T4-Related Bacteriophage LIMEstone Isolates for the Control of Soft Rot on Potato Caused by ‘Dickeya solani’

The bacterium 'Dickeya solani', an aggressive biovar 3 variant of Dickeya dianthicola, causes rotting and blackleg in potato. To control this pathogen using bacteriophage therapy, we isolated and characterized two closely related and specific bacteriophages, vB_DsoM_LIMEstone1 and vB_DsoM_...

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Vydáno v:	PloS one Ročník 7; číslo 3; s. e33227
Hlavní autoři:	Adriaenssens, Evelien M., Van Vaerenbergh, Johan, Vandenheuvel, Dieter, Dunon, Vincent, Ceyssens, Pieter-Jan, De Proft, Maurice, Kropinski, Andrew M., Noben, Jean-Paul, Maes, Martine, Lavigne, Rob
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	United States Public Library of Science 07.03.2012 Public Library of Science (PLoS)
Témata:	Agriculture Antibiotics Bacteria Bacteriophage T4 - isolation & purification Bacteriophage T4 - physiology Bacteriophage T4 - ultrastructure Bioinformatics Biological control Biological pest control Biology Blackleg Crop diseases Deoxyribonucleic acid DNA Enterobacteriaceae - virology Fisheries Gene Order Genetic aspects Genome, Viral Genomes Genomics Limestone Molecular Sequence Annotation Molecular Sequence Data Pathogens Pectobacterium carotovorum Phage vii Phages Plant Diseases - prevention & control Plants (botany) Potatoes Proteins Pseudomonas aeruginosa Salmonella Soft rot Solanum tuberosum Solanum tuberosum - microbiology Therapy Tubers Vegetables Web sites Websites Belgium
ISSN:	1932-6203, 1932-6203
On-line přístup:	Získat plný text
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Popis
Shrnutí:	The bacterium 'Dickeya solani', an aggressive biovar 3 variant of Dickeya dianthicola, causes rotting and blackleg in potato. To control this pathogen using bacteriophage therapy, we isolated and characterized two closely related and specific bacteriophages, vB_DsoM_LIMEstone1 and vB_DsoM_LIMEstone2. The LIMEstone phages have a T4-related genome organization and share DNA similarity with Salmonella phage ViI. Microbiological and molecular characterization of the phages deemed them suitable and promising for use in phage therapy. The phages reduced disease incidence and severity on potato tubers in laboratory assays. In addition, in a field trial of potato tubers, when infected with 'Dickeya solani', the experimental phage treatment resulted in a higher yield. These results form the basis for the development of a bacteriophage-based biocontrol of potato plants and tubers as an alternative for the use of antibiotics.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 ObjectType-Article-2 ObjectType-Feature-1 content type line 23 Conceived and designed the experiments: EMA JVV PJC MDP MM RL. Performed the experiments: EMA DV VD AMK JPN. Analyzed the data: EMA DV VD PJC AMK JPN MDP MM RL. Contributed reagents/materials/analysis tools: AMK JPN MDP MM RL. Wrote the paper: EMA JVV DV VD PJC MDP AMK JPN MM RL.
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0033227