Generation of Induced Pluripotent Stem Cells from Human Nasal Epithelial Cells Using a Sendai Virus Vector

The generation of induced pluripotent stem cells (iPSCs) by introducing reprogramming factors into somatic cells is a promising method for stem cell therapy in regenerative medicine. Therefore, it is desirable to develop a minimally invasive simple method to create iPSCs. In this study, we generated...

Celý popis

Uložené v:

Podrobná bibliografia
Vydané v:	PloS one Ročník 7; číslo 8; s. e42855
Hlavní autori:	Ono, Mizuho, Hamada, Yuko, Horiuchi, Yasue, Matsuo-Takasaki, Mami, Imoto, Yoshimasa, Satomi, Kaishi, Arinami, Tadao, Hasegawa, Mamoru, Fujioka, Tsuyoshi, Nakamura, Yukio, Noguchi, Emiko
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	United States Public Library of Science 13.08.2012 Public Library of Science (PLoS)
Predmet:	Anesthesia B cells Biology Biopsy Blood Cellular Reprogramming DNA methylation Efficiency Embryo cells Embryonic stem cells Epigenetic inheritance Epigenetics Epithelial cells Female Fibroblasts Gene expression Genes Genetic vectors Genetic Vectors - genetics Genomes Genomics Genotypes Health aspects Humans Induced Pluripotent Stem Cells - cytology Induced Pluripotent Stem Cells - metabolism Infections Lymphocytes Medical research Medicine Molecular modelling Morphology Nasal Mucosa - cytology Otolaryngology Pathogenesis Pluripotency Proteins Regenerative medicine Sendai virus - genetics Somatic cells Stem cell transplantation Stem cells Transduction, Genetic - methods Vectors Viruses Japan
ISSN:	1932-6203, 1932-6203
On-line prístup:	Získať plný text
Tagy:	Pridať tag Žiadne tagy, Buďte prvý, kto otaguje tento záznam!

Abstract	The generation of induced pluripotent stem cells (iPSCs) by introducing reprogramming factors into somatic cells is a promising method for stem cell therapy in regenerative medicine. Therefore, it is desirable to develop a minimally invasive simple method to create iPSCs. In this study, we generated human nasal epithelial cells (HNECs)-derived iPSCs by gene transduction with Sendai virus (SeV) vectors. HNECs can be obtained from subjects in a noninvasive manner, without anesthesia or biopsy. In addition, SeV carries no risk of altering the host genome, which provides an additional level of safety during generation of human iPSCs. The multiplicity of SeV infection ranged from 3 to 4, and the reprogramming efficiency of HNECs was 0.08-0.10%. iPSCs derived from HNECs had global gene expression profiles and epigenetic states consistent with those of human embryonic stem cells. The ease with which HNECs can be obtained, together with their robust reprogramming characteristics, will provide opportunities to investigate disease pathogenesis and molecular mechanisms in vitro, using cells with particular genotypes.
AbstractList	The generation of induced pluripotent stem cells (iPSCs) by introducing reprogramming factors into somatic cells is a promising method for stem cell therapy in regenerative medicine. Therefore, it is desirable to develop a minimally invasive simple method to create iPSCs. In this study, we generated human nasal epithelial cells (HNECs)-derived iPSCs by gene transduction with Sendai virus (SeV) vectors. HNECs can be obtained from subjects in a noninvasive manner, without anesthesia or biopsy. In addition, SeV carries no risk of altering the host genome, which provides an additional level of safety during generation of human iPSCs. The multiplicity of SeV infection ranged from 3 to 4, and the reprogramming efficiency of HNECs was 0.08-0.10%. iPSCs derived from HNECs had global gene expression profiles and epigenetic states consistent with those of human embryonic stem cells. The ease with which HNECs can be obtained, together with their robust reprogramming characteristics, will provide opportunities to investigate disease pathogenesis and molecular mechanisms in vitro, using cells with particular genotypes.The generation of induced pluripotent stem cells (iPSCs) by introducing reprogramming factors into somatic cells is a promising method for stem cell therapy in regenerative medicine. Therefore, it is desirable to develop a minimally invasive simple method to create iPSCs. In this study, we generated human nasal epithelial cells (HNECs)-derived iPSCs by gene transduction with Sendai virus (SeV) vectors. HNECs can be obtained from subjects in a noninvasive manner, without anesthesia or biopsy. In addition, SeV carries no risk of altering the host genome, which provides an additional level of safety during generation of human iPSCs. The multiplicity of SeV infection ranged from 3 to 4, and the reprogramming efficiency of HNECs was 0.08-0.10%. iPSCs derived from HNECs had global gene expression profiles and epigenetic states consistent with those of human embryonic stem cells. The ease with which HNECs can be obtained, together with their robust reprogramming characteristics, will provide opportunities to investigate disease pathogenesis and molecular mechanisms in vitro, using cells with particular genotypes. The generation of induced pluripotent stem cells (iPSCs) by introducing reprogramming factors into somatic cells is a promising method for stem cell therapy in regenerative medicine. Therefore, it is desirable to develop a minimally invasive simple method to create iPSCs. In this study, we generated human nasal epithelial cells (HNECs)-derived iPSCs by gene transduction with Sendai virus (SeV) vectors. HNECs can be obtained from subjects in a noninvasive manner, without anesthesia or biopsy. In addition, SeV carries no risk of altering the host genome, which provides an additional level of safety during generation of human iPSCs. The multiplicity of SeV infection ranged from 3 to 4, and the reprogramming efficiency of HNECs was 0.08–0.10%. iPSCs derived from HNECs had global gene expression profiles and epigenetic states consistent with those of human embryonic stem cells. The ease with which HNECs can be obtained, together with their robust reprogramming characteristics, will provide opportunities to investigate disease pathogenesis and molecular mechanisms in vitro, using cells with particular genotypes.
