn's disease (CD) is an inflammatory bowel disease of complex etiology, although dysbiosis of the gut microbiota has been implicated in chronic immune-mediated inflammation associated with CD. Here we combined shotgun metagenomic and metaproteomic approaches to identify potential functional signatures of CD in stool samples from six twin pairs that were either healthy, or that had CD in the ileum (ICD) or colon (CCD). Integration of these omics approaches revealed several genes, proteins, and pathways that primarily differentiated ICD from healthy subjects, including depletion of many proteins in ICD. In addition, the ICD phenotype was associated with alterations in bacterial carbohydrate metabolism, bacterial-host interactions, as well as human host-secreted enzymes. This eco-systems biology approach underscores the link between the gut microbiota and functional alterations in the pathophysiology of Crohn's disease and aids in identification of novel diagnostic targets and disease specific biomarkers.
Bibliografia:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 USDOE DE-AC05-00OR22725 Work for Others (WFO) Current address: Juniata College, Biology Department, Huntingdon, Pennsylvania, United States of America Competing Interests: The authors have declared that no competing interests exist. These authors are joint Senior Authors. Conceived and designed the experiments: JKJ CMF RLH. Performed the experiments: JH CT RL BLC EFM ARE NCV. Analyzed the data: ARE BLC CP NCV RLH MS CP YD JR BH BLC. Wrote the paper: ARE BLC RL JKJ CMF RLH.
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0049138