T4-Related Bacteriophage LIMEstone Isolates for the Control of Soft Rot on Potato Caused by ‘Dickeya solani’

The bacterium 'Dickeya solani', an aggressive biovar 3 variant of Dickeya dianthicola, causes rotting and blackleg in potato. To control this pathogen using bacteriophage therapy, we isolated and characterized two closely related and specific bacteriophages, vB_DsoM_LIMEstone1 and vB_DsoM_...

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Published in:PloS one Vol. 7; no. 3; p. e33227
Main Authors: Adriaenssens, Evelien M., Van Vaerenbergh, Johan, Vandenheuvel, Dieter, Dunon, Vincent, Ceyssens, Pieter-Jan, De Proft, Maurice, Kropinski, Andrew M., Noben, Jean-Paul, Maes, Martine, Lavigne, Rob
Format: Journal Article
Language:English
Published: United States Public Library of Science 07.03.2012
Public Library of Science (PLoS)
Subjects:
Agriculture
Antibiotics
Bacteria
Bacteriophage T4 - isolation & purification
Bacteriophage T4 - physiology
Bacteriophage T4 - ultrastructure
Bioinformatics
Biological control
Biological pest control
Biology
Blackleg
Crop diseases
Deoxyribonucleic acid
DNA
Enterobacteriaceae - virology
Fisheries
Gene Order
Genetic aspects
Genome, Viral
Genomes
Genomics
Limestone
Molecular Sequence Annotation
Molecular Sequence Data
Pathogens
Pectobacterium carotovorum
Phage vii
Phages
Plant Diseases - prevention & control
Plants (botany)
Potatoes
Proteins
Pseudomonas aeruginosa
Salmonella
Soft rot
Solanum tuberosum
Solanum tuberosum - microbiology
Therapy
Tubers
Vegetables
Web sites
Websites
Belgium
ISSN:1932-6203, 1932-6203
Online Access:Get full text
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Summary:The bacterium 'Dickeya solani', an aggressive biovar 3 variant of Dickeya dianthicola, causes rotting and blackleg in potato. To control this pathogen using bacteriophage therapy, we isolated and characterized two closely related and specific bacteriophages, vB_DsoM_LIMEstone1 and vB_DsoM_LIMEstone2. The LIMEstone phages have a T4-related genome organization and share DNA similarity with Salmonella phage ViI. Microbiological and molecular characterization of the phages deemed them suitable and promising for use in phage therapy. The phages reduced disease incidence and severity on potato tubers in laboratory assays. In addition, in a field trial of potato tubers, when infected with 'Dickeya solani', the experimental phage treatment resulted in a higher yield. These results form the basis for the development of a bacteriophage-based biocontrol of potato plants and tubers as an alternative for the use of antibiotics.
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Conceived and designed the experiments: EMA JVV PJC MDP MM RL. Performed the experiments: EMA DV VD AMK JPN. Analyzed the data: EMA DV VD PJC AMK JPN MDP MM RL. Contributed reagents/materials/analysis tools: AMK JPN MDP MM RL. Wrote the paper: EMA JVV DV VD PJC MDP AMK JPN MM RL.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0033227