Audience	Academic
Author	Satomi, Kaishi Noguchi, Emiko Fujioka, Tsuyoshi Horiuchi, Yasue Hasegawa, Mamoru Hamada, Yuko Imoto, Yoshimasa Nakamura, Yukio Ono, Mizuho Arinami, Tadao Matsuo-Takasaki, Mami
AuthorAffiliation	1 Department of Medical Genetics, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan 6 DNAVEC Research Inc, Tsukuba, Ibaraki, Japan 2 Department of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America 7 Cell Engineering Division, RIKEN BioResource Center, Tsukuba, Ibaraki, Japan 3 Department of Regenerative Medicine and Stem Cell Biology, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan 8 Japan Science and Technology Agency, Core Research for Evolutional Science and Technology (CREST), Chiyoda, Tokyo, Japan University of Milan, Italy 4 Department of Otorhinolaryngology Head and Neck Surgery, Faculty of Medical Sciences, University of Fukui, Fukui City, Fukui, Japan 5 Department of Diagnostic Pathology, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan
AuthorAffiliation_xml	– name: 6 DNAVEC Research Inc, Tsukuba, Ibaraki, Japan – name: 3 Department of Regenerative Medicine and Stem Cell Biology, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan – name: 8 Japan Science and Technology Agency, Core Research for Evolutional Science and Technology (CREST), Chiyoda, Tokyo, Japan – name: 1 Department of Medical Genetics, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan – name: 2 Department of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America – name: University of Milan, Italy – name: 4 Department of Otorhinolaryngology Head and Neck Surgery, Faculty of Medical Sciences, University of Fukui, Fukui City, Fukui, Japan – name: 7 Cell Engineering Division, RIKEN BioResource Center, Tsukuba, Ibaraki, Japan – name: 5 Department of Diagnostic Pathology, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan
Author_xml	– sequence: 1 givenname: Mizuho surname: Ono fullname: Ono, Mizuho – sequence: 2 givenname: Yuko surname: Hamada fullname: Hamada, Yuko – sequence: 3 givenname: Yasue surname: Horiuchi fullname: Horiuchi, Yasue – sequence: 4 givenname: Mami surname: Matsuo-Takasaki fullname: Matsuo-Takasaki, Mami – sequence: 5 givenname: Yoshimasa surname: Imoto fullname: Imoto, Yoshimasa – sequence: 6 givenname: Kaishi surname: Satomi fullname: Satomi, Kaishi – sequence: 7 givenname: Tadao surname: Arinami fullname: Arinami, Tadao – sequence: 8 givenname: Mamoru surname: Hasegawa fullname: Hasegawa, Mamoru – sequence: 9 givenname: Tsuyoshi surname: Fujioka fullname: Fujioka, Tsuyoshi – sequence: 10 givenname: Yukio surname: Nakamura fullname: Nakamura, Yukio – sequence: 11 givenname: Emiko surname: Noguchi fullname: Noguchi, Emiko
BackLink	https://www.ncbi.nlm.nih.gov/pubmed/22912751$$D View this record in MEDLINE/PubMed
BookMark	eNqNk9tqGzEQhpeS0hzaNyitoFDaC7urw556UQghTQyhKXXjWyFrR7aMVnIkbWnfvnK8CXYIpezFitE3_4x-Zo6zA-ssZNlrnI8xrfCnleu9FWa8TuFxnjNSF8Wz7Ag3lIxKktODnfNhdhzCKs8LWpfli-yQkAaTqsBH2eoCLHgRtbPIKTSxbS-hRd9N7_XaRbARTSN06AyMCUh516HLvhMWfRNBGHS-1nEJRqfjlrgJ2i6QQFOwrdBopn0f0AxkdP5l9lwJE-DV8D_Jbr6e_zy7HF1dX0zOTq9GsirqOGqhEZI0DS5FLmvRgKRK1bSRGDNcFpWoVAmtnJdMlUIoXBFVFWUt54JiAqyhJ9nbre7auMAHmwLHlJSEEUzrREy2ROvEiq-97oT_w53Q_C7g_IILH7U0wKGiogYqixoaxhSZk3nVKiCpoaZiclPty1Ctn3epr-SYF2ZPdP_G6iVfuF-cMlyTGieBD4OAd7c9hMg7HWQyU1hwfeo7p6xqyrxkCX33CH36dQO1EOkB2iqX6sqNKD8tGKV5lWxM1PgJKn0tdFqmmVI6xfcSPu4lJCbC77gQfQh8Mv3x_-z1bJ99v8MuQZi4DM70m5EM--CbXacfLL4f5gSwLSC9C8GDekBwzjc7c28X3-wMH3YmpX1-lCZ1vNuI5Ig2_07-C095HEo
CitedBy_id	crossref_primary_10_1016_j_omtm_2023_07_005 crossref_primary_10_1016_j_scr_2021_102351 crossref_primary_10_1016_j_jaci_2014_08_038 crossref_primary_10_1038_srep03474 crossref_primary_10_1177_1010428320962588 crossref_primary_10_1242_dmm_041368 crossref_primary_10_1371_journal_pone_0186879 crossref_primary_10_1186_s12864_017_4389_8 crossref_primary_10_3389_fcell_2025_1593207 crossref_primary_10_1371_journal_pone_0059908 crossref_primary_10_1089_scd_2025_0082 crossref_primary_10_7717_peerj_4370 crossref_primary_10_1016_j_neulet_2019_03_029 crossref_primary_10_5966_sctm_2015_0409 crossref_primary_10_1016_j_redox_2025_103773 crossref_primary_10_1007_s00795_020_00264_4 crossref_primary_10_1002_stem_3326 crossref_primary_10_1016_j_envpol_2024_123396 crossref_primary_10_1002_term_2021 crossref_primary_10_1016_j_gene_2018_11_069 crossref_primary_10_1016_j_rpor_2018_04_005 crossref_primary_10_1038_mtm_2015_23 crossref_primary_10_1371_journal_pone_0103485 crossref_primary_10_1089_ars_2019_7983 crossref_primary_10_1007_s12015_022_10402_3 crossref_primary_10_1371_journal_pone_0064496 crossref_primary_10_1016_j_bbrc_2024_150124 crossref_primary_10_3390_cells10123483 crossref_primary_10_1002_advs_202500221 crossref_primary_10_1038_s41598_023_39065_x crossref_primary_10_1186_s13045_014_0050_z crossref_primary_10_1016_j_neures_2013_09_010 crossref_primary_10_1111_cpr_12162
