Lactic acid production from lime-treated wheat straw by Bacillus coagulans: neutralization of acid by fed-batch addition of alkaline substrate

Conventional processes for lignocellulose-to-organic acid conversion requires pretreatment, enzymatic hydrolysis, and microbial fermentation. In this study, lime-treated wheat straw was hydrolyzed and fermented simultaneously to lactic acid by an enzyme preparation and Bacillus coagulans DSM 2314. D...

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Bibliographic Details
Published in:	Applied microbiology and biotechnology Vol. 78; no. 5; pp. 751 - 758
Main Authors:	Maas, Ronald H. W, Bakker, Robert R, Jansen, Mickel L. A, Visser, Diana, de Jong, Ed, Eggink, Gerrit, Weusthuis, Ruud A
Format:	Journal Article
Language:	English
Published:	Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01.04.2008 Springer Berlin Heidelberg Springer Springer Nature B.V
Subjects:	Acid production Acids alkali treatment analysis Bacillus Bacillus - enzymology Bacillus - metabolism Bacillus coagulans Batch culture Biodegradation, Environmental Biological and medical sciences Biomass Bioreactors Biotechnological Products and Process Engineering Biotechnology Calcium Compounds Calcium Compounds - chemistry Calcium hydroxide Calcium Hydroxide - chemistry Cellulose Cellulose acetate chemistry Drinking water enzymatic hydrolysis Enzymes enzymology Ethanol Fed batch Fermentation Fundamental and applied biological sciences. Psychology Glucan glucans Glucose growth Hydrochloric acid Hydrogen-Ion Concentration Hydrolysis Industrial Microbiology Lactic acid Lactic Acid - chemistry Lactic Acid - metabolism Life Sciences Lignin Lignocellulose Lime Lime treatment Lime-treated wheat straw metabolism Methods. Procedures. Technologies Microbial engineering. Fermentation and microbial culture technology Microbial Genetics and Genomics Microbiology Microorganisms Neutralization Organic acids Oxides Oxides - chemistry pH control Pretreatment R&D Raw materials recycling Research & development Simultaneous saccharification and fermentation Slaked lime Straw Studies Triticum Triticum - chemistry Triticum - metabolism Triticum aestivum Waste Products Waste Products - analysis Wheat Wheat straw Xylan pH control Lime-treated wheat straw Lactic acid Calcium hydroxide Simultaneous saccharification and fermentation Lime-treated wheat straw, Simultaneous saccharification and fermentation Saccharification Lignocellulosics Bacillaceae Fermentation Bacillus coagulans Straw Lime Bacillales Production pH Bacteria Cereal Lactic acid, Calcium hydroxide, pH control Wheat Semicontinuous Neutralization
ISSN:	0175-7598, 1432-0614
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Summary:	Conventional processes for lignocellulose-to-organic acid conversion requires pretreatment, enzymatic hydrolysis, and microbial fermentation. In this study, lime-treated wheat straw was hydrolyzed and fermented simultaneously to lactic acid by an enzyme preparation and Bacillus coagulans DSM 2314. Decrease in pH because of lactic acid formation was partially adjusted by automatic addition of the alkaline substrate. After 55 h of incubation, the polymeric glucan, xylan, and arabinan present in the lime-treated straw were hydrolyzed for 55%, 75%, and 80%, respectively. Lactic acid (40.7 g/l) indicated a fermentation efficiency of 81% and a chiral l(+)-lactic acid purity of 97.2%. In total, 711 g lactic acid was produced out of 2,706 g lime-treated straw, representing 43% of the overall theoretical maximum yield. Approximately half of the lactic acid produced was neutralized by fed-batch feeding of lime-treated straw, whereas the remaining half was neutralized during the batch phase with a Ca(OH)₂ suspension. Of the lime added during the pretreatment of straw, 61% was used for the neutralization of lactic acid. This is the first demonstration of a process having a combined alkaline pretreatment of lignocellulosic biomass and pH control in fermentation resulting in a significant saving of lime consumption and avoiding the necessity to recycle lime.
Bibliography:	http://dx.doi.org/10.1007/s00253-008-1361-1 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23
ISSN:	0175-7598 1432-0614
DOI:	10.1007/s00253-008-1361-1