Impact of long-term storage and freeze-thawing on eight circulating microRNAs in plasma samples

Sample collection, processing, storage and isolation methods constitute pre-analytic factors that can influence the quality of samples used in research and clinical practice. With regard to biobanking practices, a critical point in the sample's life chain is storage, particularly long-term stor...

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Vydáno v:	PloS one Ročník 15; číslo 1; s. e0227648
Hlavní autoři:	Matias-Garcia, Pamela R., Wilson, Rory, Mussack, Veronika, Reischl, Eva, Waldenberger, Melanie, Gieger, Christian, Anton, Gabriele, Peters, Annette, Kuehn-Steven, Andrea
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	United States Public Library of Science 14.01.2020 Public Library of Science (PLoS)
Témata:	Adult Agent Orange Biological markers Biological Specimen Banks - standards Biology and life sciences Biomarkers Biomarkers - blood Chronic diseases Chronic illnesses Circulating MicroRNA - blood Circulating MicroRNA - genetics Circulating MicroRNA - isolation & purification Cohort Studies Collection Computer and Information Sciences Contamination Criminal investigation Critical point Data visualization EDTA Environmental impact Female Freeze thaw cycles Freeze-thawing Freezing - adverse effects Gene Expression Profiling - methods Humans Liquid nitrogen Low temperature Medicine and Health Sciences MicroRNA MicroRNAs MicroRNAs - blood MicroRNAs - genetics MicroRNAs - isolation & purification miRNA Physical Sciences Polls & surveys Population studies Profiling Research and Analysis Methods RNA Stability Room temperature Specimen Handling - methods Statistical analysis Statistical methods Storage Studies Surveys Thawing Time Transcriptome - genetics Vapor phases Variance analysis Germany
ISSN:	1932-6203, 1932-6203
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