ContentType	Journal Article
Copyright	COPYRIGHT 2012 Public Library of Science Ono et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. 2012 Ono et al 2012 Ono et al
Copyright_xml	– notice: COPYRIGHT 2012 Public Library of Science – notice: Ono et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. – notice: 2012 Ono et al 2012 Ono et al
DBID	AAYXX CITATION CGR CUY CVF ECM EIF NPM IOV ISR 3V. 7QG 7QL 7QO 7RV 7SN 7SS 7T5 7TG 7TM 7U9 7X2 7X7 7XB 88E 8AO 8C1 8FD 8FE 8FG 8FH 8FI 8FJ 8FK ABJCF ABUWG AEUYN AFKRA ARAPS ATCPS AZQEC BBNVY BENPR BGLVJ BHPHI C1K CCPQU D1I DWQXO FR3 FYUFA GHDGH GNUQQ H94 HCIFZ K9. KB. KB0 KL. L6V LK8 M0K M0S M1P M7N M7P M7S NAPCQ P5Z P62 P64 PATMY PDBOC PHGZM PHGZT PIMPY PJZUB PKEHL PPXIY PQEST PQGLB PQQKQ PQUKI PRINS PTHSS PYCSY RC3 7X8 5PM DOA
DOI	10.1371/journal.pone.0042855
DatabaseName	CrossRef Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed Gale In Context: Opposing Viewpoints Gale In Context: Science ProQuest Central (Corporate) Animal Behavior Abstracts Bacteriology Abstracts (Microbiology B) Biotechnology Research Abstracts Nursing & Allied Health Database Ecology Abstracts Entomology Abstracts (Full archive) Immunology Abstracts Meteorological & Geoastrophysical Abstracts Nucleic Acids Abstracts Virology and AIDS Abstracts Agricultural Science Collection Health & Medical Collection ProQuest Central (purchase pre-March 2016) Medical Database (Alumni Edition) ProQuest Pharma Collection Public Health Database Technology Research Database ProQuest SciTech Collection ProQuest Technology Collection ProQuest Natural Science Collection Hospital Premium Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) Materials Science & Engineering Collection ProQuest Central (Alumni) ProQuest One Sustainability ProQuest Central UK/Ireland Advanced Technologies & Computer Science Collection Agricultural & Environmental Science Collection ProQuest Central Essentials - QC ProQuest SciTech Premium Collection Natural Science Collection Biological Science Collection ProQuest Central ProQuest Technology Collection Natural Science Collection Environmental Sciences and Pollution Management ProQuest One ProQuest Materials Science Collection ProQuest Central Korea Engineering Research Database Proquest Health Research Premium Collection Health Research Premium Collection (Alumni) ProQuest Central Student AIDS and Cancer Research Abstracts SciTech Premium Collection ProQuest Health & Medical Complete (Alumni) Materials Science Database Nursing & Allied Health Database (Alumni Edition) Meteorological & Geoastrophysical Abstracts - Academic ProQuest Engineering Collection ProQuest Biological Science Collection Agricultural Science Database ProQuest Health & Medical Collection Medical Database Algology Mycology and Protozoology Abstracts (Microbiology C) Biological Science Database Engineering Database Nursing & Allied Health Premium Advanced Technologies & Aerospace Database ProQuest Advanced Technologies & Aerospace Collection Biotechnology and BioEngineering Abstracts Environmental Science Database Materials Science Collection ProQuest Central Premium ProQuest One Academic (New) ProQuest Publicly Available Content Database ProQuest Health & Medical Research Collection ProQuest One Academic Middle East (New) ProQuest One Health & Nursing ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Applied & Life Sciences ProQuest One Academic (retired) ProQuest One Academic UKI Edition ProQuest Central China Engineering Collection Environmental Science Collection Genetics Abstracts MEDLINE - Academic PubMed Central (Full Participant titles) DOAJ Directory of Open Access Journals
DatabaseTitle	CrossRef MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) Agricultural Science Database Publicly Available Content Database ProQuest Central Student ProQuest Advanced Technologies & Aerospace Collection ProQuest Central Essentials Nucleic Acids Abstracts SciTech Premium Collection ProQuest Central China Environmental Sciences and Pollution Management ProQuest One Applied & Life Sciences ProQuest One Sustainability Health Research Premium Collection Meteorological & Geoastrophysical Abstracts Natural Science Collection Health & Medical Research Collection Biological Science Collection ProQuest Central (New) ProQuest Medical Library (Alumni) Engineering Collection Advanced Technologies & Aerospace Collection Engineering Database Virology and AIDS Abstracts ProQuest Biological Science Collection ProQuest One Academic Eastern Edition Agricultural Science Collection ProQuest Hospital Collection ProQuest Technology Collection Health Research Premium Collection (Alumni) Biological Science Database Ecology Abstracts ProQuest Hospital Collection (Alumni) Biotechnology and BioEngineering Abstracts Environmental Science Collection Entomology Abstracts Nursing & Allied Health Premium ProQuest Health & Medical Complete ProQuest One Academic UKI Edition Environmental Science Database ProQuest Nursing & Allied Health Source (Alumni) Engineering Research Database ProQuest One Academic Meteorological & Geoastrophysical Abstracts - Academic ProQuest One Academic (New) Technology Collection Technology Research Database ProQuest One Academic Middle East (New) Materials Science Collection ProQuest Health & Medical Complete (Alumni) ProQuest Central (Alumni Edition) ProQuest One Community College ProQuest One Health & Nursing ProQuest Natural Science Collection ProQuest Pharma Collection ProQuest Central ProQuest Health & Medical Research Collection Genetics Abstracts ProQuest Engineering Collection Biotechnology Research Abstracts Health and Medicine Complete (Alumni Edition) ProQuest Central Korea Bacteriology Abstracts (Microbiology B) Algology Mycology and Protozoology Abstracts (Microbiology C) Agricultural & Environmental Science Collection AIDS and Cancer Research Abstracts Materials Science Database ProQuest Materials Science Collection ProQuest Public Health ProQuest Nursing & Allied Health Source ProQuest SciTech Collection Advanced Technologies & Aerospace Database ProQuest Medical Library Animal Behavior Abstracts Materials Science & Engineering Collection Immunology Abstracts ProQuest Central (Alumni) MEDLINE - Academic
DatabaseTitleList	MEDLINE - Academic Agricultural Science Database MEDLINE
Database_xml	– sequence: 1 dbid: DOA name: 开放获取期刊（Open Access Journals） url: https://www.doaj.org/ sourceTypes: Open Website – sequence: 2 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 3 dbid: PIMPY name: ProQuest Publicly Available Content Database url: http://search.proquest.com/publiccontent sourceTypes: Aggregation Database
DeliveryMethod	fulltext_linktorsrc
Discipline	Sciences (General) Medicine Biology
DocumentTitleAlternate	iPS Cells Generated from Nasal Epithelial Cells
EISSN	1932-6203
ExternalDocumentID	1326242138 oai_doaj_org_article_e73a8e3c58e944f2b2b7dfe2839974c9 PMC3418281 2942747381 A543307165 22912751 10_1371_journal_pone_0042855
Genre	Research Support, Non-U.S. Gov't Journal Article
GeographicLocations	Japan
GeographicLocations_xml	– name: Japan
GroupedDBID	--- 123 29O 2WC 53G 5VS 7RV 7X2 7X7 7XC 88E 8AO 8C1 8CJ 8FE 8FG 8FH 8FI 8FJ A8Z AAFWJ AAUCC AAWOE AAYXX ABDBF ABIVO ABJCF ABUWG ACCTH ACGFO ACIHN ACIWK ACPRK ACUHS ADBBV ADRAZ AEAQA AENEX AEUYN AFFHD AFKRA AFPKN AFRAH AHMBA ALMA_UNASSIGNED_HOLDINGS AOIJS APEBS ARAPS ATCPS BAWUL BBNVY BCNDV BENPR BGLVJ BHPHI BKEYQ BPHCQ BVXVI BWKFM CCPQU CITATION CS3 D1I D1J D1K DIK DU5 E3Z EAP EAS EBD EMOBN ESX EX3 F5P FPL FYUFA GROUPED_DOAJ GX1 HCIFZ HH5 HMCUK HYE IAO IEA IGS IHR IHW INH INR IOV IPNFZ IPY ISE ISR ITC K6- KB. KQ8 L6V LK5 LK8 M0K M1P M48 M7P M7R M7S M~E NAPCQ O5R O5S OK1 OVT P2P P62 PATMY PDBOC PHGZM PHGZT PIMPY PJZUB PPXIY PQGLB PQQKQ PROAC PSQYO PTHSS PYCSY RIG RNS RPM SV3 TR2 UKHRP WOQ WOW ~02 ~KM 3V. ALIPV BBORY CGR CUY CVF ECM EIF NPM PV9 RZL 7QG 7QL 7QO 7SN 7SS 7T5 7TG 7TM 7U9 7XB 8FD 8FK AZQEC C1K DWQXO ESTFP FR3 GNUQQ H94 K9. KL. M7N P64 PKEHL PQEST PQUKI PRINS RC3 7X8 PUEGO 5PM AAPBV ABPTK N95
ID	FETCH-LOGICAL-c758t-de9ac29916a0c8a9ec3ff839c1141657a7f6edcb64f6aaf172f7568cba312e493
IEDL.DBID	FPL
ISICitedReferencesCount	41
ISICitedReferencesURI	http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000307500100038&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
ISSN	1932-6203
IngestDate	Sun May 07 16:28:22 EDT 2023 Fri Oct 03 12:51:33 EDT 2025 Tue Nov 04 01:57:23 EST 2025 Thu Oct 02 09:38:29 EDT 2025 Tue Oct 07 07:02:43 EDT 2025 Sat Nov 29 13:15:20 EST 2025 Sat Nov 29 10:01:44 EST 2025 Wed Nov 26 10:12:33 EST 2025 Wed Nov 26 10:10:23 EST 2025 Thu May 22 21:19:51 EDT 2025 Wed Feb 19 01:49:05 EST 2025 Sat Nov 29 02:35:44 EST 2025 Tue Nov 18 21:33:41 EST 2025
IsDoiOpenAccess	true
IsOpenAccess	true
IsPeerReviewed	true
IsScholarly	true
Issue	8
Language	English
License	This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. Creative Commons Attribution License
LinkModel	DirectLink
MergedId	FETCHMERGED-LOGICAL-c758t-de9ac29916a0c8a9ec3ff839c1141657a7f6edcb64f6aaf172f7568cba312e493
Notes	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 Conceived and designed the experiments: Y. Horiuchi MMT TA EN. Performed the experiments: MO Y. Hamada YI KS TF. Analyzed the data: MO TH EN. Contributed reagents/materials/analysis tools: MMT YN MH. Wrote the paper: MO Y. Hamada MMT EN. Competing Interests: The authors have declared that no competing interests exist.
