Cell-Specific Detection of miR-375 Downregulation for Predicting the Prognosis of Esophageal Squamous Cell Carcinoma by miRNA In Situ Hybridization

MicroRNAs (miRNAs) play important roles in the regulation of genes associated with cancer development and progression. By the more deeply characterization of miRNAs' effect in cancer development, it requires a useful tool to investigate expression and distribution of a miRNA in cancer cells and...

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Vydané v:PloS one Ročník 8; číslo 1; s. e53582
Hlavní autori: Li, Jiangchao, Li, Xiaodong, Li, Yan, Yang, Hong, Wang, Lijing, Qin, Yanru, Liu, Haibo, Fu, Li, Guan, Xin-Yuan
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: United States Public Library of Science 03.01.2013
Public Library of Science (PLoS)
Predmet:
Acids
Apoptosis
Biology
Biomarkers
Biomarkers, Tumor - metabolism
Cancer
Carcinoma, Squamous Cell - genetics
Cases (containers)
Cell growth
Cell Line, Tumor
Down-Regulation
Enzymes
Esophageal cancer
Esophageal Neoplasms - genetics
Esophageal Squamous Cell Carcinoma
Esophagus
Fluorescence
Gene expression
Gene Expression Profiling
Gene Expression Regulation, Neoplastic
Gene regulation
Genetic aspects
Human subjects
Humans
Hybridization
In situ hybridization
In Situ Hybridization - methods
Laboratories
Lung cancer
Medical prognosis
Medicine
Metastases
Metastasis
MicroRNA
MicroRNAs
MicroRNAs - metabolism
Microscopy, Fluorescence
miRNA
Neoplasm Metastasis
Oligonucleotide Array Sequence Analysis
Oncology
Phosphatase
Polymerase Chain Reaction
Prognosis
Solid tumors
Squamous cell carcinoma
Thoracic surgery
Tissue Array Analysis - methods
Tissues
Treatment Outcome
Tumor cell lines
Tumors
Hong Kong China
China
ISSN:1932-6203, 1932-6203
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Shrnutí:MicroRNAs (miRNAs) play important roles in the regulation of genes associated with cancer development and progression. By the more deeply characterization of miRNAs' effect in cancer development, it requires a useful tool to investigate expression and distribution of a miRNA in cancer cells and tissues. To fulfill this application demand, we developed a miRNA in situ hybridization (MISH) approach using the 2'-Fluoro modified miRNA probe in combination with enzyme-labeled fluorescence (ELF) signal amplification approach. MISH was used to study expression of miR-375 in esophageal squamous cell carcinoma (ESCC) cell lines and tissues using a tissue microarray (TMA) containing 300 cases. The results showed that our MISH approach is a practical way to detect expression and distribution of a tested miRNA in both cultured cells and archive tissue sections. MISH results also showed that miR-375 was frequently downregulated in ESCCs, which was significantly associated with advanced clinical stage (p = 0.003) tumor metastasis (p = 0.04) and poor outcome (p = 0.04) of ESCC. Moreover, the accuracy of MISH results could be confirmed by QRT-PCR. Our results demonstrated that MISH is a useful and reliable tool to study miRNA expression in solid tumors. Downregulation of miR-375 can be used as a biomarker to predict the outcome of ESCC.
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SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Conceived and designed the experiments: JL XYG. Performed the experiments: JL XL. Analyzed the data: JL XL. Contributed reagents/materials/analysis tools: YL HY LW YRQ HL LF. Wrote the paper: JL XYG.
Competing Interests: The authors have declared that no competing interests exist.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0053582