Impact of long-term storage and freeze-thawing on eight circulating microRNAs in plasma samples

Sample collection, processing, storage and isolation methods constitute pre-analytic factors that can influence the quality of samples used in research and clinical practice. With regard to biobanking practices, a critical point in the sample's life chain is storage, particularly long-term stor...

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Vydáno v:	PloS one Ročník 15; číslo 1; s. e0227648
Hlavní autoři:	Matias-Garcia, Pamela R., Wilson, Rory, Mussack, Veronika, Reischl, Eva, Waldenberger, Melanie, Gieger, Christian, Anton, Gabriele, Peters, Annette, Kuehn-Steven, Andrea
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	United States Public Library of Science 14.01.2020 Public Library of Science (PLoS)
Témata:	Adult Agent Orange Biological markers Biological Specimen Banks - standards Biology and life sciences Biomarkers Biomarkers - blood Chronic diseases Chronic illnesses Circulating MicroRNA - blood Circulating MicroRNA - genetics Circulating MicroRNA - isolation & purification Cohort Studies Collection Computer and Information Sciences Contamination Criminal investigation Critical point Data visualization EDTA Environmental impact Female Freeze thaw cycles Freeze-thawing Freezing - adverse effects Gene Expression Profiling - methods Humans Liquid nitrogen Low temperature Medicine and Health Sciences MicroRNA MicroRNAs MicroRNAs - blood MicroRNAs - genetics MicroRNAs - isolation & purification miRNA Physical Sciences Polls & surveys Population studies Profiling Research and Analysis Methods RNA Stability Room temperature Specimen Handling - methods Statistical analysis Statistical methods Storage Studies Surveys Thawing Time Transcriptome - genetics Vapor phases Variance analysis Germany
ISSN:	1932-6203, 1932-6203
On-line přístup:	Získat plný text
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Popis
Shrnutí:	Sample collection, processing, storage and isolation methods constitute pre-analytic factors that can influence the quality of samples used in research and clinical practice. With regard to biobanking practices, a critical point in the sample's life chain is storage, particularly long-term storage. Since most studies examine the influence of different temperatures (4°C, room temperature) or delays in sample processing on sample quality, there is only little information on the effects of long-term storage at ultra-low (vapor phase of liquid nitrogen) temperatures on biomarker levels. Among these biomarkers, circulating miRNAs hold great potential for diagnosis or prognosis for a variety of diseases, like cancer, infections and chronic diseases, and are thus of high interest in several scientific questions. We therefore investigated the influence of long-term storage on levels of eight circulating miRNAs (miR-103a-3p, miR-191-5p, miR-124-3p, miR-30c-5p, miR-451a, miR-23a-3p, miR-93-5p, miR-24-3p, and miR-33b-5p) from 10 participants from the population-based cohort study KORA. Sample collection took place during the baseline survey S4 and the follow-up surveys F4 and FF4, over a time period spanning from 1999 to 2014. The influence of freeze-thaw (f/t) cycles on miRNA stability was also investigated using samples from volunteers (n = 6). Obtained plasma samples were profiled using Exiqon's miRCURYTM real-time PCR profiling system, and repeated measures ANOVA was used to check for storage or f/t effects. Our results show that detected levels of most of the studied miRNAs showed no statistically significant changes due to storage at ultra-low temperatures for up to 17 years; miR-451a levels were altered due to contamination during sampling. Freeze-thawing of one to four cycles showed an effect only on miR-30c-5p. Our results highlight the robustness of this set of circulating miRNAs for decades of storage at ultra-low temperatures and several freeze-thaw cycles, which makes our findings increasingly relevant for research conducted with biobanked samples.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 KORA-Study Group, Helmholtz Zentrum Muenchen, German Research Center for Environmental Health, Neuherberg, Germany. Membership of the KORA study group is provided in the Acknowledgments. Competing Interests: The authors have declared that no competing interests exist.
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0227648