OpenAccessLink	http://dx.doi.org/10.1371/journal.pone.0042855
PMID	22912751
PQID	1326242138
PQPubID	1436336
PageCount	e42855
ParticipantIDs	plos_journals_1326242138 doaj_primary_oai_doaj_org_article_e73a8e3c58e944f2b2b7dfe2839974c9 pubmedcentral_primary_oai_pubmedcentral_nih_gov_3418281 proquest_miscellaneous_1034796064 proquest_journals_1326242138 gale_infotracmisc_A543307165 gale_infotracacademiconefile_A543307165 gale_incontextgauss_ISR_A543307165 gale_incontextgauss_IOV_A543307165 gale_healthsolutions_A543307165 pubmed_primary_22912751 crossref_primary_10_1371_journal_pone_0042855 crossref_citationtrail_10_1371_journal_pone_0042855
PublicationCentury	2000
PublicationDate	2012-08-13
PublicationDateYYYYMMDD	2012-08-13
PublicationDate_xml	– month: 08 year: 2012 text: 2012-08-13 day: 13
PublicationDecade	2010
PublicationPlace	United States
PublicationPlace_xml	– name: United States – name: San Francisco – name: San Francisco, USA
PublicationTitle	PloS one
PublicationTitleAlternate	PLoS One
PublicationYear	2012
Publisher	Public Library of Science Public Library of Science (PLoS)
Publisher_xml	– name: Public Library of Science – name: Public Library of Science (PLoS)
References	21243013 - Cell Res. 2011 Mar;21(3):518-29 22132178 - PLoS One. 2011;6(11):e27956 22264161 - Stem Cells Dev. 2012 Aug 10;21(12):2322-32 18423197 - Cell. 2008 Apr 18;133(2):250-64 18045526 - Cell. 2007 Nov 30;131(5):834-5 21993621 - Nature. 2011 Oct 20;478(7369):391-4 18691744 - Cell. 2008 Sep 5;134(5):877-86 21821793 - Proc Natl Acad Sci U S A. 2011 Aug 23;108(34):14234-9 18467348 - Bioinformatics. 2008 Jul 1;24(13):1547-8 18035408 - Cell. 2007 Nov 30;131(5):861-72 20621043 - Cell Stem Cell. 2010 Jul 2;7(1):11-4 20547351 - J Biosci Bioeng. 2010 Sep;110(3):345-50 19805220 - Proc Natl Acad Sci U S A. 2009 Sep 15;106(37):15720-5 19922768 - Exp Hematol. 2010 Feb;38(2):154-62 18029452 - Science. 2007 Dec 21;318(5858):1917-20 20554890 - J Dent Res. 2010 Aug;89(8):773-8 18079707 - Nat Protoc. 2007;2(12):3081-9 17314394 - Mol Biol Cell. 2007 May;18(5):1543-53 10973218 - Nat Biotechnol. 2000 Sep;18(9):970-3 20621044 - Cell Stem Cell. 2010 Jul 2;7(1):15-9
References_xml	– reference: 20621043 - Cell Stem Cell. 2010 Jul 2;7(1):11-4 – reference: 22264161 - Stem Cells Dev. 2012 Aug 10;21(12):2322-32 – reference: 20621044 - Cell Stem Cell. 2010 Jul 2;7(1):15-9 – reference: 17314394 - Mol Biol Cell. 2007 May;18(5):1543-53 – reference: 20554890 - J Dent Res. 2010 Aug;89(8):773-8 – reference: 10973218 - Nat Biotechnol. 2000 Sep;18(9):970-3 – reference: 21821793 - Proc Natl Acad Sci U S A. 2011 Aug 23;108(34):14234-9 – reference: 18467348 - Bioinformatics. 2008 Jul 1;24(13):1547-8 – reference: 22132178 - PLoS One. 2011;6(11):e27956 – reference: 21993621 - Nature. 2011 Oct 20;478(7369):391-4 – reference: 18045526 - Cell. 2007 Nov 30;131(5):834-5 – reference: 18691744 - Cell. 2008 Sep 5;134(5):877-86 – reference: 18035408 - Cell. 2007 Nov 30;131(5):861-72 – reference: 18079707 - Nat Protoc. 2007;2(12):3081-9 – reference: 19922768 - Exp Hematol. 2010 Feb;38(2):154-62 – reference: 18029452 - Science. 2007 Dec 21;318(5858):1917-20 – reference: 20547351 - J Biosci Bioeng. 2010 Sep;110(3):345-50 – reference: 21243013 - Cell Res. 2011 Mar;21(3):518-29 – reference: 19805220 - Proc Natl Acad Sci U S A. 2009 Sep 15;106(37):15720-5 – reference: 18423197 - Cell. 2008 Apr 18;133(2):250-64
SSID	ssj0053866
Score	2.290765
Snippet	The generation of induced pluripotent stem cells (iPSCs) by introducing reprogramming factors into somatic cells is a promising method for stem cell therapy in...
