Transmission electron microscopic observation of body cuticle structures of phoretic and parasitic stages of Parasitaphelenchinae nematodes

Using transmission electron microscopy, we examined the body cuticle ultrastructures of phoretic and parasitic stages of the parasitaphelenchid nematodes Bursaphelenchus xylophilus, B. conicaudatus, B. luxuriosae, B. rainulfi; an unidentified Bursaphelenchus species, and an unidentified Parasitaphel...

Celý popis

Uloženo v:

Podrobná bibliografie
Vydáno v:	PloS one Ročník 12; číslo 6; s. e0179465
Hlavní autoři:	Ekino, Taisuke, Yoshiga, Toyoshi, Takeuchi-Kaneko, Yuko, Kanzaki, Natsumi
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	United States Public Library of Science 16.06.2017 Public Library of Science (PLoS)
Témata:	Adults Agriculture Animal Structures - metabolism Animal Structures - ultrastructure Animals Apomorphy Bark Beetles Biology and Life Sciences Body wall Bursaphelenchus Bursaphelenchus conicaudatus Bursaphelenchus luxuriosae Bursaphelenchus rainulfi Bursaphelenchus xylophilus Carriers Coleoptera - parasitology Computer and Information Sciences Cuticle Cuticles Ecology Electron microscopy Elytra Epicuticle Evolution Evolutionary biology Forest products Forestry Gene sequencing Hemocoel Insects Life history Medicine and Health Sciences Microscopy, Electron, Transmission Nematoda - genetics Nematoda - metabolism Nematoda - ultrastructure Nematodes Parasitaphelenchinae Parasites Parasitism Phylogeny Physiological aspects Proteins Ribonucleic acid RNA RNA, Helminth - genetics RNA, Helminth - metabolism RNA, Ribosomal - genetics RNA, Ribosomal - metabolism Roundworms rRNA Striations Transmission electron microscopy Japan
ISSN:	1932-6203, 1932-6203
On-line přístup:	Získat plný text
Tagy:	Přidat tag Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!

Popis
Shrnutí:	Using transmission electron microscopy, we examined the body cuticle ultrastructures of phoretic and parasitic stages of the parasitaphelenchid nematodes Bursaphelenchus xylophilus, B. conicaudatus, B. luxuriosae, B. rainulfi; an unidentified Bursaphelenchus species, and an unidentified Parasitaphelenchus species. Nematode body cuticles usually consist of three zones, a cortical zone, a median zone, and a basal zone. The phoretic stages of Bursaphelenchus spp., isolated from the tracheal systems of longhorn beetles or the elytra of bark beetles, have a thick and radially striated basal zone. In contrast, the parasitic stage of Parasitaphelenchus sp., isolated from bark beetle hemocoel, has no radial striations in the basal zone. This difference probably reflects the peculiar ecological characteristics of the phoretic stage. A well-developed basal radially striated zone, composed of very closely linked proteins, is the zone closest to the body wall muscle. Therefore, the striation is necessary for the phoretic species to be able to seek, enter, and depart from host/carrier insects, but is not essential for internal parasites in parasitaphelenchid nematodes. Phylogenetic relationships inferred from near-full-length small subunit ribosomal RNA sequences suggest that the cuticle structures of parasitic species have apomorphic characters, e.g., lack of striation in the basal zone, concurrent with the evolution of insect parasitism from a phoretic life history.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 Competing Interests: The authors have declared that no competing interests exist. Conceptualization: TE TY YT NK.Data curation: TE NK.Formal analysis: TE NK.Funding acquisition: YT NK.Investigation: TE NK.Methodology: TE TY NK.Project administration: TY NK.Resources: TY YT NK.Supervision: NK.Validation: TE NK.Visualization: TE NK.Writing – original draft: TE NK.Writing – review & editing: TE TY YT NK. Current address: Kansai Research Center, Forestry and Forest Products Research Institute, Fushimi, Kyoto, Japan
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0179465