SourceID	plos doaj pubmedcentral proquest gale pubmed crossref
SourceType	Open Website Open Access Repository Aggregation Database Index Database Enrichment Source
StartPage	e42855
SubjectTerms	Anesthesia B cells Biology Biopsy Blood Cellular Reprogramming DNA methylation Efficiency Embryo cells Embryonic stem cells Epigenetic inheritance Epigenetics Epithelial cells Female Fibroblasts Gene expression Genes Genetic vectors Genetic Vectors - genetics Genomes Genomics Genotypes Health aspects Humans Induced Pluripotent Stem Cells - cytology Induced Pluripotent Stem Cells - metabolism Infections Lymphocytes Medical research Medicine Molecular modelling Morphology Nasal Mucosa - cytology Otolaryngology Pathogenesis Pluripotency Proteins Regenerative medicine Sendai virus - genetics Somatic cells Stem cell transplantation Stem cells Transduction, Genetic - methods Vectors Viruses
SummonAdditionalLinks	– databaseName: DOAJ Directory of Open Access Journals dbid: DOA link: http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV3db9MwELdQxQMviPG1QAGDkICHbIkdx_HjQEwgoYEYTHuLHMceRVUSNW3_fu4cN2rQpPHAW5W7psnvzuc79z4Iea1qiBESXsVGpgwClDqJlRMi1srWTGhRJD6b8OKLPDsrLi_Vt71RX5gTNrQHHoA7tpLrwnIjCquyzLGKVbJ2FnZFBa6w8aV7iVS7YGqwwbCK8zwUynGZHge5HHVtY498mIClfXsbke_XP1rlWbds--tczr8zJ_e2otN75G7wIenJ8OwH5JZt7pODsEp7-ja0kn73gPwePiL4tHUU4m-QZE275QZsRQvu8ppiI2eKx_c9xVIT6of20Ub38Au2w4qNJaho4MAs-Suq6bmFSH5Bt4vVpqdbf_D_kPw8_fjjw6c4TFcAsYhiHddWacPQPdSJKUA2hjsHqBqIkNJcSC1dDi9a5ZnLtXbg6Dgp8sJUmqfMZoo_IrMG8Dwk1GZaGSDlEkRSVbUGLyeRUtWiFuDSFBHhO6hLE1qP4wSMZen_T5MQggzIlSigMggoIvH4rW5ovXED_3uU4siLjbP9BVCnMqhTeZM6ReQF6kA5VKGOy788ERkHcwjAROSV58DmGQ1m51zpTd-Xn79e_APT-fcJ05vA5FqAw-hQEQHvhE25JpzzCSeYADMhH6LG7lDpASOGdT8pB-jnOy2-nvxyJONNMeOuse0GeBKsMYbwNovI40HpR2QZUzgXII2InCyHCfRTSrP45XuXg9MEMX765H_I6im5A-4rwxP-lM_JbL3a2GfkttmuF_3quTcIfwD97WcQ priority: 102 providerName: Directory of Open Access Journals – databaseName: Nursing & Allied Health Database dbid: 7RV link: http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV3db9MwELegIMQLsPGxwACDkGAP2fLlOH5CY9oEEpRphWpvkePYo1OWlKbl7-fOccOCJkDiLaovTXJ3vvwu90XIK1GCjxDEha94GIGDUga-MIz5UugyYpJlgc0mnH7k43F2eiqO3Qe31qVVrm2iNdRlo_Ab-R54TVjKEMbZ2_l3H6dGYXTVjdC4Tm6EiI1Bn_nJdG2JYS-nqSuXi3m456SzO29qvWudBSzwu_Q6sl37e9s8mldNexXw_D1_8tIL6eju_z7KPXLHQVG63-nOBrmm601y65MLtm-SDbfvW_rGNafeuU_Ou0MUJ20MxckfSpf0uFqB9WkAgC_pZKkv6IGuqpZi8Qq1cQI6li1c7HCONSAVKL2jsCkLVNKJrks5o9PZYtXSqQ0lPCBfjw6_HLz33bwGEDTLln6phVQRAk4ZqAykrWJjAIAp8LnClHHJTQpMK9LEpFIagE6GszRThYzDSCcifkhGNchmi1CdSKFgKeXg7xRFKQE3BZyLkpUMQFLmkXgttly5ZuY4U6PKbYSOg1PTMTFHYedO2B7x-7PmXTOPv9C_Q43oabEVt_2hWZzlbmfnmscy07FimRZJYqIiKnhpNMA2AfeuhEeeoz7lXV1rb1DyfZbEYGCBMR55aSmwHUeN-T5nctW2-YfP038gmpwMiF47ItMAO5R0NRbwTNjma0C5PaAEo6IGy1uo_WuutPkvnYUz11p99fKLfhn_FHP4at2sgCbAqmVwmBOPPOo2UM_ZKBI4aSD0CB9srQHrhyv17Jvthg4wLIuy8PGfb-sJuQ1QN8JoQBhvk9FysdJPyU31YzlrF8-s2fgJgqB2qA priority: 102 providerName: ProQuest
Title	Generation of Induced Pluripotent Stem Cells from Human Nasal Epithelial Cells Using a Sendai Virus Vector
URI	https://www.ncbi.nlm.nih.gov/pubmed/22912751 https://www.proquest.com/docview/1326242138 https://www.proquest.com/docview/1034796064 https://pubmed.ncbi.nlm.nih.gov/PMC3418281 https://doaj.org/article/e73a8e3c58e944f2b2b7dfe2839974c9 http://dx.doi.org/10.1371/journal.pone.0042855
Volume	7
WOSCitedRecordID	wos000307500100038&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
hasFullText	1
inHoldings	1
isFullTextHit
isPrint
journalDatabaseRights	– providerCode: PRVAON databaseName: 开放获取期刊（Open Access Journals） customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: DOA dateStart: 20060101 isFulltext: true titleUrlDefault: https://www.doaj.org/ providerName: Directory of Open Access Journals – providerCode: PRVHPJ databaseName: ROAD: Directory of Open Access Scholarly Resources (ISSN International Center) customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: M~E dateStart: 20060101 isFulltext: true titleUrlDefault: https://road.issn.org providerName: ISSN International Centre – providerCode: PRVPQU databaseName: Advanced Technologies & Aerospace Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: P5Z dateStart: 20061201 isFulltext: true titleUrlDefault: https://search.proquest.com/hightechjournals providerName: ProQuest – providerCode: PRVPQU databaseName: Agricultural Science Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: M0K dateStart: 20061201 isFulltext: true titleUrlDefault: https://search.proquest.com/agriculturejournals providerName: ProQuest – providerCode: PRVPQU databaseName: Biological Science Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: M7P dateStart: 20061201 isFulltext: true titleUrlDefault: http://search.proquest.com/biologicalscijournals providerName: ProQuest – providerCode: PRVPQU databaseName: Engineering Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: M7S dateStart: 20061201 isFulltext: true titleUrlDefault: http://search.proquest.com providerName: ProQuest – providerCode: PRVPQU databaseName: Environmental Science Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: PATMY dateStart: 20061201 isFulltext: true titleUrlDefault: http://search.proquest.com/environmentalscience providerName: ProQuest – providerCode: PRVPQU databaseName: Health & Medical Collection customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: 7X7 dateStart: 20061201 isFulltext: true titleUrlDefault: https://search.proquest.com/healthcomplete providerName: ProQuest – providerCode: PRVPQU databaseName: Materials Science Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: KB. dateStart: 20061201 isFulltext: true titleUrlDefault: http://search.proquest.com/materialsscijournals providerName: ProQuest – providerCode: PRVPQU databaseName: Nursing & Allied Health Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: 7RV dateStart: 20061201 isFulltext: true titleUrlDefault: https://search.proquest.com/nahs providerName: ProQuest – providerCode: PRVPQU databaseName: ProQuest Central customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: BENPR dateStart: 20061201 isFulltext: true titleUrlDefault: https://www.proquest.com/central providerName: ProQuest – providerCode: PRVPQU databaseName: ProQuest Publicly Available Content Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: PIMPY dateStart: 20061201 isFulltext: true titleUrlDefault: http://search.proquest.com/publiccontent providerName: ProQuest – providerCode: PRVPQU databaseName: Public Health Database customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: 8C1 dateStart: 20061201 isFulltext: true titleUrlDefault: https://search.proquest.com/publichealth providerName: ProQuest – providerCode: PRVATS databaseName: Public Library of Science (PLoS) Journals Open Access customDbUrl: eissn: 1932-6203 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0053866 issn: 1932-6203 databaseCode: FPL dateStart: 20060101 isFulltext: true titleUrlDefault: http://www.plos.org/publications/ providerName: Public Library of Science
link	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV3db9MwELdYhxAvwMbHAqUYhAQ8pOTLcfK4Vq2YtpaoharjJXIcB4pCUjUtfz9nxw1k2gS8nKr60rg_-y6_i31nhF6HKcQIlpuYnNoOBCipZYYZISYLReoQRgJL7SZcXNDpNFguw-h3oHhlBd-l9nuNaX9dFqKvKD4hB-jQcX1fHtUwji72nhds1_d1etxNV7YeP6pKf-OLO-u8rK4jmlf3S_7xABrf_9-uP0D3NNXEp_XcOEK3RHGM7kz0YvoxOtJ2XeG3uvj0u4foe_1RDhcuMyxP9uAixVG-A-9SAsHe4vlW_MBDkecVlskpWK0D4Cmr4GajtczxyGFSaw21JQEzPBcQ-6_wYrXZVXihlgoeoc_j0afhB1OfxwADSYKtmYqQcUcSSmbxAEaTu1kGBItDTGX7hDKa-QBS4nuZz1gG1CijxA94wlzbEV7oPkadAqA4QVh4LOTQ5FOIZ5IkZcCLLErDlKQESFBgIHc_TDHXxcrlmRl5rFbgKAQtNYixxDbW2BrIbK5a18U6_qI_kDOg0ZWlttUXMIixttxYUJcFwuUkEKHnZU7iJDTNBNCyEPrOQwO9kPMnrvNWG4cRnxLPBQcKwBjoldKQ5TYKuZ_nK9tVVXz2cfEPSvNZS-mNVspKgIMznUMB_0mW8Wppdlua4DR4q_lEzvY9KhVg5MhMIdsF6Lt7C7i--WXTLH9U7tErRLkDHUtmJUNA7BnoSW0wDbKOE8qTBGwD0ZYptaBvtxSrb6raOdCswAnspzf3-Bm6CzTWkW_6bbeLOtvNTjxHt_nP7ara9NABnS2kXFIlA5DB0O6hw8FoGs166i1MTzkSkOeDPsiJdS4ljZScg4zIF7giOptEl78AM8Zxcw
linkProvider	Public Library of Science
linkToHtml	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1R3LbtNAcFUFBFyAlkcNhS4IBBzc2uvH2geESmjVqGmoSIl6M-v1ugQZO8QJiJ_iG5mx16ZGFXDpgVvkGTvryTwzL0KehAnECJYTm5LbDAKUxDLD1PNMEaqEecILrKqacDLko1FwchIerZAfTS8MllU2OrFS1Ekh8T_ybYiasJXBdoJXsy8mbo3C7GqzQqNmiwP1_RuEbOXLwRv4fZ8ytrd73N839VYBOI4XLMxEhUIydIuEJQM4k3TSFNwECZGB7Xtc8NRXiYx9N_WFSMHAp9zzAxkLx2bKxeFLoPIvuQ5zUIqCfltSArrD93V7nsPtbc0NW7MiV1tVcIINhWfMX7UloLUFvVlWlOc5ur_Xa54xgHs3_jfS3STXtatNd2rZWCUrKl8jVw51McEaWdV6raTP9fDtF7fIp_ojsistUoqbTaRK6FG2BO1aQICxoOOF-kz7KstKis05tMqD0JEo4ct2Z9jjkoFQa4yqJIMKOlZ5IqZ0Mp0vSzqpUiW3yfsLef07pJcDL6wTqlwRSgD5HOK5OE4E-IUW52HiJR44gYFBnIZNIqmHtePOkCyqMpAcgraaiBEyV6SZyyBme9esHlbyF_zXyIEtLo4ary4U89NIa65IcUcEypFeoELXTVnMYp6kCtzSEM4uQ4NsIv9Gdd9uqzCjHc91wIAAYQzyuMLAcSM51jOdimVZRoO3k39AGr_rID3TSGkB5JBC95DAO-EYsw7mRgcTlKbsgNdR2hqqlNEvGYE7Gyk6H_yoBeNDsUYxV8UScCzsyvbBxzfI3VpgW8oyFuImBdsgvCPKHdJ3Ifn0YzXtHdzMgAX2vT8fa5Nc3T8-HEbDwejgPrkGbj3DzIftbJDeYr5UD8hl-XUxLecPK5VFyYeLFvSffGXU1Q
linkToPdf	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1R3LbtNAcFUFVHEBWh41FLogEHBwY68fax8QKm0jopYQEYh6M-v1ugQFO8QJiF_j65hZr0ONKuDSA7fIM3bWk3lmXoQ8ijOIERwvtSV3GQQomWPHeRDYIlYZC0QQObqacHzMB4Po5CQerpEfTS8MllU2OlEr6qyU-B95F6ImbGVwvaibm7KI4UHvxeyLjRukMNParNOoWeRIff8G4Vv1vH8Av_VjxnqH7_Zf2WbDABwtiBZ2pmIhGbpIwpERnE96eQ4ug4QowQ0DLngeqkymoZ-HQuRg7HMehJFMhecy5eMgJlD_l7gfOkyXDQ4bKwB6JAxNq57H3a7hjN1ZWahdHahgc-EZU6g3BqzsQmc2LavznN7fazfPGMPetf-ZjNfJVeOC071aZjbImio2yfprU2SwSTaMvqvoUzOU-9kN8qn-iGxMy5zixhOpMjqcLkHrlhB4LOhooT7TfTWdVhSbdqjOj9CBqODLDmfY-zIFYTcYulSDCjpSRSYmdDyZLys61imUm-T9hbz-LdIpgC-2CFW-iCWAQg5xXppmAvxFh_M4C7IAnMPIIl7DMok0Q9xxl8g00ZlJDsFcTcQEGS0xjGYRe3XXrB5i8hf8l8iNK1wcQa4vlPPTxGi0RHFPRMqTQaRi389ZylKe5Qrc1RjOLmOL7CAvJ3U_70qRJnuB74FhAcJY5KHGwDEkBXLiqVhWVdJ_M_4HpNHbFtITg5SXQA4pTG8JvBOON2thbrcwQZnKFngLJa-hSpX8khe4s5Go88EPVmB8KNYuFqpcAo6D3doh-P4WuV0L74qyjMW4YcG1CG-JdYv0bUgx-ainwIP7GbHIvfPnY-2QdZDv5Lg_OLpLroC3zzAh4nrbpLOYL9U9cll-XUyq-X2tvSj5cNFy_hNt3d3h
openUrl	ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Generation+of+Induced+Pluripotent+Stem+Cells+from+Human+Nasal+Epithelial+Cells+Using+a+Sendai+Virus+Vector&rft.jtitle=PloS+one&rft.au=Ono%2C+Mizuho&rft.au=Hamada%2C+Yuko&rft.au=Horiuchi%2C+Yasue&rft.au=Matsuo-Takasaki%2C+Mami&rft.date=2012-08-13&rft.pub=Public+Library+of+Science&rft.issn=1932-6203&rft.eissn=1932-6203&rft.volume=7&rft.issue=8&rft.spage=e42855&rft_id=info:doi/10.1371%2Fjournal.pone.0042855&rft.externalDBID=IOV&rft.externalDocID=A543307165
thumbnail_l	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1932-6203&client=summon
thumbnail_m	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1932-6203&client=summon
thumbnail_s	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1932-6203&client=summon